Histochemical Analysis of Heparan 3-O-sulfotransferase Expression in Mouse

The histopathological effects of fumonisin B1 (FB1) injected intraperitoneally (IP), was evaluated in catfish (Ictalurus punctatus). Fishes were divided into four Groups. Groups II, III and IV were treated IP with FB1 injections of 1; 5 and 10 mg/kg bw/day, respectively, during 21 days. At the 7th, 14th and 21st day, fishes were sacrificed. The livers were hystologicaly analysed by the light and transmission electronic microscopy. Livers from the 7th day showed organelles alterations, particularly in the granular endoplasmatic reticle, mitochondria, nucleus and nucleolus mediated by FB1 doses. The occurrence of processes involved in the necrosis and apoptosis was detected. At the highest FB1 dose,the livers presented an intense response with an accentuate tissue disorganization, absence of cell limits and intense cytoplasm vacuolization. The image analysis showed the occurrence of necrosis in some areas, characterized by fully broken or swollen cells. The apoptosis was observed as the cytoplasm contraction and the chromatin formed masses concentrated in the edge of the nucleus. There was strong evidence that the numerous hepatocytes in the liver from the fishes under the toxic dose of FBs were selectively removed by the apoptosis process.

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A histochemical analysis of vascular and nonvascular smooth muscles in the canine lung

Microvascular Research ◽

10.1016/0026-2862(79)90026-8 ◽

1979 ◽

Vol 18 (2) ◽

pp. 167-174 ◽

Cited By ~ 2

Author(s):

R.E. Parker ◽

D.N. Granger ◽

B.H. Cook ◽

H.J. Granger ◽

A.E. Taylor

Keyword(s):

Smooth Muscles ◽

Histochemical Analysis

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Lymphangiomyomatosis—immuno-Histochemical Analysis of a Case Presenting with Enlarged Inguinal Lymph Nodes and Without Pulmonary Involvement

Acta Oncologica ◽

10.3109/02841868909111265 ◽

1989 ◽

Vol 28 (2) ◽

pp. 287-289

Author(s):

A. Österborg ◽

B. Christensson ◽

C. Silfverswärd ◽

P. Biberfeld ◽

V. P. Collins ◽

...

Keyword(s):

Lymph Nodes ◽

Pulmonary Involvement ◽

Histochemical Analysis ◽

Inguinal Lymph Nodes

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Colocalization of ghrelin O-acyltransferase and ghrelin in gastric mucosal cells

AJP Endocrinology and Metabolism ◽

10.1152/ajpendo.90859.2008 ◽

2009 ◽

Vol 297 (1) ◽

pp. E134-E141 ◽

Cited By ~ 83

Author(s):

Ichiro Sakata ◽

Jing Yang ◽

Charlotte E. Lee ◽

Sherri Osborne-Lawrence ◽

Sherry A. Rovinsky ◽

...

Keyword(s):

Peptide Hormone ◽

Whole Body ◽

Histochemical Analysis ◽

Oxyntic Mucosa ◽

Body Distribution ◽

Naturally Occurring ◽

Mucosal Cells ◽

Membrane Bound ◽

High Degree ◽

Dual Label

Ghrelin is a peptide hormone with many known functions, including orexigenic, blood glucose-regulatory, and antidepressant actions, among others. Mature ghrelin is unique in that it is the only known naturally occurring peptide to be posttranslationally modified by O-acylation with octanoate. This acylation is required for many of ghrelin's actions, including its effects on promoting increases in food intake and body weight. GOAT (ghrelin O-acyltransferase), one of 16 members of the MBOAT family of membrane-bound O-acyltransferases, has recently been identified as the enzyme responsible for catalyzing the addition of the octanoyl group to ghrelin. Although the initial reports of GOAT have localized its encoding mRNA to tissues known to contain ghrelin, it is as yet unclear whether the octanoylation occurs within ghrelin-producing cells or in neighboring cells. Here, we have performed dual-label histochemical analysis on mouse stomach sections and quantitative PCR on mRNAs from highly enriched pools of mouse gastric ghrelin cells to demonstrate a high degree of GOAT mRNA expression within ghrelin-producing cells of the gastric oxyntic mucosa. We also demonstrate that GOAT is the only member of the MBOAT family whose expression is highly enriched within gastric ghrelin cells and whose whole body distribution mirrors that of ghrelin.

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Histochemical analysis of skeletal muscular tissues of pigs according to genotype MYF4

Biotechnology in Animal Husbandry ◽

10.2298/bah0802117z ◽

2008 ◽

Vol 24 (1-2) ◽

pp. 117-126

Author(s):

Vladimir Zimmermann ◽

V. Kulísek ◽

A. Copík ◽

M. Odstrcil ◽

Ondrej Debrecéni ◽

...

Keyword(s):

Rectus Femoris ◽

Muscle Fibres ◽

Fat Cells ◽

Average Thickness ◽

Triceps Brachii ◽

Surface Representation ◽

Histochemical Analysis ◽

Pcr Method ◽

Muscular Tissues

The results of histochemical analysis of three muscles m. triceps brachii (MTB), m. longissimus thoracicus (MLT) and m. rectus femoris (MRF) of two groups of pigs created according to the genotypes MYF 4 are presented. Determination of MYF 4 genotypes was made by PCR method and for histochemical analysis was used 5 animals detected as homozygote MYF 4- AA type and 5 animals of heterozygote genotype myogenin-AB out of the total of 25 individual animals tested. The histochemical analysis proved that homozygotes AA have had bigger fat cells than heterozygotes AB in three studied muscles in average. The size of fat cells in MLT - 41.10?m or 38.50 ?m respectively dominated in both groups of animals. Percentage surface representation of interstitial tissues was higher in the studied muscles of heterozygote MYF 4-AB. The volume of ligaments was the highest in MRF (3.80% or 3.90% respectively) in both groups (myogenin - AA and AB). The average thickness was of three studied muscles muscle fibres higher at homozygote genotype myogenin-AA than in heterozygote myogenin-AB. The thickest fibres in both genotypes were in MRF (88.60 ?m, and 84.72 ?m respectively) and the lowest in MTB (73.30 and 69.40 ?m respectively). The highest values of muscle fibres thickness were detected in ?-White fibres. Their percentage surface representation corresponded to this in all three types of muscles of both studied genotype myogenin groups.

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