Glycopeptide microarray for autoantibody detection in cancer

2011 ◽  
Vol 8 (4) ◽  
pp. 435-437 ◽  
Author(s):  
Celso A Reis ◽  
Diana Campos ◽  
Hugo Osório ◽  
Lúcio Lara Santos
Keyword(s):  
Autoantibody Detection

scholarly journals Expanding Assay Dynamics: A Combined Competitive and Direct Assay System for the Quantification of Proteins in Multiplexed Immunoassays

2008 ◽  
Vol 54 (6) ◽  
pp. 956-963 ◽  
Author(s):  
Michael Hartmann ◽  
Monika Schrenk ◽  
Anette Döttinger ◽  
Sarah Nagel ◽  
Johan Roeraade ◽  
...  
Keyword(s):  
Dynamic Range ◽  
Protein Microarray ◽  
Measurement Range ◽  
Multiplex Assay ◽  
Competitive System ◽  
Direct Immunoassay ◽  
Sandwich Configuration ◽  
Readout Channel ◽  
Autoantibody Detection ◽  
Detection And Quantification

Abstract Background: The concurrent detection and quantification of analytes that vary widely in concentration present a principal problem in multiplexed assay systems. Combining competitive and sandwich immunoassays permits coverage of a wide concentration range, and both highly abundant molecules and analytes present in low concentration can be quantified within the same assay. Methods: The use of different fluorescence readout channels allows the parallel use of a competitive system and a sandwich configuration. The 2 generated assay signals are combined and used to calculate the amount of analyte. The measurement range can be adjusted by varying the competitor concentration, and an extension of the assay system’s dynamic range is possible. Results: We implemented the method in a planar protein microarray–based autoimmune assay to detect autoantibodies against 13 autoantigens and to measure the concentration of a highly abundant protein, total human IgG, in one assay. Our results for autoantibody detection and IgG quantification agreed with results obtained with commercially available assays. The use of 2 readout channels in the protein microarray–based system reduced spot-to-spot variation and intraassay variation. Conclusions: By combining a direct immunoassay with a competitive system, analytes present in widely varying concentrations can be quantified within a single multiplex assay. Introducing a second readout channel for analyte quantification is an effective tool for spot-to-spot normalization and helps to lower intraassay variation.

scholarly journals Autoantibody Detection for Diagnosis in Direct Immunofluorescence-Negative Mucous Membrane Pemphigoid

Ophthalmology ◽  
2020 ◽  
Author(s):  
John Dart ◽  
Jane Setterfield ◽  
Richard W. Groves ◽  
John B. Mee ◽  
Gilles F.H. Diercks ◽  
...  
Keyword(s):  
Mucous Membrane ◽  
Mucous Membrane Pemphigoid ◽  
Direct Immunofluorescence ◽  
Autoantibody Detection

scholarly journals The autoantibody-mediated encephalitides: from clinical observations to molecular pathogenesis

2019 ◽  
Author(s):  
Sudarshini Ramanathan ◽  
Adam Al-Diwani ◽  
Patrick Waters ◽  
Sarosh R. Irani
Keyword(s):  
Cerebrospinal Fluid ◽  
Close Association ◽  
Autoimmune Encephalitis ◽  
Clinical Syndrome ◽  
Clinical Observations ◽  
Improved Outcomes ◽  
Clinical Syndromes ◽  
Specific Autoantibody ◽  
Autoantibody Detection

Abstract The autoimmune encephalitis (AE) syndromes have been characterised by the detection of autoantibodies in serum and/or cerebrospinal fluid which target the extracellular domains of specific neuroglial antigens. The clinical syndromes have phenotypes which are often highly characteristic of their associated antigen-specific autoantibody. For example, the constellation of psychiatric features and the multi-faceted movement disorder observed in patients with NMDAR antibodies are highly distinctive, as are the faciobrachial dystonic seizures observed in close association with LGI1 antibodies. These typically tight correlations may be conferred by the presence of autoantibodies which can directly access and modulate their antigens in vivo. AE remains an under-recognised clinical syndrome but one where early and accurate detection is critical as prompt initiation of immunotherapy is closely associated with improved outcomes. In this review of a rapidly emerging field, we outline molecular observations with translational value. We focus on contemporary methodologies of autoantibody detection, the evolution and distinctive nature of the clinical phenotypes, generalisable therapeutic paradigms, and finally discuss the likely mechanisms of autoimmunity in these patients which may inform future precision therapies.

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