Preliminary Evidence Suggesting Variations of GtH 1 and GtH 2 mRNA Levels at Different Stages of Gonadal Development in Rainbow Trout, Oncorhynchus mykiss

1995 ◽  
Vol 100 (3) ◽  
pp. 327-333 ◽  
Author(s):  
C. Weil ◽  
M. Bougoussa-Houadec ◽  
C. Gallais ◽  
S. Itoh ◽  
S. Sekine ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Gonadal Development ◽  
Preliminary Evidence ◽  
Mrna Levels ◽  
Rainbow Trout Oncorhynchus Mykiss

Differential expression of corticosteroid receptor genes in rainbow trout (Oncorhynchus mykiss) immune system in response to acute stress

2007 ◽  
Vol 64 (10) ◽  
pp. 1382-1389 ◽  
Author(s):  
Takashi Yada ◽  
Teruo Azuma ◽  
Susumu Hyodo ◽  
Tetsuya Hirano ◽  
E Gordon Grau ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Plasma Cortisol ◽  
Glucocorticoid Receptors ◽  
Acute Stress ◽  
Mrna Levels ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Corticosteroid Receptor ◽  
Polymerase Chain ◽  
Peripheral Blood Leucocytes

Expression of distinct corticosteroid receptor genes, glucocorticoid receptors 1 and 2 (GR-1 and GR-2, respectively) and mineralcorticoid receptor (MR), was quantified by real-time polymerase chain reaction (PCR) in peripheral blood leucocytes (PBL), spleen, and gill of rainbow trout (Oncorhynchus mykiss) after an acute netting stress. Plasma cortisol levels were significantly increased 2 h after stress and returned to prestress levels within 24 h. Consistent with changes in plasma cortisol, GR-2 mRNA levels in PBL increased significantly at 2 h after stress, returning to initial levels by 8 h. In contrast, GR-1 and MR levels in PBL decreased significantly at 24 h after stress, and these reduced levels were maintained for 7 days. Splenic mRNA levels of GR-1 and GR-2 also decreased at 8 h and 24 h after stress, returning to control levels by 7 days, whereas no significant change was observed in MR. In gill, there was no obvious change in corticosteroid receptor mRNA levels after stress, except for a transient decrease at 8 h in MR. These results suggest a variety of roles for the three corticosteroid receptors during immunosuppression in response to acute stress in trout.

Enhanced growth and retarded gonadal development of farmed rainbow trout,Oncorhynchus mykiss(Walbaum) following a long-day photoperiod

2014 ◽  
Vol 46 (10) ◽  
pp. 2398-2406 ◽  
Author(s):  
Ahmad Noori ◽  
Bagher Mojazi Amiri ◽  
Alireza Mirvaghefi ◽  
Gholamreza Rafiee ◽  
Bita Kalvani Neitali
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Gonadal Development ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Long Day

Expression of cytochrome genes during the early gonadal development in triploid female rainbow trout Oncorhynchus mykiss

2018 ◽  
Vol 25 (5) ◽  
pp. 949
Author(s):  
Fusheng XIANG ◽  
Gefeng XU ◽  
Wei GU ◽  
Tianqing HUANG ◽  
Chenbin LIU ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Gonadal Development ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Triploid Female

scholarly journals Molecular cloning and expression analysis of rainbow trout (Oncorhynchus mykiss) CCAAT/enhancer binding protein genes and their responses to induction by GH in vitro and in vivo

2007 ◽  
Vol 194 (2) ◽  
pp. 393-406 ◽  
Author(s):  
Jay H Lo ◽  
Pinwen Peter Chiou ◽  
C M Lin ◽  
Thomas T Chen
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Cloning And Expression ◽  
Mrna Levels ◽  
Specific Expression ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Enhancer Binding Protein ◽  
Previous Hypothesis

CCAAT/enhancer-binding proteins (C/EBPs) are transcription factors consisting of six isoforms and play diverse physiological roles in vertebrates. In rainbow trout (Oncorhynchus mykiss), in addition to the reported C/EBPβ1, we have isolated cDNA of four other isoforms, C/EBPα, C/EBPβ2, C/EBPδ1, C/EBPδ2, from the liver. Comparison of the deduced amino acid sequence of rainbow trout C/EBPs with those of other vertebrates revealed that C/EBP isoforms are highly conserved. The profiles of tissue-specific expression of individual C/EBP isoform mRNA, determined by quantitative real-time (RT)-PCR showed distinct patterns. Furthermore, injection of bovine GH into yearling rainbow trout resulted in a significant increase of mRNA levels of C/EBPβ1, C/EBPβ2, and C/EBPδ2 but not C/EBPα and C/EBPδ1 in the liver. GH-dependent increase of mRNA levels of C/EBPβ1, C/EBPβ2, C/EBPδ2, and IGF-II were also confirmed by treating rainbow trout hepatoma cells expressing a goldfish GH receptor with bGH. Together with our previous findings, the results presented in this paper strengthen our previous hypothesis that GH may regulate the expression of the IGF-II gene via mediating the expression of C/EBPβ1, C/EBPβ2, and C/EBPδ2 mRNA.

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