Partially Fertile Line with Apospory Obtained from Tissue Culture of Male Sterile Plant of Sorghum (Sorghum bicolor L. Moench)

SUMMARYMale-sterile, female-fertile plants were used to produce hybrid soybean seed. Manual cross-pollination using male-sterile plants to produce large quantities of hybrid seed is difficult and time-consuming because of the low success rate in cross-pollination. Insect pollinators may be suitable vectors to transfer pollen, but the most suitable vector for pollen transfer from the male parent to the female parent has not been identified for soybean. The objective of the present study was to evaluate seed-set on four male-sterile, female-fertile soybean lines by using alfalfa leafcutting bees (Megachile rotundata (F.)) and honey bees (Apis mellifera (L.)) as pollinators. Seed-set was evaluated in summers 2003 and 2005 near Ames, Iowa, USA and in summers 2003, 2004, and 2005 near Wooster, Ohio, USA. Neither the effect of pollinator species nor the interaction effect of pollinator species×location was significant for any year. Honey bees performed similarly to alfalfa leafcutting bees at both locations. The results indicated significant differences for seed-set among male-sterile lines, suggesting preferential pollination. Male-sterile lines, ms1 (Urbana) and ms2 (Ames 2), had higher cross-pollinated seed-set compared to ms6 (Ames 1), and ms6 (Corsoy 79). At the Ames location, ms1ms1 (Urbana) plants had the highest seed-set (50·16 seeds per male-sterile plant in 2005). At the Wooster location, ms1ms1 (Urbana) plants also had the highest seed-set (92·04 seeds per male-sterile plant) in 2005. Costs and local conditions need to be addressed to support the choice of either pollinator species as a pollination vector to produce hybrid soybean seed.

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DISTANCE FROM POLLEN SOURCE AND YIELDS OF MALE STERILE SUNFLOWER AND SORGHUM

Canadian Journal of Plant Science ◽

10.4141/cjps84-117 ◽

1984 ◽

Vol 64 (4) ◽

pp. 857-861 ◽

Cited By ~ 2

Author(s):

ROBERT G. ROBINSON

Keyword(s):

Sorghum Bicolor ◽

Helianthus Annuus ◽

Seed Yield ◽

Efficient Production ◽

Production Plant ◽

Hybrid Seed Production ◽

Male Sterile ◽

Helianthus Annuus L ◽

Sterile Seed ◽

Sorghum Bicolor L

Efficient production of hybrid sunflower (Helianthus annuus L.) and sorghum (Sorghum bicolor (L.) Moench) seed requires a minimum but adequate proportion of the land for pollinator rows and that the male sterile and pollinator rows accommodate production machinery. The objective of this research was to determine the effect of distance from pollen source on male sterile seed yield and if presently used ratios of male sterile to pollinator rows could be increased without reduction in seed yield of the male sterile parent from the land area occupied by both parents. Seed was harvested from male sterile rows located from 1 (76 cm) to 40 (30 m) rows from the pollen source. Correlations and regression analyses showed that yield per row declined linearly with distance from the pollinator rows. However, yield of the male sterile parent per hectare of area occupied by both parents was increased by including up to at least six male sterile and two pollinator rows per strip. Male sterile to pollinator row ratios of 12:1 for sunflower and 7:1 for grain sorghum did not differ significantly from the commonly used 3:1 ratio in yield of male sterile parent per hectare of area occupied by both parents.Key words: Helianthus annuus L., Sorghum bicolor (L.) Moench, hybrid seed production, plant breeding

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Identification of stable restorers for diverse cytoplasmic source in Sorghum [Sorghum bicolor (L.) Moench.]

Indian Journal of Genetics and Plant Breeding (The) ◽

10.31742/ijgpb.80.2.14 ◽

2020 ◽

Vol 80 (02) ◽

Author(s):

N. Sandeep ◽

Basavaraj Biradar

Keyword(s):

Cytoplasmic Male Sterility ◽

Sorghum Bicolor ◽

Seed Set ◽

Fertility Restoration ◽

Male Sterile ◽

Commercial Exploitation ◽

M 31 ◽

Fertility Restorers ◽

Sorghum Bicolor L ◽

Sorghum Germplasm

In sorghum [Sorghum bicolor (L.) Moench.], milo is the lone source of cytoplasmic male sterility (CMS) extensively used in production of commercial hybrids. The hazards of such a narrow cytoplasmic base are apparent and consequently new diverse sources like A2, A3, A4, maldandi, VZM and G1 were identified. However, the commercial exploitation of these sources has not been possible because of the difficulty in fertility restoration on these sources. The present study was, therefore, undertaken to test different sorghum germplasm lines for their fertility restoration/maintenance on maldandi and milo cytoplasm. Twenty-five diverse accession were selected from minicore collection and crossed with two male sterile lines viz., 104A (milo) and M 31-2A (maldandi). The resulting F1s were classified as fertility restorers and maintainers based on seed set on bagged ear heads. The restoration studies indicated that 2 lines on maldandi and 6 on milo cytoplasm showed strong fertility restoration (> 90 % seed set).

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Evaluation of Seven Sorghums, Selfed and Crossed to Three Cytoplasmic Male-Sterile Lines

The Journal of Agriculture of the University of Puerto Rico ◽

10.46429/jaupr.v62i2.10376 ◽

1969 ◽

Vol 62 (2) ◽

pp. 156-164

Author(s):

A. Sotomayor-Ríos ◽

D. E. Weibel

Keyword(s):

Grain Yield ◽

Sorghum Bicolor ◽

Grain Sorghum ◽

Test Weight ◽

F1 Hybrids ◽

Male Sterile ◽

Cytoplasmic Male Sterile ◽

Complementary Effect ◽

Sorghum Bicolor L ◽

High Test

Seven grain sorghum lines (Sorghum bicolor (L.) Moench) selected from the Sorghum Conversion Program (SCP) and their F1 hybrids from crosses with CK-60, Martin, and Redlan were evaluated. Days to midflower, height, rust (Puccinia purpurea Ckd.) reaction, number of heads/plot, test weight, threshing percentage, and grain yield were rated or measured. The seven lines when selfed were, in general, the latest to reach midf lower. Four height groups were observed; the taller hybrids were the result of the complementary effect of different height factors in the parents. Rust ratings ranged from 2.9 to 4.9; F1 hybrids Martin x 4, Martin x 7, and Redlan x 7 were the most susceptible to rust. Generally, sorghums with a high grain yield also had a high test weight and thresh ing percentage. The grain yield ranged from 1,866 to 4,261 kg/ha. Mean yields were, on the average, 26% higher for F1 hybrids than for their respective male parents. A 67% heterosis for grain yield was observed in two crosses invo lving Martin and Redlan female parents.

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