Comparison of the Nucleotide and Deduced Amino Acid Sequences of the Envelope Protein Genes of the Wild-Type French Viscerotropic Strain of Yellow Fever Virus and the Live Vaccine Strain, French Neurotropic Vaccine, Derived from It

Virology ◽  
1993 ◽  
Vol 192 (2) ◽  
pp. 692-695 ◽  
Author(s):  
A.D. Jennings ◽  
J.E. Whitby ◽  
P.D. Minor ◽  
A.D.T. Barrett
Keyword(s):  
Amino Acid ◽  
Yellow Fever ◽  
Vaccine Strain ◽  
Envelope Protein ◽  
Yellow Fever Virus ◽  
Live Vaccine ◽  
Amino Acid Sequences ◽  
Fever Virus ◽  
Live Vaccine Strain ◽  
Wild Type

scholarly journals Identification of envelope protein epitopes that are important in the attenuation process of wild-type yellow fever virus.

1992 ◽  
Vol 66 (7) ◽  
pp. 4265-4270 ◽  
Author(s):  
B K Sil ◽  
L M Dunster ◽  
T N Ledger ◽  
M R Wills ◽  
P D Minor ◽  
...  
Keyword(s):  
Yellow Fever ◽  
Envelope Protein ◽  
Yellow Fever Virus ◽  
Fever Virus ◽  
Wild Type

scholarly journals Yellow fever virus isolated from a fatal post vaccination event: an experimental comparative study with the 17DD vaccine strain in the Syrian hamster (Mesocricetus auratus)

2004 ◽  
Vol 37 (suppl 2) ◽  
pp. 69-74 ◽  
Author(s):  
Sueli Guerreiro Rodrigues ◽  
Amélia Paes de Andrade Travassos da Rosa ◽  
Ricardo Galler ◽  
Vera Lúcia Reis de Souza Barros ◽  
Conceição de Maria Almeida Vieira ◽  
...  
Keyword(s):  
Yellow Fever ◽  
Vaccine Strain ◽  
Yellow Fever Virus ◽  
Mesocricetus Auratus ◽  
Virus Antigen ◽  
Fever Virus ◽  
Wild Type ◽  
Post Vaccination ◽  
The Brain ◽  
Virus Strains

In order to investigate the pathogenicity of the virus strain GOI 4191 that was isolated from a fatal adverse event after yellow fever virus (YFV) vaccination, an experimental assay using hamsters (Mesocricetus auratus) as animal model and YFV 17DD vaccine strain as virus reference was accomplished. The two virus strains were inoculated by intracerebral, intrahepatic and subcutaneous routes. The levels of viremia, antibody response, and aminotransferases were determined in sera; while virus, antigen and histopathological changes were determined in the viscera. No viremia was detected for either strain following infection; the immune response was demonstrated to be more effective to strain GOI 4191; and no significant aminotransferase levels alterations were detected. Strain GOI 4191 was recovered only from the brain of animals inoculated by the IC route. Viral antigens were detected in liver and brain by immunohistochemical assay. Histothological changes in the viscera were characterized by inflammatory infiltrate, hepatocellular necrosis, and viral encephalitis. Histological alterations and detection of viral antigen were observed in the liver of animals inoculated by the intrahepatic route. These findings were similar for both strains used in the experiment; however, significant differences were observed from those results previously reported for wild type YFV strains.

scholarly journals Development of a Real-Time Reverse Transcription-PCR Assay for Global Differentiation of Yellow Fever Virus Vaccine-Related Adverse Events from Natural Infections

2018 ◽  
Vol 56 (6) ◽  
Author(s):  
Holly R. Hughes ◽  
Brandy J. Russell ◽  
Eric C. Mossel ◽  
John Kayiwa ◽  
Julius Lutwama ◽  
...  
Keyword(s):  
Adverse Events ◽  
Real Time ◽  
Yellow Fever ◽  
Vaccine Strain ◽  
Reverse Transcription ◽  
Yellow Fever Virus ◽  
Fever Virus ◽  
Wild Type ◽  
Pcr Assay ◽  
Reverse Transcription Pcr

ABSTRACT Yellow fever (YF) is a reemerging public health threat, with frequent outbreaks prompting large vaccination campaigns in regions of endemicity in Africa and South America. Specific detection of vaccine-related adverse events is resource-intensive, time-consuming, and difficult to achieve during an outbreak. To address this, we have developed a highly transferable rapid yellow fever virus (YFV) vaccine-specific real-time reverse transcription-PCR (RT-PCR) assay that distinguishes vaccine from wild-type lineages. The assay utilizes a specific hydrolysis probe that includes locked nucleic acids to enhance specific discrimination of the YFV17D vaccine strain genome. Promisingly, sensitivity and specificity analyses reveal this assay to be highly specific to vaccine strain(s) when tested on clinical samples and YFV cell culture isolates of global origin. Taken together, our data suggest the utility of this assay for use in laboratories of varied capacity for the identification and differentiation of vaccine-related adverse events from wild-type infections of both African and South American origin.

scholarly journals Infection of hepatocytes with 17-D vaccine-strain yellow fever virus induces a strong pro-inflammatory host response

2011 ◽  
Vol 92 (10) ◽  
pp. 2262-2271 ◽  
Author(s):  
Sara E. Woodson ◽  
Michael R. Holbrook
Keyword(s):  
Yellow Fever ◽  
Vaccine Strain ◽  
Host Response ◽  
Yellow Fever Virus ◽  
Vaccine Virus ◽  
Fever Virus ◽  
Productive Infection ◽  
Wild Type Virus ◽  
Type Virus ◽  
Wild Type

Yellow fever virus (YFV) causes serious disease in endemic areas of South America and Africa, even though a very well tolerated vaccine is available. YFV primarily targets the liver where as many as 80 % of hepatocytes may be involved during infection. The objective of this project was to compare and contrast the cytokine response from hepatocytes infected with either wild-type (Asibi) or vaccine (17-D-204) strains of YFV, with the goal of identifying responses that might be correlated with disease severity or vaccine efficacy. We report here that PH5CH8 hepatocytes support a productive infection with both wild-type and vaccine-strain YFV. Infection with either virus resulted in elevated expression of several pro- and anti-inflammatory cytokines [interleukin (IL)-1β, IL-4, IL-6, IL-8, IL-10 and tumour necrosis factor-α) with a corresponding increase in transcription. Hepatocytes infected with vaccine virus had a more profound response than did cells infected with wild-type virus. Pre-stimulation of hepatocytes with IL-6 resulted in reduced viral titres, elevated concentrations of cytokines released from Asibi virus-infected cells and improved cell viability in cells infected with 17-D virus. Data reported here suggest that 17-D virus stimulates an appropriate antiviral inflammatory response in hepatocytes, while Asibi virus can attenuate the host response. These data identify potential mechanisms that are associated with increased virulence in wild-type virus infections and also provide clues towards potential immune-response limitations that may be associated with vaccine-related adverse events.

scholarly journals Intranasal Interleukin-12 Treatment for Protection against Respiratory Infection with the Francisella tularensis Live Vaccine Strain

2005 ◽  
Vol 73 (4) ◽  
pp. 2306-2311 ◽  
Author(s):  
Nathalie S. Duckett ◽  
Sofia Olmos ◽  
Douglas M. Durrant ◽  
Dennis W. Metzger
Keyword(s):  
Respiratory Infection ◽  
Francisella Tularensis ◽  
Vaccine Strain ◽  
Live Vaccine ◽  
Interleukin 12 ◽  
Live Vaccine Strain ◽  
Wild Type ◽  
Intranasal Infection ◽  
Ifn Γ

ABSTRACT Francisella tularensis is a gram-negative intracellular bacterium that can induce lethal respiratory infection in humans and rodents. However, little is known about the role of innate or adaptive immunity in protection from respiratory tularemia. In the present study, the role of interleukin-12 (IL-12) in inducing protective immunity in the lungs against intranasal infection of mice with the live vaccine strain (LVS) of F. tularensis was investigated. It was found that gamma interferon (IFN-γ) and IL-12 were strictly required for protection, since mice deficient in IFN-γ, IL-12 p35, or IL-12 p40 all succumbed to LVS doses that were sublethal for wild-type mice. Furthermore, exogenous IL-12 treatment 24 h before intranasal infection with a lethal dose of LVS (10,000 CFU) significantly decreased bacterial loads in the lungs, livers, and spleens of wild-type BALB/c and C57BL/6 mice and allowed the animals to survive infection; such protection was not observed in IFN-γ-deficient mice. The resistance induced by IL-12 to LVS infection was still observed in NK cell-deficient beige mice but not in CD8−/− mice. These results demonstrate that exogenous IL-12 delivered intranasally can prevent respiratory tularemia through a mechanism that is at least partially dependent upon the expression of IFN-γ and CD8 T cells.

scholarly journals Molecular analysis of yellow fever virus 17DD vaccine strain

1991 ◽  
Vol 86 (2) ◽  
pp. 239-246 ◽  
Author(s):  
Paulo R. Post ◽  
Claudia N. D. Santos ◽  
Ricardo Carvalho ◽  
Oscar S. Lopes ◽  
Ricardo Galler
Keyword(s):  
Yellow Fever ◽  
Molecular Analysis ◽  
Vaccine Strain ◽  
Yellow Fever Virus ◽  
Fever Virus
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