Applications of Quasi Real-Time Metabolic Flux Analysis in Mammalian Cell Culture Process Development

Author(s):  
Chetan Goudar ◽  
Richard Biener ◽  
James Michaels ◽  
Chun ZHang ◽  
James Piret ◽  
...  
2012 ◽  
Vol 59 (1) ◽  
Author(s):  
Mohd Helmi Sani ◽  
Frank Baganz

At present, there are a number of commercial small scale shaken systems available on the market with instrumented controllable microbioreactors such as Micro–24 Microreactor System (Pall Corporation, Port Washington, NY) and M2P Biolector, (M2P Labs GmbH, Aachen, Germany). The Micro–24 system is basically an orbital shaken 24–well plate that operates at working volume 3 – 7 mL with 24 independent reactors (deep wells, shaken and sparged) running simultaneously. Each reactor is designed as single use reactor that has the ability to continuously monitor and control the pH, DO and temperature. The reactor aeration is supplied by sparging air from gas feeds that can be controlled individually. Furthermore, pH can be controlled by gas sparging using either dilute ammonia or carbon dioxide directly into the culture medium through a membrane at the bottom of each reactor. Chen et al., (2009) evaluated the Micro–24 system for the mammalian cell culture process development and found the Micro–24 system is suitable as scaledown tool for cell culture application. The result showed that intra-well reproducibility, cell growth, metabolites profiles and protein titres were scalable with 2 L bioreactors.


2010 ◽  
Vol 12 (2) ◽  
pp. 161-171 ◽  
Author(s):  
Lake-Ee Quek ◽  
Stefanie Dietmair ◽  
Jens O. Krömer ◽  
Lars K. Nielsen

2010 ◽  
Vol 105 (2) ◽  
pp. 260-275 ◽  
Author(s):  
Timothy A. Barrett ◽  
Andrew Wu ◽  
Hu Zhang ◽  
M. Susana Levy ◽  
Gary J. Lye

Sign in / Sign up

Export Citation Format

Share Document