Pilot Testing of Pichia guilliermondii: A Biocontrol Agent of Postharvest Diseases of Citrus Fruit

1993 ◽  
Vol 3 (1) ◽  
pp. 47-52 ◽  
Author(s):  
S. Droby ◽  
R. Hofstein ◽  
C.L. Wilson ◽  
M. Wisniewski ◽  
B. Fridlender ◽  
...  
Keyword(s):  
Biocontrol Agent ◽  
Citrus Fruit ◽  
Postharvest Diseases ◽  
Pichia Guilliermondii ◽  
Pilot Testing

Control of postharvest diseases on citrus fruit by preharvest application of the biocontrol agent Pantoea agglomerans CPA-2

2008 ◽  
Vol 49 (1) ◽  
pp. 86-95 ◽  
Author(s):  
Teresa Paula Cañamás ◽  
Immaculada Viñas ◽  
Josep Usall ◽  
Carla Casals ◽  
Cristina Solsona ◽  
...  
Keyword(s):  
Biocontrol Agent ◽  
Citrus Fruit ◽  
Pantoea Agglomerans ◽  
Postharvest Diseases

Control of postharvest diseases on citrus fruit by preharvest applications of biocontrol agent Pantoea agglomerans CPA-2

2008 ◽  
Vol 49 (1) ◽  
pp. 96-106 ◽  
Author(s):  
Teresa P. Cañamás ◽  
Immaculada Viñas ◽  
Josep Usall ◽  
Rosario Torres ◽  
Marina Anguera ◽  
...  
Keyword(s):  
Biocontrol Agent ◽  
Citrus Fruit ◽  
Pantoea Agglomerans ◽  
Postharvest Diseases

scholarly journals Identification of Lasiodiplodia pseudotheobromae Causing Fruit Rot of Citrus in China

Plants ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 202
Author(s):  
Jianghua Chen ◽  
Zihang Zhu ◽  
Yanping Fu ◽  
Jiasen Cheng ◽  
Jiatao Xie ◽  
...  
Keyword(s):  
Phylogenetic Analyses ◽  
Elongation Factor ◽  
Morphological Characteristics ◽  
Citrus Fruit ◽  
Economic Loss ◽  
Fruit Rot ◽  
Post Inoculation ◽  
Citrus Reticulata ◽  
Postharvest Diseases ◽  
Citrus Reticulata Blanco

Considering the huge economic loss caused by postharvest diseases, the identification and prevention of citrus postharvest diseases is vital to the citrus industry. In 2018, 16 decayed citrus fruit from four citrus varieties—Satsuma mandarin (Citrus unshiu), Ponkan (Citrus reticulata Blanco cv. Ponkan), Nanfeng mandarin (Citrus reticulata cv. nanfengmiju), and Sugar orange (Citrus reticulata Blanco)—showing soft rot and sogginess on their surfaces and covered with white mycelia were collected from storage rooms in seven provinces. The pathogens were isolated and the pathogenicity of the isolates was tested. The fungal strains were identified as Lasiodiplodia pseudotheobromae based on their morphological characteristics and phylogenetic analyses using the internal transcribed spacer regions (ITS), translation elongation factor 1-α gene (TEF), and beta-tubulin (TUB) gene sequences. The strains could infect wounded citrus fruit and cause decay within two days post inoculation, but could not infect unwounded fruit. To our knowledge, this is the first report of citrus fruit decay caused by L. pseudotheobromae in China.

Pilot testing ofKloeckera apiculata for the biological control of postharvest diseases of citrus

2006 ◽  
Vol 56 (1) ◽  
pp. 13-17 ◽  
Author(s):  
Chao-An Long ◽  
Bo-Xun Deng ◽  
Xiu-Xin Deng
Keyword(s):  
Biological Control ◽  
Postharvest Diseases ◽  
Pilot Testing

Effects of Pichia guilliermondii and Hot Air Treatment on the Postharvest Preservation of Red Fuji Apple Quality Attributes

2018 ◽  
Vol 81 (2) ◽  
pp. 186-194 ◽  
Author(s):  
Yan Zhao ◽  
Jingjing Yin
Keyword(s):  
Apple Fruit ◽  
Penicillium Expansum ◽  
Total Phenolic ◽  
Postharvest Diseases ◽  
Pichia Guilliermondii ◽  
Air Treatment ◽  
Hot Air ◽  
Antagonistic Yeast ◽  
Hot Air Treatment ◽  
Apple Quality

ABSTRACT The effects of individual and combined Pichia guilliermondii (at 1 × 108 CFU mL−1) and hot air (at 38°C for 96 h) treatments on the three major postharvest diseases Botrytis cinerea, Penicillium expansum, and Colletotrichum gloeosporioides, as well as the quality and antioxidant content of Red Fuji (Malus pumila var. domestica) apple fruit, were investigated in this work. Results suggested that the combined hot air and antagonistic yeast (P. guilliermondii) treatment effectively and completely inhibited the infection of apple fruit wounds by the three major postharvest diseases. Furthermore, apple fruit treated with antagonistic yeast or heat alone maintained better quality, which included mass loss, firmness, solid/acid ratio, and ascorbic acid content, than the control. The combination of the two treatments yielded the optimum apple quality. Moreover, the combined hot air and P. guilliermondii treatment also maintained or enhanced the antioxidative enzyme activities and total phenolic content of apple fruit. All results demonstrated that the combined antagonistic yeast and hot air treatment maintained the postharvest freshness of apple fruit.

scholarly journals Development of molecular tools for the yeast Papiliotrema terrestris LS28 and identification of Yap1 as a transcription factor involved in biocontrol activity

2021 ◽  
Author(s):  
Raffaello Castoria ◽  
Cecilia Miccoli ◽  
Giuseppe Barone ◽  
Davide Palmieri ◽  
Filippo De Curtis ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Biocontrol Agent ◽  
Integrated Control ◽  
Biocontrol Agents ◽  
Alternative Methods ◽  
Penicillium Expansum ◽  
Postharvest Disease ◽  
Molecular Tools ◽  
Postharvest Diseases ◽  
Biocontrol Activity

Fungal attacks on stored vegetable and fruits are responsible for losses of products. There is an active research field to develop alternative strategies for postharvest disease management, and the use of biocontrol agents represents a promising approach. Understanding the molecular bases of the biocontrol activity of these agents is crucial to potentiate their effectiveness. The yeast Papiliotrema terrestris is a biocontrol agent against postharvest pathogens. Phenotypic studies suggest that it exerts its antagonistic activity through competition for nutrients and space, which relies on its resistance to oxidative and other cellular stresses. In this study, we developed tools for genetic manipulation in P. terrestris to perform targeted gene replacement and functional complementation of the transcription factors Yap1 and Rim101. In vitro phenotypic analyses revealed a conserved role of Yap1 and Rim101 in broad resistance to oxidative stress and alkaline pH sensing, respectively. In vivo analyses revealed that P. terrestris yap1Δ and rim101Δ mutants display decreased ability to colonize wounded fruit as compared to the parental WT strain; the yap1Δ mutant also displays reduced biocontrol activity against the postharvest pathogens Penicillium expansum and Monilia fructigena, indicating an important role for resistance to oxidative stress in timely wound colonization and biocontrol activity of P. terrestris. In conclusion, the availability of molecular tools developed in the present study provides a foundation to elucidate the genetic mechanisms underlying biocontrol activity of P. terrestris, with the goal of enhancing this activity for the practical use of P. terrestris LS28 in pest management programs based on biological and integrated control. IMPORTANCE The use of fungicides represents the most effective and widely used strategy for controlling postharvest diseases. However, their extensive use has raised several concerns, such as the emergence of plant pathogens’ resistance as well as the health risks associated with the persistence of chemical residues in fruits, vegetables, and in the environment. These factors have brought attention to alternative methods for controlling postharvest diseases, such as the utilization of biocontrol agents. In the present study we developed genomics and genetics resources to investigate at molecular level the mechanisms involved in the biocontrol activity of Papiliotrema terrestris, a basidiomycete yeast that is an effective biocontrol agent against widespread fungal pathogens, including Penicillium expansum, the etiological agent of blue mold disease of pome fruits. A deeper understanding of how postharvest biocontrol agents operate is the basic requirement to promote the utilization of biological (and integrated) control for the reduction of chemical fungicides.

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