Genetic diversity of durum wheat genotypes using morpho-protein analysis

Abstract Analysis of genetic diversity provides helpful information necessary to develop the breeding and conservation strategies of crops. In this study, the genetic diversity and population structure of 90 durum wheat genotypes maintained at Sararud Dryland Agricultural Research Institute, Kermanshah, Iran, were evaluated by using 23 gene-specific markers (functional markers, FMs) encoding high and low molecular weight glutenin and gliadin alleles. Results showed that 12 out of the 23 FMs used were polymorphic and amplified 52 polymorphic loci. Primer Ax2 ⃰ had the highest discriminatory power. The population structure analysis classified the durum wheat collection into four populations. On average, population 4, consisting of 8 genotypes, had the highest allele number as well as genetic variation. Analysis of molecular variance indicated that 82% of the total variation was distributed among populations. The diversity among populations and gene flow were 0.14 and 3.03, respectively. The Jaccard distance coefficient revealed that genetic dissimilarities ranged from 0.031 between G62 and G65 to 0.725 between G36 and G51. Neighbor-joining method clustered individuals into six main groups. Results showed a remarkable level of genetic diversity among studied durum wheat genotypes which can be of interest for future breeding programs.

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The study of genetic diversity in a minicore collection of durum wheat genotypes using agro-morphological traits and molecular markers

Cereal Research Communications ◽

10.1007/s42976-020-00073-6 ◽

2020 ◽

Author(s):

N. Shaygan ◽

A. Etminan ◽

I. Majidi Hervan ◽

R. Azizinezhad ◽

R. Mohammadi

Keyword(s):

Genetic Diversity ◽

Molecular Markers ◽

Durum Wheat ◽

Morphological Traits ◽

Wheat Genotypes

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Evaluation of genetic diversity of durum wheat (Triticum durum desf.) genotypes using inter-simple sequence repeat (ISSR) and caat box-derived polymorphism (CBDP) markers

Genetika ◽

10.2298/gensr2003895a ◽

2020 ◽

Vol 52 (3) ◽

pp. 895-909

Author(s):

Mahmood Aslan-Parviz ◽

Mansoor Omidi ◽

Varahram Rashidi ◽

Alireza Etminan ◽

Alireza Ahmadzadeh

Keyword(s):

Genetic Diversity ◽

Durum Wheat ◽

Simple Sequence Repeat ◽

Genetic Parameters ◽

Inter Simple Sequence Repeat ◽

Molecular Technique ◽

Sequence Repeat ◽

Wheat Genotypes ◽

Simple Sequence ◽

Issr Primers

Evaluation of genetic diversity is the key principal for plant breeding, providing an opportunity to discover novel characters and alleles for breeders. In the present study, 69 durum wheat genotypes were investigated for genetic diversity using several CAAT box-derived polymorphism (CBDP) and inter-simple sequence repeat (ISSR) markers. Twelve CBDP and sixteen ISSR primers amplified a total of 115 and 160 polymorphic fragments with a mean of 9.58 and 10 fragments per primer, respectively. CBDP primers showed the higher mean values for informativeness parameters such as polymorphic information content (PIC), resolving power (Rp) and marker index (MI) in comparison with ISSR primers. The results of analysis of molecular variance (AMOVA) indicated that the highest proportion of genetic variance referred within populations. Furthermore, CBDP primers indicated high values for all genetic parameters. Besides, the highest values of genetic parameters including number of observed (Na) and effective alleles (Ne), Shannon?s information index (I) and Nei?s gene diversity (He) were estimated for Iranian durum wheat landraces. Cluster analysis based on each molecular technique classified all durum wheat genotypes into three main groups, so that the results of principal coordinate analysis (PCoA) supported the grouping patterns. As a result, the grouping pattern observed by ISSR primers was clearer than CBDP primers and grouped all samples based on their origins. However, Mantel?s coefficient correlation test illustrated the higher positive correlation (0.54) between both marker techniques. Hence, the use of these markers in combination with each other to evaluate the genetic diversity is recommended.

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Genetic diversity of high and low molecular weight glutenin subunits in Saharan bread and durum wheats from Algerian oases

Czech Journal of Genetics and Plant Breeding ◽

10.17221/105/2011-cjgpb ◽

2012 ◽

Vol 48 (No. 1) ◽

pp. 23-32 ◽

Cited By ~ 11

Author(s):

I. Bellil ◽

M. Chekara Bouziani ◽

D. Khelifi

Keyword(s):

Genetic Diversity ◽

Molecular Weight ◽

Durum Wheat ◽

Bread Wheat ◽

Allelic Variation ◽

Low Molecular Weight ◽

Glutenin Subunits ◽

Lmw Glutenin Subunits ◽

Genotypic Identification ◽

Diversity Studies

Saharan wheats have been studied particularly from a botanical viewpoint. Genotypic identification, classification and genetic diversity studies to date were essentially based on the morphology of the spike and grain. For this, the allelic variation at the glutenin loci was studied in a set of Saharan bread and durum wheats from Algerian oases where this crop has been traditionally cultivated. The high molecular weight and low molecular weight glutenin subunit composition of 40 Saharan bread and 30 durum wheats was determined by SDS-PAGE. In Saharan bread wheats 32 alleles at the six glutenin loci were detected, which in combination resulted in 36 different patterns including 17 for HMW and 23 for LMW glutenin subunits. For the Saharan durum wheats, 29 different alleles were identified for the five glutenin loci studied. Altogether, 29 glutenin patterns were detected, including 13 for HMW-GS and 20 for LMW-GS. Three new alleles were found in Saharan wheats, two in durum wheat at the Glu-B1 and Glu-B3 loci, and one in bread wheat at the Glu-B1 locus. The mean indices of genetic variation at the six loci in bread wheat and at the five loci in durum wheat were 0.59 and 0.63, respectively, showing that Saharan wheats were more diverse. This information could be useful to select Saharan varieties with improved quality and also as a source of genes to develop new lines when breeding for quality.

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