A Structural Study of Isolated Mammalian Centrioles Using Negative Staining Electron Microscopy

1996 ◽  
Vol 117 (3) ◽  
pp. 222-226 ◽  
Author(s):  
Bodo M.H. Lange ◽  
Keith Gull
Keyword(s):  
Electron Microscopy ◽  
Structural Study ◽  
Negative Staining ◽  
Negative Staining Electron Microscopy

scholarly journals Appearance of the Bona Fide Spiral Tubule of Orf Virus Is Dependent on an Intact 10-Kilodalton Viral Protein

2004 ◽  
Vol 78 (15) ◽  
pp. 8085-8093 ◽  
Author(s):  
D. Spehner ◽  
S. De Carlo ◽  
R. Drillien ◽  
F. Weiland ◽  
K. Mildner ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Fusion Protein ◽  
Viral Protein ◽  
Mutant Virus ◽  
Negative Staining ◽  
Orf Virus ◽  
Gene Encoding ◽  
Virus Particles ◽  
Bona Fide ◽  
Negative Staining Electron Microscopy

ABSTRACT Parapoxviruses can be morphologically distinguished from other poxviruses in conventional negative staining electron microscopy (EM) by their ovoid appearance and the spiral tubule surrounding the virion's surface. However, this technique may introduce artifacts. We have examined Orf virus (ORFV; the prototype species of the Parapoxvirus genus) by cryoelectron microscopy (cryo-EM) and cryo-negative staining EM. From these studies we suggest that the shape and unique spiral tubule are authentic features of the parapoxviruses. We also constructed an ORFV mutant deleted of a gene encoding a 10-kDa protein, which is an orthologue of the vaccinia virus (VACV) 14-kDa fusion protein, and investigated its ultrastructure. This mutant virus multiplied slowly in permissive cells and produced infectious but morphologically aberrant particles. Mutant virions lacked the spiral tubule but displayed short disorganized tubules similar to those observed on the surface of VACV. In addition, thin extensions or loop-like structures were appended to the ORFV mutant particles. We suggest that these appended structures arise from a failure of the mutant virus particles to properly seal and that the sealing activity is dependent on the 10-kDa protein.

Negative-staining Electron Microscopy of the Urine for the Detection of Polyomavirus Infections

2006 ◽  
Vol 30 (5) ◽  
pp. 329-338 ◽  
Author(s):  
Harsharan K. Singh ◽  
Victoria Madden ◽  
You Jun Shen ◽  
Bawana D. Thompson ◽  
Volker Nickeleit
Keyword(s):  
Electron Microscopy ◽  
Negative Staining ◽  
Negative Staining Electron Microscopy
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