Staphylococcus aureus α-toxin and Escherichia coli hemolysin impair cardiac regional perfusion and contractile function by activating myocardial eicosanoid metabolism in isolated rat hearts

2009 ◽  
Vol 37 (6) ◽  
pp. 2025-2032 ◽  
Author(s):  
Ulrich Grandel ◽  
Ulrich Bennemann ◽  
Michael Buerke ◽  
Katja Hattar ◽  
Werner Seeger ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Contractile Function ◽  
Regional Perfusion ◽  
Rat Hearts ◽  
Eicosanoid Metabolism ◽  
Escherichia Coli Hemolysin ◽  
Isolated Rat

Ruthenium Red Improves Postischemic Contractile Function in Isolated Rat Hearts

1990 ◽  
Vol 16 (5) ◽  
pp. 783-789 ◽  
Author(s):  
Gary J. Grover ◽  
Steven Dzwonczyk ◽  
Paul G. Sleph
Keyword(s):  
Contractile Function ◽  
Ruthenium Red ◽  
Rat Hearts ◽  
Isolated Rat

scholarly journals Preconditioning in rat hearts is independent of mitochondrial F1F0ATPase inhibition

1998 ◽  
Vol 274 (1) ◽  
pp. H90-H97 ◽  
Author(s):  
David W. Green ◽  
Holt N. Murray ◽  
Paul G. Sleph ◽  
Feng-Lai Wang ◽  
Anne J. Baird ◽  
...  
Keyword(s):  
Atp Hydrolysis ◽  
Contractile Function ◽  
Atp Synthesis ◽  
Isolated Rat Heart ◽  
Global Ischemia ◽  
Mitochondrial Atpase ◽  
Atpase Inhibitor ◽  
Lactate Dehydrogenase Release ◽  
Rat Hearts ◽  
Isolated Rat

Mitochondrial F1F0adenosinetriphosphatase (ATPase) is responsible for the majority of ATP synthesis during normoxic conditions, but under ischemic conditions it accounts for significant ATP hydrolysis. A previous study showed that preconditioning in isolated rat hearts is mediated by inhibition of this ATPase during ischemia. We tested this hypothesis in our isolated rat heart model of preconditioning. Preconditioning was accomplished by three 5-min periods of global ischemia separated by 5 min of reperfusion. This was followed by 20 min of global ischemia and 30 min of reperfusion. Preconditioning significantly enhanced reperfusion contractile function and reduced lactate dehydrogenase release but paradoxically reduced the time to onset of contracture during global ischemia. Myocardial ATP was depleted at a faster rate during the prolonged ischemia in preconditioned than in sham-treated hearts, which is consistent with the reduced time to contracture. ATP during reperfusion was repleted more rapidly in preconditioned hearts, which is consistent with their enhanced contractile function. Preconditioning significantly reduced lactate accumulation during the prolonged ischemia. We were not able to demonstrate that mitochondrial F1F0ATPase (measured in submitochondrial particles) was inhibited by preconditioning before or during the prolonged ischemia. The mitochondrial ATPase inhibitor oligomycin significantly conserved ATP during ischemia and increased the time to the onset of contracture, which is consistent with inhibition of the mitochondrial ATPase. Our results show that preconditioning in rat hearts can be independent of mitochondrial ATPase inhibition as well as ATP conservation.

Antimicrobial and cytotoxic activity of Macrophomina phaseolina isolated from gummosis infected citrus reticulata

2013 ◽  
pp. 125-133
Author(s):  
Rubal C Das ◽  
Rajib Banik ◽  
Robiul Hasan Bhuiyan ◽  
Md Golam Kabir
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Brine Shrimp ◽  
Inhibitory Concentration ◽  
Lethal Dose ◽  
Macrophomina Phaseolina ◽  
Chloroform Extract ◽  
Citrus Reticulata ◽  
Gram Negative Bacteria ◽  
Gram Negative

Macrophomina phaseolina is one of the pathogenic organisms of gummosis disease of orange tree (Citrus reticulata). The pathogen was identified from the observation of their colony size, shape, colour, mycelium, conidiophore, conidia, hyaline, spore, and appressoria in the PDA culture. The crude chloroform extracts from the organism showed antibacterial activity against a number of Gram positive and Gram-negative bacteria. The crude chloroform extract also showed promising antifungal activity against three species of the genus Aspergillus. The minimum inhibitory concentration (MIC) of the crude chloroform extract from M. phaseolina against Bacillus subtilis, Staphylococcus aureus, Escherichia coli, and Shigella sonnie were 128 ?gm, 256 ?gm, 128 ?gm and 64 ?gm/ml respectively. The LD50 (lethal dose) values of the cytotoxicity assay over brine shrimp of the crude chloroform extract from M. phaseolina was found to be 51.79 ?gm/ml. DOI: http://dx.doi.org/10.3329/cujbs.v5i1.13378 The Chittagong Univ. J. B. Sci.,Vol. 5(1 &2):125-133, 2010

Screening and antimicrobial activity of actinomycetes isolated from the rhizosphere of Clitoria ternatea

2016 ◽  
Vol 1 (01) ◽  
Author(s):  
Vemavarapu Bhaskara Rao ◽  
Kandlagunta Guru Prasad ◽  
Krishna Naragani ◽  
Vijayalakshmi Muvva
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Calcium Carbonate ◽  
Rhizosphere Soil ◽  
Soil Samples ◽  
Antimicrobial Spectrum ◽  
Clitoria Ternatea ◽  
Primary Screening

The air dried rhizosphere soil samples pretreated with calcium carbonate was employed for the isolation of actinomycete strains. Serial dilution plate technique was used for the isolation of actinomycetes. A total of 20 actinomycete strains designated as BS1-BS20 were isolated from the rhizosphere of medicinal plant Clitoria ternatea. All the 20 strains were subjected to primary screening for antimicrobial activity. Among the 20 strains screened, 10 strains exhibited high antimicrobial spectrum against Staphylococcus aureus, Escherichia coli and Candida albicans.

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