Express Arabidopsis Cryptochrome in Sf9 Insect Cells Using the

2021 ◽  
pp. 155-160
Author(s):  
Xu Li ◽  
Yawen Liu ◽  
Hongtao Liu
Keyword(s):  
Insect Cells ◽  
Sf9 Insect Cells

scholarly journals Expression and Immunogenicity of Two Recombinant Fusion Proteins Comprising Foot-and-Mouth Disease Virus Structural Protein VP1 and DC-SIGN-Binding Glycoproteins

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Xinsheng Liu ◽  
Jianliang Lv ◽  
Yuzhen Fang ◽  
Peng Zhou ◽  
Yanzhen Lu ◽  
...  
Keyword(s):  
Immune Responses ◽  
Disease Virus ◽  
Fusion Proteins ◽  
Insect Cells ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Vp1 Protein ◽  
Sf9 Insect Cells ◽  
Mouth Disease Virus ◽  
Foot And Mouth

Improving vaccine immunogenicity by targeting antigens to dendritic cells has recently emerged as a new design strategy in vaccine development. In this study, the VP1 gene of foot-and-mouth disease virus (FMDV) serotype A was fused with the gene encoding human immunodeficiency virus (HIV) membrane glycoprotein gp120 or C2-V3 domain of hepatitis C virus (HCV) envelope glycoprotein E2, both of which are DC-SIGN-binding glycoproteins. After codon optimization, the VP1 protein and the two recombinant VP1-gp120 and VP1-E2 fusion proteins were expressed in Sf9 insect cells using the insect cell-baculovirus expression system. Western blotting showed that the VP1 protein and two recombinant VP1-gp120 and VP1-E2 fusion proteins were correctly expressed in the Sf9 insect cells and had good reactogenicity. Guinea pigs were then immunized with the purified proteins, and the resulting humoral and cellular immune responses were analyzed. The VP1-gp120 and VP1-E2 fusion proteins induced significantly higher specific anti-FMDV antibody levels than the VP1 protein and stronger cell-mediated immune responses. This study provides a new perspective for the development of novel FMDV subunit vaccines.

scholarly journals Sesquiterpene Lactones from Artemisia absinthium. Biotransformation and Rearrangement of the Insect Antifeedant 3α-hydroxypelenolide

Plants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 891
Author(s):  
Braulio M. Fraga ◽  
Carmen E. Díaz ◽  
María Bailén ◽  
Azucena González-Coloma
Keyword(s):  
Cytotoxic Activity ◽  
Leptinotarsa Decemlineata ◽  
Spodoptera Littoralis ◽  
Culture Medium ◽  
Insect Cells ◽  
Sesquiterpene Lactones ◽  
Enzymatic System ◽  
Artemisia Absinthium ◽  
Sf9 Insect Cells ◽  
New Compounds

Three new compounds, the sesquiterpenes absilactone and hansonlactone and the acetophenone derivative ajenjol, have been isolated from a cultivated variety of Artemisia absinthium. In addition, the major lactone isolated, 3α-hydroxypelenolide, was biotransformed by the fungus Mucor plumbeus affording the corresponding 1β, 10α-epoxide. A cadinane derivative was formed by an acid rearrangement produced in the culture medium, but not by the enzymatic system of the fungus. Furthermore, 3α-hydroxypelenolide showed strong antifeedant effects against Leptinotarsa decemlineata and cytotoxic activity to Sf9 insect cells, while the biotransformed compounds showed antifeedant postingestive effects against Spodoptera littoralis.

Purification of Recombinant p53 from Sf9 Insect Cells

p53 Protocols ◽  
2003 ◽  
pp. 17-28
Author(s):  
Xiu Zhu Sun ◽  
John Nguyen ◽  
Jamil Momand
Keyword(s):  
Insect Cells ◽  
Sf9 Insect Cells

Human DRA Functions as a Sulfate Transporter in Sf9 Insect Cells

1998 ◽  
Vol 12 (1) ◽  
pp. 67-74 ◽  
Author(s):  
Mee Kyeong Byeon ◽  
Arthur Frankel ◽  
Takis S. Papas ◽  
Kelly W. Henderson ◽  
Clifford W. Schweinfest
Keyword(s):  
Insect Cells ◽  
Sulfate Transporter ◽  
Sf9 Insect Cells

scholarly journals Cold-active extracellular lipase: Expression in Sf9 insect cells, purification, and catalysis

2019 ◽  
Vol 21 ◽  
pp. e00295 ◽  
Author(s):  
Tang Li ◽  
Wenfa Zhang ◽  
Jianhua Hao ◽  
Mi Sun ◽  
Sheng-Xiang Lin
Keyword(s):  
Insect Cells ◽  
Extracellular Lipase ◽  
Sf9 Insect Cells ◽  
Cold Active ◽  
Lipase Expression

Human histidine-rich glycoprotein expressed in SF9 insect cells inhibits apatite formation

FEBS Letters ◽  
1997 ◽  
Vol 412 (3) ◽  
pp. 559-562 ◽  
Author(s):  
Thorsten Schinke ◽  
Takehiko Koide ◽  
Willi Jahnen-Dechent
Keyword(s):  
Insect Cells ◽  
Apatite Formation ◽  
Sf9 Insect Cells

Pharmacological and structural integrity of muscarinic M2 acetylcholine receptors produced in Sf9 insect cells

1997 ◽  
Vol 333 (2-3) ◽  
pp. 269-278 ◽  
Author(s):  
Claire Weill ◽  
François Autelitano ◽  
Chantal Guenet ◽  
Freddy Heitz ◽  
Maurice Goeldner ◽  
...  
Keyword(s):  
Acetylcholine Receptors ◽  
Structural Integrity ◽  
Insect Cells ◽  
Sf9 Insect Cells
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