Effects of KATP Channel Openers, P-1075, Pinacidil, and Diazoxide, on Energetics and Contractile Function in Isolated Rat Hearts

2002 ◽  
Vol 34 (4) ◽  
pp. 427-440 ◽  
Author(s):  
Olga Jilkina ◽  
Bozena Kuzio ◽  
Gary J. Grover ◽  
Valery V. Kupriyanov
Keyword(s):  
Katp Channel ◽  
Contractile Function ◽  
Rat Hearts ◽  
Isolated Rat ◽  
Katp Channel Openers

Ruthenium Red Improves Postischemic Contractile Function in Isolated Rat Hearts

1990 ◽  
Vol 16 (5) ◽  
pp. 783-789 ◽  
Author(s):  
Gary J. Grover ◽  
Steven Dzwonczyk ◽  
Paul G. Sleph
Keyword(s):  
Contractile Function ◽  
Ruthenium Red ◽  
Rat Hearts ◽  
Isolated Rat

scholarly journals Preconditioning in rat hearts is independent of mitochondrial F1F0ATPase inhibition

1998 ◽  
Vol 274 (1) ◽  
pp. H90-H97 ◽  
Author(s):  
David W. Green ◽  
Holt N. Murray ◽  
Paul G. Sleph ◽  
Feng-Lai Wang ◽  
Anne J. Baird ◽  
...  
Keyword(s):  
Atp Hydrolysis ◽  
Contractile Function ◽  
Atp Synthesis ◽  
Isolated Rat Heart ◽  
Global Ischemia ◽  
Mitochondrial Atpase ◽  
Atpase Inhibitor ◽  
Lactate Dehydrogenase Release ◽  
Rat Hearts ◽  
Isolated Rat

Mitochondrial F1F0adenosinetriphosphatase (ATPase) is responsible for the majority of ATP synthesis during normoxic conditions, but under ischemic conditions it accounts for significant ATP hydrolysis. A previous study showed that preconditioning in isolated rat hearts is mediated by inhibition of this ATPase during ischemia. We tested this hypothesis in our isolated rat heart model of preconditioning. Preconditioning was accomplished by three 5-min periods of global ischemia separated by 5 min of reperfusion. This was followed by 20 min of global ischemia and 30 min of reperfusion. Preconditioning significantly enhanced reperfusion contractile function and reduced lactate dehydrogenase release but paradoxically reduced the time to onset of contracture during global ischemia. Myocardial ATP was depleted at a faster rate during the prolonged ischemia in preconditioned than in sham-treated hearts, which is consistent with the reduced time to contracture. ATP during reperfusion was repleted more rapidly in preconditioned hearts, which is consistent with their enhanced contractile function. Preconditioning significantly reduced lactate accumulation during the prolonged ischemia. We were not able to demonstrate that mitochondrial F1F0ATPase (measured in submitochondrial particles) was inhibited by preconditioning before or during the prolonged ischemia. The mitochondrial ATPase inhibitor oligomycin significantly conserved ATP during ischemia and increased the time to the onset of contracture, which is consistent with inhibition of the mitochondrial ATPase. Our results show that preconditioning in rat hearts can be independent of mitochondrial ATPase inhibition as well as ATP conservation.

scholarly journals Polyphenol (-)-Epigallocatechin Gallate during Ischemia Limits Infarct Size Via Mitochondrial KATP Channel Activation in Isolated Rat Hearts

2010 ◽  
Vol 25 (3) ◽  
pp. 380 ◽  
Author(s):  
Dae-Kyu Song ◽  
Youngho Jang ◽  
June Hong Kim ◽  
Kook-Jin Chun ◽  
Deokhee Lee ◽  
...  
Keyword(s):  
Infarct Size ◽  
Katp Channel ◽  
Epigallocatechin Gallate ◽  
Channel Activation ◽  
Mitochondrial Katp Channel ◽  
Rat Hearts ◽  
Isolated Rat

Alpha lipoic acid protects the heart against myocardial post ischemia–reperfusion arrhythmias via KATP channel activation in isolated rat hearts

2014 ◽  
Vol 66 (3) ◽  
pp. 499-504 ◽  
Author(s):  
Magdalena Dudek ◽  
Joanna Knutelska ◽  
Marek Bednarski ◽  
Leszek Nowiński ◽  
Małgorzata Zygmunt ◽  
...  
Keyword(s):  
Lipoic Acid ◽  
Katp Channel ◽  
Ischemia Reperfusion ◽  
Alpha Lipoic Acid ◽  
Reperfusion Arrhythmias ◽  
Channel Activation ◽  
Rat Hearts ◽  
Isolated Rat

Low-density lipoproteins inhibit histamine and NaNO2 relaxations of the coronary vasculature and reduce contractile function in isolated rat hearts

1995 ◽  
Vol 10 (5) ◽  
pp. 249-257 ◽  
Author(s):  
Glenn J. Harrison ◽  
Lindsay R. Jordan ◽  
Michael L. Selley ◽  
Roger J. Willis
Keyword(s):  
Contractile Function ◽  
Low Density Lipoproteins ◽  
Low Density ◽  
Coronary Vasculature ◽  
Rat Hearts ◽  
Isolated Rat

Design, Synthesis and Biological Evaluation of Benzopyran Derivatives as KATP Channel Openers

2010 ◽  
Vol 7 (6) ◽  
pp. 415-420 ◽  
Author(s):  
Jinpei Zhou ◽  
Hai Qian ◽  
Huibin Zhang ◽  
Hui Gao ◽  
Wenlong Huang ◽  
...  
Keyword(s):  
Katp Channel ◽  
Biological Evaluation ◽  
Design Synthesis ◽  
Synthesis And Biological Evaluation ◽  
Katp Channel Openers

Independent dual perfusion of left and right coronary arteries in isolated rat hearts

1991 ◽  
Vol 261 (6) ◽  
pp. H2082-H2090 ◽  
Author(s):  
M. Avkiran ◽  
M. J. Curtis
Keyword(s):  
High Energy ◽  
Low Flow ◽  
Blue Dye ◽  
Ischemia And Reperfusion ◽  
Langendorff Preparation ◽  
Rat Hearts ◽  
Left And Right ◽  
Conventional Models ◽  
Aortic Cannula ◽  
Isolated Rat

A novel dual lumen aortic cannula was designed and constructed to permit independent perfusion of left and right coronary beds in isolated rat hearts without necessitating the cannulation of individual arteries. Stability of the dual-perfusion preparation was shown to be similar to that of the conventional Langendorff preparation, in terms of coronary flow, heart rate, and high-energy phosphate content. The independence of left and right perfusion beds was confirmed by unilateral infusion of disulfine blue dye and spectrophotometric detection of the dye in ventricular homogenates. Transient cessation of flow to the left coronary bed resulted in severe ventricular arrhythmias upon reperfusion, as in conventional models of regional ischemia and reperfusion. The dual-perfusion model is technically undemanding, reproducible, inexpensive, and can be used in several species. It enables studies with 1) regional low flow ischemia, 2) regional zero-flow ischemia without coronary ligation (with attendant damage to vasculature), 3) selective application of drugs or interventions to the ischemic-reperfused zone, and 4) selective application of components of ischemia and reperfusion to a site anatomically relevant to ischemic heart disease.

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