Contributions to Arabinogalactan Protein Analysis

Protein analysis of pediatric parapneumonic pleural effusion after intrapleural urokinase treatment

Cell Biology International ◽

10.1042/cbi034s069c ◽

2010 ◽

Vol 34 (8) ◽

pp. S69-S69

Author(s):

Jieh‑Neng Wang ◽

Pao‑Chi Liao ◽

Yu‑Chin Tasi ◽

Jing‑Ming Wu

Keyword(s):

Pleural Effusion ◽

Protein Analysis ◽

Parapneumonic Pleural Effusion

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Genetic diversity of durum wheat genotypes using morpho-protein analysis

Cereal Research Communications ◽

10.1556/crc.2014.0003 ◽

2014 ◽

Vol 42 (4) ◽

pp. 677-686

Author(s):

M. Rajabi Hashjin ◽

M.H. Fotokian ◽

M. Agahee Sarbrzeh ◽

M. Mohammadi ◽

D. Talei

Keyword(s):

Genetic Diversity ◽

Durum Wheat ◽

Protein Analysis ◽

Wheat Genotypes

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Characterisation of extracellular polysaccharides from suspension cultures of members of the Poaceae

10.26686/wgtn.12597854.v1 ◽

2020 ◽

Author(s):

Ian Sims ◽

K Middleton ◽

AG Lane ◽

AJ Cairns ◽

A Bacic

Keyword(s):

Cultured Cells ◽

Oryza Sativa L ◽

Suspension Cultures ◽

Exchange Chromatography ◽

Phleum Pratense ◽

Arabinogalactan Protein ◽

Extracellular Polysaccharides ◽

Type Ii ◽

Hordeum Vulgare L ◽

Suspension Cultured Cells

Microscopic examination of suspension-cultured cells of Phleum pratense L., Panicum miliaceum L., Phalaris aquatica L. and Oryza sativa L. showed that they were comprised of numerous root primordia. Polysaccharides secreted by these suspension cultures contained glycosyl linkages consistent with the presence of high proportions of root mucilage-like polysaccharides. In contrast, suspension-cultured cells of Hordeum vulgare L. contained mostly undifferentiated cells more typical of plant cells in suspension culture. The polysaccharides secreted by H. vulgare cultures contained mostly linkages consistent with the presence of glucuronoarabinoxylan. The soluble polymers secreted by cell-suspension cultures of Phleum pratense contained 70% carbohydrate, 14% protein and 6% inorganic material. The extracellular polysaccharides were separated into four fractions by anion-exchange chromatography using a gradient of imidazole-HCl at pH 7.0. From glycosyl-linkage analyses, five polysaccharides were identified: an arabinosylated xyloglucan (comprising 20% of the total polysaccharide), a glucomannan (6%), a type-II arabinogalactan (an arabinogalactan-protein; 7%), an acidic xylan (3%), and a root-slime-like polysaccharide, which contained features of type-II arabinogalactans and glucuronomannans (65%).

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Characterisation of extracellular polysaccharides from suspension cultures of members of the Poaceae

10.26686/wgtn.12597854 ◽

2020 ◽

Author(s):

Ian Sims ◽

K Middleton ◽

AG Lane ◽

AJ Cairns ◽

A Bacic

Keyword(s):

Cultured Cells ◽

Oryza Sativa L ◽

Suspension Cultures ◽

Exchange Chromatography ◽

Phleum Pratense ◽

Arabinogalactan Protein ◽

Extracellular Polysaccharides ◽

Type Ii ◽

Hordeum Vulgare L ◽

Suspension Cultured Cells

Microscopic examination of suspension-cultured cells of Phleum pratense L., Panicum miliaceum L., Phalaris aquatica L. and Oryza sativa L. showed that they were comprised of numerous root primordia. Polysaccharides secreted by these suspension cultures contained glycosyl linkages consistent with the presence of high proportions of root mucilage-like polysaccharides. In contrast, suspension-cultured cells of Hordeum vulgare L. contained mostly undifferentiated cells more typical of plant cells in suspension culture. The polysaccharides secreted by H. vulgare cultures contained mostly linkages consistent with the presence of glucuronoarabinoxylan. The soluble polymers secreted by cell-suspension cultures of Phleum pratense contained 70% carbohydrate, 14% protein and 6% inorganic material. The extracellular polysaccharides were separated into four fractions by anion-exchange chromatography using a gradient of imidazole-HCl at pH 7.0. From glycosyl-linkage analyses, five polysaccharides were identified: an arabinosylated xyloglucan (comprising 20% of the total polysaccharide), a glucomannan (6%), a type-II arabinogalactan (an arabinogalactan-protein; 7%), an acidic xylan (3%), and a root-slime-like polysaccharide, which contained features of type-II arabinogalactans and glucuronomannans (65%).

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Structure of the exudate gum from Meryta sinclairii

10.26686/wgtn.12597851.v1 ◽

2020 ◽

Author(s):

Ian Sims ◽

Richard Furneaux

Keyword(s):

Average Molecular Weight ◽

Gum Arabic ◽

Arabinogalactan Protein ◽

Weight Average Molecular Weight ◽

1H And 13C Nmr ◽

One Dimensional ◽

Linkage Analyses ◽

Yariv Reagent ◽

Native Tree ◽

High Level

A gum that exudes from the wounded trunk of the New Zealand native tree Meryta sinclairii has been isolated. The gum was completely precipitated by the β-glucosyl Yariv reagent and was thus determined to be an arabinogalactan-protein (AGP). It contained >95% w/w carbohydrate and only 2% w/w protein with a high level of hydroxyproline. SEC-MALLS showed that the gum had a weight-average molecular weight of 4.45×106Da compared with 6.02×105Da for gum arabic. Constituent sugar and linkage analyses were consistent with polymers comprised of a highly branched backbone of 1,3-linked galactopyranosyl (Galp) residues, with side-chains made up of arabinofuranose- (Araf) containing oligosaccharides, terminated variously by rhamnopyranosyl (Rhap), arabinopyranosyl (Arap), Galp and glucuronopyranosyl (GlcpA) residues. Analysis by one-dimensional and two-dimensional 1H and 13C NMR experiments confirmed the linkage analyses. The structure of the gum is discussed in comparison with the structure of gum arabic and other AGPs. © 2003 Elsevier Science Ltd. All rights reserved.

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Molecular, rheological and physicochemical characterisation of puka gum, an arabinogalactan-protein extracted from the Meryta sinclairii tree

10.26686/wgtn.12585629.v1 ◽

2020 ◽

Author(s):

MSM Wee ◽

Ian Sims ◽

KKT Goh ◽

L Matia-Merino

Keyword(s):

Hydrodynamic Radius ◽

Average Molecular Weight ◽

Gum Arabic ◽

Water Soluble ◽

Arabinogalactan Protein ◽

Type Ii ◽

Weight Average Molecular Weight ◽

Soluble Polysaccharide ◽

Physicochemical Characterisation ◽

Increasing Temperature

© 2019 Elsevier Ltd A water-soluble polysaccharide (type II arabinogalactan-protein) extracted from the gum exudate of the native New Zealand puka tree (Meryta sinclairii), was characterised for its molecular, rheological and physicochemical properties. In 0.1 M NaCl, the weight average molecular weight (Mw) of puka gum is 5.9 × 106 Da with an RMS radius of 56 nm and z-average hydrodynamic radius of 79 nm. The intrinsic viscosity of the polysaccharide is 57 ml/g with a coil overlap concentration 15% w/w. Together, the shape factor, p, of 0.70 (exponent of RMS radius vs. hydrodynamic radius), Smidsrød-Haug's stiffness parameter B of 0.031 and Mark-Houwink exponent α of 0.375 indicate that the polysaccharide adopts a spherical conformation in solution, similar to gum arabic. The pKa is 1.8. The polysaccharide exhibits a Newtonian to shear-thinning behaviour from 0.2 to 25% w/w. Viscosity of the polysaccharide (1 s−1) decreases with decreasing concentration, increasing temperature, ionic strength, and at acidic pH.

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Extracellular polysaccharides from suspension cultures of Nicotiana plumbaginifolia

10.26686/wgtn.12597827.v1 ◽

2020 ◽

Author(s):

Ian Sims ◽

A Bacic

Keyword(s):

Uronic Acid ◽

Cell Suspension Cultures ◽

Anion Exchange Chromatography ◽

Nicotiana Plumbaginifolia ◽

Suspension Cultures ◽

Exchange Chromatography ◽

Arabinogalactan Protein ◽

Extracellular Polysaccharides ◽

Selective Precipitation ◽

The Individual

The soluble polymers secreted by cell-suspension cultures of Nicotiana plumbaginifolia contained 78% carbohydrate, 6% protein and 4% inorganic material. The extracellular polysaccharides were separated into three fractions by anion-exchange chromatography using a gradient of imidazole-HCl at pH 7 and the individual polysaccharides in each fraction were then isolated by selective precipitation and enzymic treatment. Monosaccharide and linkage compositions were determined for each polysaccharide after reduction of uronic acid residues and the degree of esterification of the various uronic acid residues in each polysaccharide was determined concurrently with the linkage types. Six components were identified: an arabinoxyloglucan (comprising 34% of the total polysaccharide) and a galactoglucomannan (15%) in the unbound neutral fraction, a type II arabinogalactan (an arabinogalactan-protein, 11%) and an acidic xylan (3%) in the first bound fraction, and an arabinoglucuronomannan (11%) and a galacturonan (26%) in the second bound fraction. © 1995.

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