Arabidopsis thaliana Mature Endosperm Dissection and Isolation of Genomic DNA from Mature Seed Tissues

2021 ◽  
pp. 239-243
Author(s):  
Mayumi Iwasaki ◽  
Luis Lopez-Molina
Keyword(s):  
Arabidopsis Thaliana ◽  
Genomic Dna ◽  
Mature Seed ◽  
Mature Endosperm

scholarly journals Capture and Purification of Plant Genomic DNA on a Readily-available Cellulose Matrix

2017 ◽  
Author(s):  
Megan M. Thompson ◽  
Estelle M. Hrabak
Keyword(s):  
Arabidopsis Thaliana ◽  
Nucleic Acids ◽  
Plant Species ◽  
Efficient Method ◽  
Genomic Dna ◽  
Low Cost ◽  
Long Term Storage ◽  
Cellulose Matrix ◽  
Term Storage

AbstractWhatman FTA ®Cards are a fast and efficient method for capturing and storing nucleic acids but are cost-prohibitive for some researchers. We developed a method that substitutes readily-available cellulose-based paper and homemade washing buffer for commercial FTA ®Cards and FTA ®Purification Reagent. This method is suitable for long-term storage of DNA from many plant species, including Arabidopsis thaliana, prior to purification and PCR.Method SummaryHere we report a low-cost method for long-term storage of plant genomic DNA on a readily available cellulose matrix.

Identification and analysis of homoeologous segments of the genomes of rice and Arabidopsis thaliana

Genome ◽  
1999 ◽  
Vol 42 (5) ◽  
pp. 887-892 ◽  
Author(s):  
Anne-Marie van Dodeweerd ◽  
Caroline R Hall ◽  
Elisabeth G Bent ◽  
Samantha J Johnson ◽  
Michael W Bevan ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Dna Sequences ◽  
Genomic Dna ◽  
Genome Structure ◽  
Plant Genome ◽  
Fine Scale ◽  
Plant Genomics ◽  
Homologous Genes ◽  
Conserved Genes ◽  
Plant Genome Evolution

Using contiguous genomic DNA sequences of Arabidopsis thaliana, we were able to identify a region of conserved structure in the genome of rice. The conserved, and presumptive homoeologous segments, are 194 kb and 219-300 kb in size in Arabidopsis and rice, respectively. They contain five homologous genes, distinguished in order by a single inversion. These represent the first homoeologous segments identified in the genomes of a dicot and a monocot, demonstrating that fine-scale conservation of genome structure exists and is detectable across this major divide in the angiosperms. The conserved framework of genes identified is interspersed with non-conserved genes, indicating that mechanisms beyond segmental inversions and translocations need to be invoked to fully explain plant genome evolution, and that the benefits of comparative genomics over such large taxonomic distances may be limited.Key words: plant genomics, comparative mapping.

Genomic DNA structure of a gene encoding cytosolic ascorbate peroxidase from Arabidopsis thaliana

FEBS Letters ◽  
1993 ◽  
Vol 315 (3) ◽  
pp. 313-317 ◽  
Author(s):  
Akihiro Kubo ◽  
Hikaru Saji ◽  
Kiyoshi Tanaka ◽  
Noriaki Kondo
Keyword(s):  
Arabidopsis Thaliana ◽  
Ascorbate Peroxidase ◽  
Genomic Dna ◽  
Dna Structure ◽  
Gene Encoding ◽  
Cytosolic Ascorbate Peroxidase

Stable genetic transformation of Arabidopsis thaliana by Agrobacterium inoculation in planta

1994 ◽  
Vol 5 (4) ◽  
pp. 551-558 ◽  
Author(s):  
Seok So Chang ◽  
Soon Ki Park ◽  
Byung Chul Kim ◽  
Bong Joong Kang ◽  
Dal Ung Kim ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Genetic Transformation ◽  
In Planta
Sign in / Sign up

Export Citation Format

Share Document