Tyrosine Kinase Cell Signaling Pathways of Rat Mesangial Cells in 3-Dimensional Cultures: Response to Fetal Bovine Serum and Platelet-Derived Growth Factor-BB

1998 ◽  
Vol 240 (1) ◽  
pp. 134-143 ◽  
Author(s):  
Roy Zent ◽  
Menachem Ailenberg ◽  
Melvin Silverman
Keyword(s):  
Growth Factor ◽  
Tyrosine Kinase ◽  
Signaling Pathways ◽  
Bovine Serum ◽  
Mesangial Cells ◽  
Fetal Bovine Serum ◽  
Platelet Derived Growth Factor ◽  
3 Dimensional ◽  
Fetal Bovine ◽  
Cell Signaling Pathways

Autocrine production of matrix metalloproteinase-2 is required for human airway smooth muscle proliferation

1999 ◽  
Vol 277 (6) ◽  
pp. L1109-L1117 ◽  
Author(s):  
Simon Johnson ◽  
Alan Knox
Keyword(s):  
Smooth Muscle ◽  
Growth Factor ◽  
Airway Smooth Muscle ◽  
Fetal Bovine Serum ◽  
Platelet Derived Growth Factor ◽  
Airway Smooth Muscle Cells ◽  
Human Airway Smooth Muscle ◽  
Human Airway ◽  
Smooth Muscle Proliferation ◽  
Fetal Bovine

Airway smooth muscle proliferation is important in asthma and is dependent on pro- and antimitogenic factors and cell-matrix interactions. Here we show an antiproliferative effect of protease inhibitors on human airway smooth muscle due to inhibition of autocrine-derived matrix metalloproteinase (MMP)-2. Proliferation in response to fetal bovine serum, thrombin, and platelet-derived growth factor was inhibited by the broad-spectrum protease inhibitor Complete and the MMP inhibitors EDTA and Ro-31-9790 but not by cysteine or serine protease inhibitors. Conditioned medium from airway smooth muscle cells contained 72-kDa gelatinase that was secreted by growth-arrested cells and increased by fetal bovine serum but not by thrombin or platelet-derived growth factor. Immunostaining of cultured human airway smooth muscle cells and normal lung biopsies confirmed this gelatinase to be MMP-2. Our results suggest a novel role for MMP-2 as an important autocrine factor required for airway smooth muscle proliferation. Inhibition of MMPs could provide a target for the prevention of smooth muscle hyperplasia and airway remodeling in asthma.

scholarly journals Bypass of the ts block of tsJT60, a G0-specific ts mutant from rat fibroblasts, by fetal bovine serum and epidermal growth factor.

1987 ◽  
Vol 12 (5) ◽  
pp. 421-432 ◽  
Author(s):  
Jun Ninomiva-Tsuii ◽  
Yuko Nakahara ◽  
Chiho Ito ◽  
Taishin Akiyama ◽  
Sadahiko Ishibashi
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Epidermal Growth ◽  
Rat Fibroblasts ◽  
Ts Mutant ◽  
Fetal Bovine

Identification of a Growth Factor Mimicking the Synergistic Effect of Fetal Bovine Serum on BMP-9 Cell Response

2014 ◽  
Vol 20 (17-18) ◽  
pp. 2524-2535 ◽  
Author(s):  
Marc-Antoine Lauzon ◽  
Alex Daviau ◽  
Olivier Drevelle ◽  
Bernard Marcos ◽  
Nathalie Faucheux
Keyword(s):  
Growth Factor ◽  
Synergistic Effect ◽  
Cell Response ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Fetal Bovine
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