A Comprehensive Evaluation of the Effects and Mechanisms of Antifreeze Proteins during Low-Temperature Preservation

Cryobiology ◽  
2000 ◽  
Vol 41 (1) ◽  
pp. 1-9 ◽  
Author(s):  
Jun-Hui Wang
2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Yi Lu ◽  
Jianming Zheng ◽  
Chen Zhao ◽  
Gang Ren

When using the risk priority number method to perform a criticality analysis of the failure modes of mechanical products, different subjective factors exist on the part of experts regarding the severity level (S), occurrence probability level (O), and failure modes. The estimation of the detection difficulty level (D) is different, which causes the problem of inaccurate evaluation of the criticality of failure modes. This study proposes a harm analysis method that combines group decision-making and fuzzy comprehensive evaluation. The similarity and difference between an individual expert and the group are used in group decision-making to assign the weight of the expert, thus reducing the influence of subjective factors on the evaluation results. On the basis of the fuzzy comprehensive evaluation method, the weight of three risk elements (S, O, and D) is determined, and a hazard ranking of the interval obtained by group decision-making is performed. The CA (criticality analysis) improvement method is used to analyze the hazard posed by low-temperature shut-off valves. Results indicate that this method can effectively assess the weak links of the low-temperature shut-off valves and improve the accuracy of the hazard evaluation in comparison with the risk priority coefficient method.


Agronomy ◽  
2019 ◽  
Vol 9 (2) ◽  
pp. 61 ◽  
Author(s):  
Yun Dai ◽  
Lingyun Yuan ◽  
Shujiang Zhang ◽  
Jie Wang ◽  
Shilei Xie ◽  
...  

Wucai is a biannual leafy vegetable that is more sensitive to cold than most Brassicaceae vegetables. Here, phenotypic differences in wucai were investigated to identify those genotypes with high cold tolerance at low temperature, which could allow their cultivation in cold climates. In all, on the basis of PIabs, 20 wucai genotypes (10 lower PIabs and 10 higher PIabs) were selected from a collection of 124 genotypes. Both W16-13 and SW-3 (higher PIabs) and the LS-6 and W15-16 (lower PIabs) were screened from the 20 wucai genotypes according to several key plant physiological traits: net photosynthetic rate, electrolyte leakage, chilling injury index, specific leaf area, malondialdehyde contents, and PIabs. To further verify the reliability of PIabs, four genotypes were grown under low temperature gradient conditions and their morphological indexes, chlorophyll content, total antioxidant capacity, fluorescence transients (OJIP transients), leaf cell ultrastructure, and gene expression were measured. These indicators strongly demonstrated that the W16-13 and SW-3, which had higher PIabs, possessed higher resistance to cold stress, while both LS-6 and W15-16 were sensitive to cold. As this study shows, the easily measured performance index, PIabs, could be applied to wucai genotypes to screen for one or more varieties characterized by higher cold tolerance.


2020 ◽  
Vol 8 (18) ◽  
pp. 9373-9381 ◽  
Author(s):  
Jiajun Xu ◽  
Rining Jing ◽  
Xiuyan Ren ◽  
Guanghui Gao

Breaking through the conventional way of conferring anti-icing ability on hydrogels with addition of organic solvents or inorganic salts, a novel anti-icing hydrogel driven by antifreeze proteins was successfully fabricated and applied as sensors.


BIOS ◽  
2019 ◽  
Vol 90 (3) ◽  
pp. 158
Author(s):  
Steven N. Bagwell ◽  
Josette V. Ricker

2017 ◽  
Vol 18 (sup4) ◽  
pp. 467-490 ◽  
Author(s):  
Yuefeng Zhu ◽  
Eshan V. Dave ◽  
Reyhaneh Rahbar-Rastegar ◽  
Jo Sias Daniel ◽  
Adam Zofka

2016 ◽  
Vol 113 (14) ◽  
pp. 3740-3745 ◽  
Author(s):  
Luuk L. C. Olijve ◽  
Konrad Meister ◽  
Arthur L. DeVries ◽  
John G. Duman ◽  
Shuaiqi Guo ◽  
...  

Antifreeze proteins (AFPs) are a unique class of proteins that bind to growing ice crystal surfaces and arrest further ice growth. AFPs have gained a large interest for their use in antifreeze formulations for water-based materials, such as foods, waterborne paints, and organ transplants. Instead of commonly used colligative antifreezes such as salts and alcohols, the advantage of using AFPs as an additive is that they do not alter the physicochemical properties of the water-based material. Here, we report the first comprehensive evaluation of thermal hysteresis (TH) and ice recrystallization inhibition (IRI) activity of all major classes of AFPs using cryoscopy, sonocrystallization, and recrystallization assays. The results show that TH activities determined by cryoscopy and sonocrystallization differ markedly, and that TH and IRI activities are not correlated. The absence of a distinct correlation in antifreeze activity points to a mechanistic difference in ice growth inhibition by the different classes of AFPs: blocking fast ice growth requires rapid nonbasal plane adsorption, whereas basal plane adsorption is only relevant at long annealing times and at small undercooling. These findings clearly demonstrate that biomimetic analogs of antifreeze (glyco)proteins should be tailored to the specific requirements of the targeted application.


2021 ◽  
Vol 176 ◽  
pp. 94-103
Author(s):  
Lucas F.L. Correia ◽  
Bruna R.C. Alves ◽  
Ribrio I.T.P. Batista ◽  
Pascal Mermillod ◽  
Joanna M.G. Souza-Fabjan

2020 ◽  
Vol 47 (3) ◽  
pp. 203-208
Author(s):  
Phyo Phyo Win Pe ◽  
Swum Yi Kyua ◽  
Aung Htay Naing ◽  
Kyeung Il Park ◽  
Mi-Young Chung ◽  
...  

Author(s):  
P.P.K. Smith

Grains of pigeonite, a calcium-poor silicate mineral of the pyroxene group, from the Whin Sill dolerite have been ion-thinned and examined by TEM. The pigeonite is strongly zoned chemically from the composition Wo8En64FS28 in the core to Wo13En34FS53 at the rim. Two phase transformations have occurred during the cooling of this pigeonite:- exsolution of augite, a more calcic pyroxene, and inversion of the pigeonite from the high- temperature C face-centred form to the low-temperature primitive form, with the formation of antiphase boundaries (APB's). Different sequences of these exsolution and inversion reactions, together with different nucleation mechanisms of the augite, have created three distinct microstructures depending on the position in the grain.In the core of the grains small platelets of augite about 0.02μm thick have farmed parallel to the (001) plane (Fig. 1). These are thought to have exsolved by homogeneous nucleation. Subsequently the inversion of the pigeonite has led to the creation of APB's.


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