A P Element Transformation Vector for High Levels of Gene Expression in Germ-Line Cells of the Ovary and Undifferentiated Cells in the Developing Eye of Drosophila

Plasmid ◽  
2002 ◽  
Vol 47 (1) ◽  
pp. 61-65 ◽  
Author(s):  
Xin Chen ◽  
Janice A. Fischer
Keyword(s):  
Gene Expression ◽  
Germ Line ◽  
P Element ◽  
Transformation Vector ◽  
Undifferentiated Cells ◽  
P Element Transformation

scholarly journals Can random mutation explain phenotypic variability?

2018 ◽  
Author(s):  
Víctor Alejandro Zapata Trejo
Keyword(s):  
Gene Expression ◽  
Somatic Cell ◽  
Germ Line ◽  
Phenotypic Variability ◽  
Random Mutation ◽  
Differentiated Cells ◽  
Undifferentiated Cells ◽  
Joint Evolution

The epigenome regulates the gene expression of all differentiated cells and indicates which specific genes must be transcribed. It is argued that the expression factors that act on specific genes of the somatic cell involved in a behavior also act on the transcription of the same genes in the most undifferentiated cells of the germ line. It is proposed how a probabilistic view of the random mutation can explain the evolution of the phenotypes and integrate all the evidence pointing to a joint evolution with the environment.

scholarly journals Can random mutation explain the phenotypic variability?

2018 ◽  
Author(s):  
Víctor Alejandro Zapata Trejo
Keyword(s):  
Gene Expression ◽  
Somatic Cell ◽  
Germ Line ◽  
Phenotypic Variability ◽  
Random Mutation ◽  
Differentiated Cells ◽  
Undifferentiated Cells

The epigenome regulates the gene expression of all differentiated cells and indicates which specific genes must be transcribed. It is argued that the expression factors that act on specific genes of the somatic cell involved in a behavior also act on the transcription of the same genes in the most undifferentiated cells of the germ line. It is proposed how a probabilistic view of the random mutation can explain the evolution of the phenotypes and integrate all the evidence pointing to a conjunct evolution with the environment.

scholarly journals Hypothesis of the conjunct expression gene: can random mutation explain the phenotypic variability?

2018 ◽  
Author(s):  
Víctor A Zapata Trejo
Keyword(s):  
Gene Expression ◽  
Germ Line ◽  
Phenotypic Variability ◽  
Somatic Cells ◽  
Random Mutation ◽  
Differentiated Cells ◽  
Undifferentiated Cells

The epigenome regulates the gene expression of all differentiated cells and indicates which specific genes must be transcribed. It is argued that the expression factors that act in specific genes of the somatic cells involved in a behavior also act in the partial transcription of the same genes in the most undifferentiated cells of the germ line. It is proposed how a probabilistic view of the random mutation can explain the evolution of the phenotypes and integrate all the evidence pointing to a conjunct evolution with the environment.

scholarly journals Hypothesis of the conjunct expression gene: can random mutation explain the phenotypic variability?

2018 ◽  
Author(s):  
Víctor A Zapata Trejo
Keyword(s):  
Gene Expression ◽  
Natural Selection ◽  
Functional Role ◽  
Germ Line ◽  
Phenotypic Variability ◽  
Point Of View ◽  
Random Mutation ◽  
Undifferentiated Cells ◽  
Gene Expression Levels

If the environmental conditions can or not influence in the phenotype evolution, is a topic that has been discussed during the last ten years. The epigenome regulates the gene expression of all cells and indicates which specific genes must be transcribed. It is argued that the expression factors that act in specific genes of the somatic cells involved in a behavior also act in the partial transcription of the same genes in the most undifferentiated cells of the germ line. The functional role of the isochores as an epigenetic determinant regulator of the transcription and therefore of gene expression levels is discussed. It is proposed how a probabilistic view of the random mutation can integrate all the evidence pointing to a conjunct phenotype evolution with the environment. This work provides a new point of view on how the environment can influence in adaptation through natural selection considering the theory currently accepted.

scholarly journals Hypothesis of the conjunct expression gene: can random mutation explain the phenotypic variability?

2018 ◽  
Author(s):  
Víctor A Zapata Trejo
Keyword(s):  
Gene Expression ◽  
Natural Selection ◽  
Functional Role ◽  
Germ Line ◽  
Phenotypic Variability ◽  
Point Of View ◽  
Random Mutation ◽  
Undifferentiated Cells ◽  
Gene Expression Levels

If the environmental conditions can or not influence in the phenotype evolution, is a topic that has been discussed during the last ten years. The epigenome regulates the gene expression of all cells and indicates which specific genes must be transcribed. It is argued that the expression factors that act in specific genes of the somatic cells involved in a behavior also act in the partial transcription of the same genes in the most undifferentiated cells of the germ line. The functional role of the isochores as an epigenetic determinant regulator of the transcription and therefore of gene expression levels is discussed. It is proposed how a probabilistic view of the random mutation can integrate all the evidence pointing to a conjunct phenotype evolution with the environment. This work provides a new point of view on how the environment can influence in adaptation through natural selection considering the theory currently accepted.

Distinct expression patterns detected within individual tissues by the GAL4 enhancer trap technique

Genome ◽  
1996 ◽  
Vol 39 (1) ◽  
pp. 174-182 ◽  
Author(s):  
Kerstin Gustafson ◽  
Gabrielle L. Boulianne
Keyword(s):  
Gene Expression ◽  
Transcriptional Control ◽  
Germ Line ◽  
Expression Patterns ◽  
Cell Types ◽  
Enhancer Trap ◽  
P Element ◽  
Immunocytochemical Staining ◽  
Specific Cell ◽  
Drosophila Genome

To identify genes that are expressed in specific cell types or tissues during development, we generated enhancer-trap lines in which the yeast transcriptional activator, GAL4, was mobilized throughout the Drosophila genome. The GAL4 lines are part of a two-part system involving GAL4 and its target, the upstream activating sequence (UAS). Detection of GAL4 expression patterns was achieved by crossing individual GAL4 lines with flies carrying the reporter gene lacZ under the transcriptional control of the UAS followed by histochemical and immunocytochemical staining. Here, we present the results of this screen and the characterization of GAL4 lines that show distinct patterns of gene expression during Drosophila development, including embryogenesis, oogenesis, and imaginai disc development. However, we were unable to identify GAL4 lines that were expressed within the germ line or during early embryogenesis. Furthermore, consistent with previous results, we found that the GAL4 enhancer trap technique had a much lower frequency of transposition than has been reported for lacZ enhancer trap screens. Taken together, these results demonstrate both the strengths and weaknesses of the GAL4 enhancer trap technique for identifying unique patterns of gene expression during development. Key words : GAL4, enhancer trap, Drosophila, P element.

scholarly journals Can random mutation explain the phenotypic variability?

2018 ◽  
Author(s):  
Víctor A Zapata Trejo
Keyword(s):  
Gene Expression ◽  
Germ Line ◽  
Phenotypic Variability ◽  
Somatic Cells ◽  
Random Mutation ◽  
Differentiated Cells ◽  
Undifferentiated Cells

The epigenome regulates the gene expression of all differentiated cells and indicates which specific genes must be transcribed. It is argued that the expression factors that act in specific genes of the somatic cells involved in a behavior also act in the partial transcription of the same genes in the most undifferentiated cells of the germ line. It is proposed how a probabilistic view of the random mutation can explain the evolution of the phenotypes and integrate all the evidence pointing to a conjunct evolution with the environment.

scholarly journals Hypothesis of the conjunct expression gene: can random mutation explain the phenotypic variability?

2018 ◽  
Author(s):  
Víctor A Zapata Trejo
Keyword(s):  
Gene Expression ◽  
Natural Selection ◽  
Functional Role ◽  
Germ Line ◽  
Phenotypic Variability ◽  
Point Of View ◽  
Random Mutation ◽  
Undifferentiated Cells ◽  
Gene Expression Levels

If the environmental conditions can or not influence in the phenotype evolution, is a topic that has been discussed during the last ten years. The epigenome regulates the gene expression of all cells and indicates which specific genes must be transcribed. It is argued that the expression factors that act in specific genes of the somatic cells involved in a behavior also act in the partial transcription of the same genes in the most undifferentiated cells of the germ line. The functional role of the isochores as an epigenetic determinant regulator of the transcription and therefore of gene expression levels is discussed. It is proposed how a probabilistic view of the random mutation can integrate all the evidence pointing to a conjunct phenotype evolution with the environment. This work provides a new point of view on how the environment can influence in adaptation through natural selection considering the theory currently accepted.

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