Juvenile Hormone-Dependent LHPI and RNA Synthesis in Melanoplus sanguinipes Long Hyaline Tubule: Events Associated with the "Insensitive Period"

1995 ◽  
Vol 98 (1) ◽  
pp. 87-93 ◽  
Author(s):  
Sheikh M. Ismail ◽  
Preeti M. Ismail ◽  
Cedric Gillott
Keyword(s):  
Juvenile Hormone ◽  
Rna Synthesis ◽  
Melanoplus Sanguinipes

Juvenile hormone biosynthesis during sexual maturation and after mating in the adult male migratory grasshopper, Melanoplus sanguinipes

1985 ◽  
Vol 63 (12) ◽  
pp. 2789-2792 ◽  
Author(s):  
G. A. Couche ◽  
C. Gillott ◽  
S. S. Tobe ◽  
R. Feyereisen
Keyword(s):  
Juvenile Hormone ◽  
Adult Male ◽  
Sexual Maturation ◽  
Adult Emergence ◽  
Corpora Allata ◽  
Melanoplus Sanguinipes ◽  
Juvenile Hormone Iii ◽  
Juvenile Hormone Biosynthesis ◽  
Single Mating ◽  
Hormone Biosynthesis

Juvenile hormone III is the exclusive juvenile hormone produced by the corpora allata in adult male Melanoplus sanguinipes. For the first 5 days after adult emergence, the rate of juvenile hormone biosynthesis is low, it then increases rapidly to peak on day 7. Between days 7 and 12 the rate declines to that of newly emerged males and then increases again in 14-day insects. In males allowed a single mating on day 7, the rate of juvenile hormone biosynthesis remains elevated at least to day 12.

Juvenile hormone and RNA synthesis in pupal tissues of saturniid moths

1966 ◽  
Vol 12 (1) ◽  
pp. 37-41 ◽  
Author(s):  
H. Oberlander ◽  
H.A. Schneiderman
Keyword(s):  
Juvenile Hormone ◽  
Rna Synthesis

XVIII. Alterations of the Nature of RNA Synthesized in Isolated Fat Body Cell Nuclei as a Result of Ecdysone and Juvenile Hormone Action

1970 ◽  
Vol 25 (3) ◽  
pp. 279-284 ◽  
Author(s):  
L. F. Congote ◽  
C. E. Sekeris ◽  
P. Karlson
Keyword(s):  
Juvenile Hormone ◽  
Dna Hybridization ◽  
Rna Synthesis ◽  
Fat Body ◽  
Specific Effect ◽  
Antagonistic Effect ◽  
Hormone Action ◽  
Cell Nuclei ◽  
Sedimentation Pattern ◽  
Body Cell

The RNA synthesized in isolated fat body cell nuclei of blowfly larvae under the influence of ecdysone and juvenile hormone was studied by sucrose density centrifugation and RNA-DNA-hybridization.RNA produced in isolated nuclei of larvae in the early third instar has a polydisperse sedimentation pattern, showing a maximum at about 14 S, while RNA synthesized in nuclei of larvae in a later developmental stage has, in average, lower sedimentation coefficients. No significant differences in sedimentation properties could be seen between RNA of control and hormone-treated nuclei.An investigation of the hybridization of RNA synthesized in hormone-treated nuclei showed that the RNA produced under the influence of ecdysone or juvenile hormone is qualitatively different from that of the controls. This points to a specific effect of the hormones on the genome. The antagonistic effect of ecdysone and juvenile hormone on RNA synthesis is discussed on the basis of hybridization studies.

Increased juvenile hormone levels after long-duration flight in the grasshopper, Melanoplus sanguinipes

2004 ◽  
Vol 50 (6) ◽  
pp. 531-537 ◽  
Author(s):  
Kyung Jin Min ◽  
Nathan Jones ◽  
David W. Borst ◽  
Mary Ann Rankin
Keyword(s):  
Juvenile Hormone ◽  
Melanoplus Sanguinipes ◽  
Hormone Levels ◽  
Long Duration

Effect of Some Metabolic Inhibitors on Vinblastine-Induced Ribosomal-Granular Material Complexes

1971 ◽  
Vol 29 ◽  
pp. 548-549
Author(s):  
Awtar Krishan ◽  
Dora Hsu
Keyword(s):  
Granular Material ◽  
Rna Synthesis ◽  
Culture Medium ◽  
Actinomycin D ◽  
Metabolic Inhibitors ◽  
Protein And Rna Synthesis ◽  
Apparent Reduction ◽  
Plant Alkaloids ◽  
In Cells

Cells exposed to antitumor plant alkaloids, vinblastine and vincristine sulfate have large proteinacious crystals and complexes of ribosomes, helical polyribosomes and electron-dense granular material (ribosomal complexes) in their cytoplasm, Binding of H3-colchicine by the in vivo crystals shows that they contain microtubular proteins. Association of ribosomal complexes with the crystals suggests that these structures may be interrelated.In the present study cultured human leukemic lymphoblasts (CCRF-CEM), were incubated with protein and RNA-synthesis inhibitors, p. fluorophenylalanine, puromycin, cycloheximide or actinomycin-D before the addition of crystal-inducing doses of vinblastine to the culture medium. None of these compounds could completely prevent the formation of the ribosomal complexes or the crystals. However, in cells pre-incubated with puromycin, cycloheximide, or actinomycin-D, a reduction in the number and size of the ribosomal complexes was seen. Large helical polyribosomes were absent in the ribosomal complexes of cells treated with puromycin, while in cells exposed to cycloheximide, there was an apparent reduction in the number of ribosomes associated with the ribosomal complexes (Fig. 2).

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