Sequencing and Characterization of the Cryptic Plasmid QpRS from Coxiella burnetii

Plasmid ◽  
2000 ◽  
Vol 44 (1) ◽  
pp. 85-88 ◽  
Author(s):  
S. Lautenschläger ◽  
H. Willems ◽  
C. Jäger ◽  
G. Baljer
Keyword(s):  
Coxiella Burnetii ◽  
Cryptic Plasmid

scholarly journals First molecular detection and genetic characterization of Coxiella burnetii in Zambian dogs and rodents

2018 ◽  
Vol 11 (1) ◽  
Author(s):  
Simbarashe Chitanga ◽  
Edgar Simulundu ◽  
Martin C. Simuunza ◽  
Katendi Changula ◽  
Yongjin Qiu ◽  
...  
Keyword(s):  
Coxiella Burnetii ◽  
Molecular Detection ◽  
Genetic Characterization

scholarly journals Isolation and characterization of a plasmid from phase I Coxiella burnetii.

1983 ◽  
Vol 41 (2) ◽  
pp. 488-493 ◽  
Author(s):  
J E Samuel ◽  
M E Frazier ◽  
M L Kahn ◽  
L S Thomashow ◽  
L P Mallavia
Keyword(s):  
Phase I ◽  
Coxiella Burnetii ◽  
Isolation And Characterization

Characterization of a Cryptic Plasmid, pSM103mini, from Polyethylene-Glycol DegradingSphingopyxis macrogoltabidaStrain 103

2011 ◽  
Vol 75 (2) ◽  
pp. 295-298 ◽  
Author(s):  
Akio TANI ◽  
Akiyuki TANAKA ◽  
Toshiyuki MINAMI ◽  
Kazuhide KIMBARA ◽  
Fusako KAWAI
Keyword(s):  
Polyethylene Glycol ◽  
Cryptic Plasmid

Characterization of the cryptic plasmid pBGR1 from Bartonella grahamii and construction of a versatile Escherichia coli–Bartonella spp. shuttle cloning vector

Plasmid ◽  
2003 ◽  
Vol 49 (1) ◽  
pp. 44-52 ◽  
Author(s):  
Anja Seubert ◽  
Christine Falch ◽  
Richard J Birtles ◽  
Ralf Schulein ◽  
Christoph Dehio
Keyword(s):  
Escherichia Coli ◽  
Cloning Vector ◽  
Cryptic Plasmid

Characterization of a cryptic plasmid from endophytic Enterobacter agglomerans isolated from Amazon’s Copaifera multijuga

2015 ◽  
Vol 4 (1) ◽  
pp. 67 ◽  
Author(s):  
Alessandra Karisa Costa Lima do Nascimento ◽  
Lucivana Prata Souza Mourão ◽  
Enedina Nogueira de Assunção ◽  
Carlos Gustavo Nunes-Silva ◽  
Edmar Vaz de Andrade ◽  
...  
Keyword(s):  
Cryptic Plasmid ◽  
Enterobacter Agglomerans

Nucleotide sequence of the cryptic plasmid pTT8 from Thermus thermophilus HB8 and isolation and characterization of its high-copy-number mutant

Plasmid ◽  
2004 ◽  
Vol 51 (3) ◽  
pp. 227-237 ◽  
Author(s):  
Goh Takayama ◽  
Takehide Kosuge ◽  
Hideaki Maseda ◽  
Akira Nakamura ◽  
Takayuki Hoshino
Keyword(s):  
Nucleotide Sequence ◽  
Copy Number ◽  
Thermus Thermophilus ◽  
Cryptic Plasmid ◽  
High Copy Number ◽  
Thermus Thermophilus Hb8 ◽  
Isolation And Characterization

scholarly journals Serological characterization of surface-exposed proteins of Coxiella burnetii

Microbiology ◽  
2014 ◽  
Vol 160 (12) ◽  
pp. 2718-2731 ◽  
Author(s):  
Jun Jiao ◽  
Xiaolu Xiong ◽  
Yong Qi ◽  
Wenping Gong ◽  
Changsong Duan ◽  
...  
Keyword(s):  
Coxiella Burnetii ◽  
Bioinformatics Analysis ◽  
Q Fever ◽  
Obligate Intracellular ◽  
Rickettsia Rickettsii ◽  
Serological Characterization ◽  
Vaccine Antigens ◽  
Mycoplasma Pneumonia ◽  
Dimensional Electrophoresis

The obligate intracellular Gram-negative bacterium Coxiella burnetii causes Q fever, a worldwide zoonosis. Here we labelled Cox . burnetii with biotin and used biotin-streptavidin affinity chromatography to isolate surface-exposed proteins (SEPs). Using two-dimensional electrophoresis combined with mass spectrometry, we identified 37 proteins through bioinformatics analysis. Thirty SEPs expressed in Escherichia coli (recombinant SEPs, rSEPs) were used to generate microarrays, which were probed with sera from mice experimentally infected with Cox. burnetii or sera from Q fever patients. Thirteen rSEPs were recognized as seroreactive, and the majority reacted with at least 50 % of the sera from mice infected with Cox. burnetii but not with sera from mice infected with Rickettsia rickettsii, R. heilongjiangensis, or R. typhi. Further, 13 proteins that reacted with sera from patients with Q fever did not react with sera from patients with brucellosis or mycoplasma pneumonia. Our results suggest that these seroreactive SEPs have potential as serodiagnostic antigens or as subunit vaccine antigens against Q fever.

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