Faculty Opinions recommendation of Transformation vector based on promoter and intron sequences of a replacement histone H3 gene. A tool for high, constitutive gene expression in plants.

2002 ◽  
Author(s):  
Steven Spiker
Keyword(s):  
Gene Expression ◽  
Histone H3 ◽  
Transformation Vector ◽  
Constitutive Gene Expression ◽  
Histone H3 Gene

Transformation vector based on promoter and intron sequences of a replacement histone H3 gene. A tool for high, constitutive gene expression in plants

2002 ◽  
Vol 11 (1) ◽  
pp. 69-72 ◽  
Author(s):  
Zsolt Kelemen ◽  
Antal Mai ◽  
Tamás Kapros ◽  
Attila Fehér ◽  
János Györgyey ◽  
...  
Keyword(s):  
Gene Expression ◽  
Histone H3 ◽  
Transformation Vector ◽  
Constitutive Gene Expression ◽  
Histone H3 Gene

scholarly journals Isolation and Use of a Homologous Histone H4 Promoter and a Ribosomal DNA Region in a Transformation Vector for the Oil-Producing Fungus Mortierella alpina

2000 ◽  
Vol 66 (11) ◽  
pp. 4655-4661 ◽  
Author(s):  
Donald A. Mackenzie ◽  
Prasert Wongwathanarat ◽  
Andrew T. Carter ◽  
David B. Archer
Keyword(s):  
Ribosomal Dna ◽  
Histone H3 ◽  
Mortierella Alpina ◽  
Hygromycin B ◽  
Chromosomal Integration ◽  
Histone H4 ◽  
Reading Frame ◽  
Transformation Vector ◽  
Histone H3 Gene ◽  
Drive Gene

ABSTRACT Mortierella alpina was transformed successfully to hygromycin B resistance by using a homologous histone H4 promoter to drive gene expression and a homologous ribosomal DNA region to promote chromosomal integration. This is the first description of transformation in this commercially important oleaginous organism. Two pairs of histone H3 and H4 genes were isolated from this fungus. Each pair consisted of one histone H3 gene and one histone H4 gene, transcribed divergently from an intergenic promoter region. The pairs of encoded histone H3 or H4 proteins were identical in amino acid sequence. At the DNA level, each histone H3 or H4 open reading frame showed 97 to 99% identity to its counterpart but the noncoding regions had little sequence identity. Unlike the histone genes from other filamentous fungi, all four M. alpina genes lacked introns. During normal vegetative growth, transcripts from the two histone H4 genes were produced at approximately the same level, indicating that either histone H4 promoter could be used in transformation vectors. The generation of stable, hygromycin B-resistant transformants required the incorporation of a homologous ribosomal DNA region into the transformation vector to promote chromosomal integration.

Proximal promoter region of the wheat histone H3 gene confers S phase-specific gene expression in transformed rice cells

1993 ◽  
Vol 23 (3) ◽  
pp. 553-565 ◽  
Author(s):  
Norihiro Ohtsubo ◽  
Takuya Nakayama ◽  
Rie Terada ◽  
Ko Shimamoto ◽  
Masaki Iwabuchi
Keyword(s):  
Gene Expression ◽  
Promoter Region ◽  
Histone H3 ◽  
S Phase ◽  
Proximal Promoter ◽  
Specific Gene ◽  
Proximal Promoter Region ◽  
Specific Gene Expression ◽  
Histone H3 Gene

scholarly journals Proline Isomerization of Histone H3 Regulates Lysine Methylation and Gene Expression

Cell ◽  
2006 ◽  
Vol 126 (5) ◽  
pp. 905-916 ◽  
Author(s):  
Christopher J. Nelson ◽  
Helena Santos-Rosa ◽  
Tony Kouzarides
Keyword(s):  
Gene Expression ◽  
Histone H3 ◽  
Lysine Methylation ◽  
Proline Isomerization
Sign in / Sign up

Export Citation Format

Share Document