Cultured Murine Parenchymal Liver Cells Induce Differentiation of Bone Marrow Cells to Macrophage-like Cells Which Present Antigen to Th1 Clones but Inhibit Their Proliferation by Nitric Oxide and Prostaglandins

1999 ◽  
Vol 196 (1) ◽  
pp. 14-22 ◽  
Author(s):  
Ayako Mabuchi ◽  
Masumi Kitajima-Shimizu ◽  
Kyoko Kikuchi ◽  
Yohko Nakagawa ◽  
Hidemi Takahashi ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Liver Cells ◽  
Parenchymal Liver ◽  
Marrow Cells

scholarly journals In vivo genotoxicity of treated water containing the cylindrospermopsin-producer Cylindrospermopsis raciborskii

2014 ◽  
Vol 12 (3) ◽  
pp. 474-483 ◽  
Author(s):  
A. L. Fonseca ◽  
J. Da Silva ◽  
E. A. Nunes ◽  
S. M. F. O. Azevedo ◽  
R. M. Soares
Keyword(s):  
Bone Marrow ◽  
Comet Assay ◽  
Bone Marrow Cells ◽  
Micronucleus Test ◽  
Liver Cells ◽  
Cylindrospermopsis Raciborskii ◽  
Producer Strain ◽  
Treated Water ◽  
Marrow Cells

Cylindrospermopsin (CYN) is an alkaloid commonly produced by some cyanobacteria that has been implicated in outbreaks of human illness. The aim of this study was to investigate the genotoxicity of Cylindrospermopsis raciborskii cellular content (including CYN) and its byproducts resulting from chlorination during water treatment. DNA damage in blood and liver cells was analysed by the comet assay and micronucleus test (MN). Mice were injected intraperitoneally with the following treatments: (a) physiological saline, (b) treated water, (c) treated water plus C. raciborskii extract (CYN producer strain, CYPO-011 K), (d) C. raciborskii extract (CYN producer strain, CYPO-011 K), (e) C. raciborskii extract (CYN non producer strain), and (f) treated water plus C. raciborskii extract (CYN non producer strain) extract. After 48 h, samples were taken to perform tests (blood and liver cells to the comet assay and bone marrow to MN test). The CYPO-011 K had a genotoxic and mutagenic effects on liver and bone marrow cells. The group that received chlorine-treated water plus CYPO-011 K also exhibited genotoxic effects in the liver, as well as in the blood, and a mutagenic effect in blood marrow cells. The results emphasise the need of improving CYN monitoring in waters bodies in order to reduce the risk of human exposure.

scholarly journals Mouse fetal liver cells lack functional amphotropic retroviral receptors

Blood ◽  
1994 ◽  
Vol 84 (2) ◽  
pp. 433-439 ◽  
Author(s):  
C Richardson ◽  
M Ward ◽  
S Podda ◽  
A Bank
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Fetal Liver ◽  
Hematopoietic Cells ◽  
Retroviral Vectors ◽  
Liver Cells ◽  
Mouse Bone Marrow ◽  
Adult Mice ◽  
Fetal Liver Cells ◽  
Marrow Cells

Abstract We have been transducing mouse hematopoietic cells with the human MDR1 (MDR) gene in retroviral vectors to determine the optimal conditions for retroviral gene transfer as a model system for potential human gene therapy. In these studies, we have demonstrated transduction and expression of the human MDR gene using ecotropic and amphotropic MDR- retroviral producer lines. To obtain more mouse hematopoietic cells for detailed study, mouse fetal liver cells (FLC) have been used for MDR transduction and expression, and to reconstitute the ablated marrows of live adult mice. FLC contain hematopoietic cells that have a reconstituting capacity comparable to that of adult mouse bone marrow cells. However, to our surprise, FLC can only be transduced with ecotropic retrovirus and not with amphotropic virus. This restriction of transduction of FLC cannot be overcome by higher titer virus. The resistance to amphotropic transduction by FLC may be part of a changing developmental program that results in a different antigen repertoire on FLC as compared with adult bone marrow cells.

IL-12- and IL-18-Mediated, Nitric Oxide-Induced Apoptosis in TNF-α-Mediated Osteoclastogenesis of Bone Marrow Cells

2011 ◽  
Vol 89 (1) ◽  
pp. 65-73 ◽  
Author(s):  
Hideki Kitaura ◽  
Yuji Fujimura ◽  
Masako Yoshimatsu ◽  
Haruka Kohara ◽  
Yukiko Morita ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Tnf Α ◽  
Induced Apoptosis ◽  
Marrow Cells

scholarly journals Mouse fetal liver cells lack functional amphotropic retroviral receptors

Blood ◽  
1994 ◽  
Vol 84 (2) ◽  
pp. 433-439
Author(s):  
C Richardson ◽  
M Ward ◽  
S Podda ◽  
A Bank
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Fetal Liver ◽  
Hematopoietic Cells ◽  
Retroviral Vectors ◽  
Liver Cells ◽  
Mouse Bone Marrow ◽  
Adult Mice ◽  
Fetal Liver Cells ◽  
Marrow Cells

We have been transducing mouse hematopoietic cells with the human MDR1 (MDR) gene in retroviral vectors to determine the optimal conditions for retroviral gene transfer as a model system for potential human gene therapy. In these studies, we have demonstrated transduction and expression of the human MDR gene using ecotropic and amphotropic MDR- retroviral producer lines. To obtain more mouse hematopoietic cells for detailed study, mouse fetal liver cells (FLC) have been used for MDR transduction and expression, and to reconstitute the ablated marrows of live adult mice. FLC contain hematopoietic cells that have a reconstituting capacity comparable to that of adult mouse bone marrow cells. However, to our surprise, FLC can only be transduced with ecotropic retrovirus and not with amphotropic virus. This restriction of transduction of FLC cannot be overcome by higher titer virus. The resistance to amphotropic transduction by FLC may be part of a changing developmental program that results in a different antigen repertoire on FLC as compared with adult bone marrow cells.

scholarly journals Identification of hemopoietic cells responsive to colony-stimulating factor by autoradiography

Blood ◽  
1983 ◽  
Vol 62 (6) ◽  
pp. 1197-1202
Author(s):  
RK Shadduck ◽  
G Pigoli ◽  
C Caramatti ◽  
G Degliantoni ◽  
V Rizzoli ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Mononuclear Cells ◽  
Bone Marrow Cells ◽  
Fetal Liver ◽  
Liver Cells ◽  
Colony Stimulating Factor ◽  
Murine Bone Marrow ◽  
Fetal Liver Cells ◽  
Stimulating Factor ◽  
Marrow Cells

Binding of radiolabeled L-cell colony-stimulating factor (CSF) was studied using murine bone marrow and fetal liver cells. With 10(7) cells, saturation of binding was seen with approximately 500,000 cpm of 125I-CSF. Minimal binding was detected after one hour incubation with tracer at 37 degrees C; however, marked cellular uptake of radioactivity was noted after 24-hr exposure to CSF. As judged by autoradiographs, small numbers of myeloblasts, promyelocytes, and large mononuclear cells were labeled with 1-hr exposure to tracer. By 6 hr of incubation, 50%-70% of myeloblasts and promyelocytes and small numbers of late granulocytic cells were labeled. Virtually all myeloblasts and promyelocytes and approximately 50% of myelocytes, metamyelocytes, polymorphonuclear granulocytes, and monocytes were labeled after 24-hr exposure to the radioiodinated CSF. Label was not detected on erythroblasts, eosinophils, or megakaryocytes. Suspensions of fetal liver cells had lower uptake of radioactivity than bone marrow cells. This appeared to result from a lesser concentration of granulocytic cells in fetal liver, as labeling of individual cells was similar with both tissues. In additional experiments, CSF binding to marrow cells was assessed after 30-min exposure to tracer at 0 degrees C. Uptake of 125I-CSF exceeded that observed after 24-hr incubation at 37 degrees C. With this technique, cellular label was also confined to granulocytic and monocytic cells. These findings suggest that purified CSF reacts with and may stimulate immature and mature cells of the granulocytic and monocytic lineages.

Sign in / Sign up

Export Citation Format

Share Document