Sequencing Control Region of Human Mitochondrial Genome to Assess Matrilineal Lineage

Author(s):  
Hirak Ranjan Dash ◽  
Pankaj Shrivastava ◽  
Surajit Das
2018 ◽  
Vol XIII (3) ◽  
Author(s):  
I.О. Маzunin

BMC Genomics ◽  
2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Wen-Ge Dong ◽  
Yalun Dong ◽  
Xian-Guo Guo ◽  
Renfu Shao

Abstract Background The typical single-chromosome mitochondrial (mt) genome of animals has fragmented into multiple minichromosomes in the lineage Mitodivisia, which contains most of the parasitic lice of eutherian mammals. These parasitic lice differ from each other even among congeneric species in mt karyotype, i.e. the number of minichromosomes, and the gene content and gene order in each minichromosome, which is in stark contrast to the extremely conserved single-chromosome mt genomes across most animal lineages. How fragmented mt genomes evolved is still poorly understood. We use Polyplax sucking lice as a model to investigate how tRNA gene translocation shapes the dynamic mt karyotypes. Results We sequenced the full mt genome of the Asian grey shrew louse, Polyplax reclinata. We then inferred the ancestral mt karyotype for Polyplax lice and compared it with the mt karyotypes of the three Polyplax species sequenced to date. We found that tRNA genes were entirely responsible for mt karyotype variation among these three species of Polyplax lice. Furthermore, tRNA gene translocation observed in Polyplax lice was only between different types of minichromosomes and towards the boundaries with the control region. A similar pattern of tRNA gene translocation can also been seen in other sucking lice with fragmented mt genomes. Conclusions We conclude that inter-minichromosomal tRNA gene translocation orientated towards the boundaries with the control region is a major contributing factor to the highly dynamic mitochondrial genome organization in the parasitic lice of mammals.


Gene ◽  
2015 ◽  
Vol 573 (2) ◽  
pp. 216-224 ◽  
Author(s):  
Xidong Mu ◽  
Xuejie Wang ◽  
Yi Liu ◽  
Hongmei Song ◽  
Chao Liu ◽  
...  

2011 ◽  
Vol 89 (8) ◽  
pp. 732-743 ◽  
Author(s):  
B.J. Wilson ◽  
S.J. Crockford ◽  
J.W. Johnson ◽  
R.S. Malhi ◽  
B.M. Kemp

Many well-preserved bones of medium-sized goose have been recovered from the Zeto Point archaeological site (ADK-011) on Adak Island in the central Aleutians, Alaska, that date to ca. 170–415 years before present based on conventional radiometric dates of the deposits. This prehistoric sample includes remains of adults and unfledged goslings that defied confident identification based on osteological criteria. While the presence of newborns indicates that Adak was a breeding ground, which species was doing the nesting remained uncertain. Of the five species of medium-sized goose (order Anseriformes, family Anatidae) known or presumed to visit Adak Island, three are rarely sighted. The only common visitor is the Emperor Goose ( Chen canagica (Sevastianov, 1802)). The Aleutian Cackling Goose ( Branta hutchinsii leucopareia (Brandt, 1836)) breeds elsewhere in the Aleutians but does not currently breed on Adak Island and there are no records of it nesting there in the past. Here DNA sequences from portions of the cytochrome b (cytb) gene and the control region (CR) of the mitochondrial genome were recovered from 28 of 29 Adak prehistoric goose remains. All adult specimens identified to species were either C. canagica or B. h. leuopareia, but all specifically identified juvenile specimens were B. h. leuopareia. The results demonstrate that Adak Island was a breeding ground of the Aleutian Cackling Goose prior to European contact.


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