Detection of Zika Virus Using Reverse Transcription-Loop-Mediated Isothermal Amplification (RT-LAMP)

2020 ◽  
pp. 137-146
Author(s):  
Laura E. Lamb ◽  
Sarah N. Bartolone ◽  
Michael B. Chancellor
Keyword(s):  
Reverse Transcription ◽  
Zika Virus ◽  
Isothermal Amplification ◽  
Loop Mediated Isothermal Amplification

scholarly journals Detection and discrimination of multiple strains of Zika virus by reverse transcription-loop-mediated isothermal amplification

2020 ◽  
Vol 48 (1) ◽  
Author(s):  
Hiroka Aonuma ◽  
Itoe Iizuka-Shiota ◽  
Tokio Hoshina ◽  
Shigeru Tajima ◽  
Fumihiro Kato ◽  
...  
Keyword(s):  
Virus Strain ◽  
Reverse Transcription ◽  
Zika Virus ◽  
Virus Disease ◽  
Isothermal Amplification ◽  
Lamp Method ◽  
Simple Method ◽  
Loop Mediated Isothermal Amplification ◽  
Specificity And Sensitivity ◽  
Virus Strains

Abstract Background Monitoring both invasion of Zika virus disease into free countries and circulation in endemic countries is essential to avoid a global pandemic. However, the difficulty lies in detecting Zika virus due to the large variety of mutations in its genomic sequence. To develop a rapid and simple method with high accuracy, reverse transcription-loop-mediated isothermal amplification (RT-LAMP) was adopted for the detection of Zika virus strains derived from several countries. Results Common primers for RT-LAMP were designed based on the genomic sequences of two standard Zika strains: African lineage, MR-766, and Asian lineage, PRVABC59. RT-LAMP reactions using a screened primer set, targeting the NS3 region, detected both Zika virus strains. The minimum detectable quantity was 3 × 10−2 ng of virus RNA. Measurable lag of reaction times among strains was observed. The RT-LAMP method amplified the target virus sequence from the urine and serum of a patient with a travel history in the Caribbean Islands and also provided a prediction about which lineage of Zika virus strain was present. Conclusions The RT-LAMP method using a well-optimized primer set demonstrated high specificity and sensitivity for the detection of Zika virus strains with a variety in genomic RNA sequences. In combination with the simplicity of LAMP reaction in isothermal conditions, the optimized primer set established in this study may facilitate rapid and accurate diagnosis of Zika fever patients with virus strain information.

scholarly journals Development and evaluation of a rapid molecular diagnostic test for Zika virus infection by reverse transcription loop-mediated isothermal amplification

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Yohei Kurosaki ◽  
Danyelly Bruneska Gondim Martins ◽  
Mayuko Kimura ◽  
Andriu dos Santos Catena ◽  
Maria Amélia Carlos Souto Maior Borba ◽  
...  
Keyword(s):  
Virus Infection ◽  
Diagnostic Test ◽  
Reverse Transcription ◽  
Zika Virus ◽  
Isothermal Amplification ◽  
Molecular Diagnostic ◽  
Loop Mediated Isothermal Amplification ◽  
Zika Virus Infection ◽  
Molecular Diagnostic Test

scholarly journals Rapid Detection of Zika Virus in Urine Samples and Infected Mosquitos by Reverse Transcription-Loop-Mediated Isothermal Amplification

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Laura E. Lamb ◽  
Sarah N. Bartolone ◽  
Maya O. Tree ◽  
Michael J. Conway ◽  
Julien Rossignol ◽  
...  
Keyword(s):  
Reverse Transcription ◽  
Zika Virus ◽  
Rapid Detection ◽  
Isothermal Amplification ◽  
Urine Samples ◽  
Loop Mediated Isothermal Amplification

scholarly journals Zika virus lateral flow assays using reverse transcription-loop-mediated isothermal amplification

RSC Advances ◽  
2021 ◽  
Vol 11 (29) ◽  
pp. 17800-17808
Author(s):  
Gna Ahn ◽  
SeonHyung Lee ◽  
Se Hee Lee ◽  
Yun Hee Baek ◽  
Min-Suk Song ◽  
...  
Keyword(s):  
Reverse Transcription ◽  
Zika Virus ◽  
Detection Method ◽  
Point Of Care ◽  
Lateral Flow ◽  
Isothermal Amplification ◽  
Independent Point ◽  
Loop Mediated Isothermal Amplification ◽  
Lateral Flow Assays ◽  
Point Of Care Detection

Our study suggest that ZIKV RT-LAMP combined with LFA could serve as a rapid, accurate, and independent point-of-care detection method for ZIKV outbreaks.

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