Accessing Single Nucleotide Polymorphisms in Genomic DNA by Direct Multiplex Polymerase Chain Reaction Amplification on Oligonucleotide Microarrays

2002 ◽  
Vol 303 (1) ◽  
pp. 25-33 ◽  
Author(s):  
Martin Huber ◽  
Axel Mündlein ◽  
Eva Dornstauder ◽  
Christian Schneeberger ◽  
Clemens B. Tempfer ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Genomic Dna ◽  
Multiplex Polymerase Chain Reaction ◽  
Polymerase Chain Reaction Amplification ◽  
Oligonucleotide Microarrays ◽  
Nucleotide Polymorphisms ◽  
Chain Reaction ◽  
Single Nucleotide ◽  
Reaction Amplification ◽  
Polymerase Chain

scholarly journals P-SELECTIN GLYCOPROTEIN LIGAND-1 GENE POLYMORPHISMS IN SUDANESE PREGNANT WOMEN WITH THROMBOSIS

2018 ◽  
Vol 11 (12) ◽  
pp. 226
Author(s):  
Abdelrahman A Elhassan ◽  
Fathelrahman Mahdi Hassan ◽  
Hanan B Eltahir
Keyword(s):  
Polymerase Chain Reaction ◽  
Pregnant Women ◽  
Gene Polymorphisms ◽  
Genomic Dna ◽  
Polymerase Chain Reaction Amplification ◽  
Healthy Controls ◽  
Chain Reaction ◽  
Increased Risk ◽  
Reaction Amplification ◽  
Polymerase Chain

Objectives: In this study we aimed to investigate whether P-selectin gene polymorphism is associated with thrombosis in Sudanese pregnant women in Khartoum state.Methods: After informed consent, 96 Sudanese pregnant women with thrombosis and 53 healthy pregnant women were recruited in the study. Genomic DNA was isolated from whole blood. Genotyping of PSGL-1 gene was performed by polymerase chain reaction amplification for exon 14 and electrophoresis.Results: The frequency of the B allele was found to be significantly higher in pregnant women with thrombosis (26%) compared to the controls (17.4%).Conclusion: The AB genotype was found to be higher in women with thrombosis 40.6% than in healthy controls 20% (p=0.02). Our results suggest that the PSGL-1 AB genotype is associated with an increased risk of thrombosis in Sudanese pregnant women.

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