scholarly journals Histochemical analysis of skeletal muscular tissues of pigs according to genotype MYF4

2008 ◽  
Vol 24 (1-2) ◽  
pp. 117-126
Author(s):  
Vladimir Zimmermann ◽  
V. Kulísek ◽  
A. Copík ◽  
M. Odstrcil ◽  
Ondrej Debrecéni ◽  
...  
Keyword(s):  
Rectus Femoris ◽  
Muscle Fibres ◽  
Fat Cells ◽  
Average Thickness ◽  
Triceps Brachii ◽  
Surface Representation ◽  
Histochemical Analysis ◽  
Pcr Method ◽  
Muscular Tissues

The results of histochemical analysis of three muscles m. triceps brachii (MTB), m. longissimus thoracicus (MLT) and m. rectus femoris (MRF) of two groups of pigs created according to the genotypes MYF 4 are presented. Determination of MYF 4 genotypes was made by PCR method and for histochemical analysis was used 5 animals detected as homozygote MYF 4- AA type and 5 animals of heterozygote genotype myogenin-AB out of the total of 25 individual animals tested. The histochemical analysis proved that homozygotes AA have had bigger fat cells than heterozygotes AB in three studied muscles in average. The size of fat cells in MLT - 41.10?m or 38.50 ?m respectively dominated in both groups of animals. Percentage surface representation of interstitial tissues was higher in the studied muscles of heterozygote MYF 4-AB. The volume of ligaments was the highest in MRF (3.80% or 3.90% respectively) in both groups (myogenin - AA and AB). The average thickness was of three studied muscles muscle fibres higher at homozygote genotype myogenin-AA than in heterozygote myogenin-AB. The thickest fibres in both genotypes were in MRF (88.60 ?m, and 84.72 ?m respectively) and the lowest in MTB (73.30 and 69.40 ?m respectively). The highest values of muscle fibres thickness were detected in ?-White fibres. Their percentage surface representation corresponded to this in all three types of muscles of both studied genotype myogenin groups.

scholarly journals Histochemical analysis of skeletal muscular tissues of pigs according to genotype <i>MYF 4</i> (Short communication)

2009 ◽  
Vol 52 (4) ◽  
pp. 395-401
Author(s):  
P. Makovický ◽  
P. Makovický ◽  
M. Kačániová ◽  
S. Pavličová ◽  
P. Haščík
Keyword(s):  
Short Communication ◽  
Rectus Femoris ◽  
Polymerase Chain Reaction Method ◽  
Muscle Fibres ◽  
Triceps Brachii ◽  
Chain Reaction ◽  
Histochemical Analysis ◽  
Polymerase Chain ◽  
Muscular Tissues

Abstract. The results of histochemical analysis of 3 muscles – m. triceps brachii (MTB), m. longissimus thoracitus (MLT) and m. rectus femoris (MRF) – in 2 groups of pigs formed according to the genotypes MYF 4 are presented in this study. Determination of MYF 4 genotypes was performed by the polymerase chain reaction method (PCR). For histochemical analysis, 5 animals detected as homozygotes of the MYF 4-AA type and 5 animals of the heterozygous genotype AB were used out of the total 25 individual animals tested. The histochemical analysis demonstrated that homozygotes AA had larger fat cells on the average than heterozygotes AB in the three studied muscles, which was statistically significant (P<0.05). The percentage surface representation of interstitial tissues was higher in the studied muscles of heterozygotes MYF 4-AB. The volume of interstitial tissues was the highest in the MRF in both groups (myogenin – AA and AB). The average thickness of muscle fibres of the three studied muscles was higher in the homozygote genotype myogenin MYF 4-AA than in heterozygotes MYF 4-AB, which was statistically significant (P<0.05).

scholarly journals Identification of the Trichinella species by PCR method

2012 ◽  
Vol 66 (1-2) ◽  
pp. 41-47
Author(s):  
Sasa Vasilev ◽  
Jelena Cvetkovic ◽  
Ivana Radovic ◽  
Ljiljana Sofronic-Milosavljevic
Keyword(s):  
Human Health ◽  
Health Problem ◽  
Continuous Monitoring ◽  
Human Consumption ◽  
Sylvatic Cycle ◽  
Trichinella Infection ◽  
High Prevalence ◽  
Pcr Method ◽  
Pig Meat

Serbia is country with a high prevalence of Trichinella infection in pigs, which continues to be a serious human health problem. In Serbia, only a few isolates of Trichinella found in pork have been genetically specified to date, and all were proven as T. spiralis. New data shows that in the sylvatic cycle in Serbia, at least in the Belgrade district, more than one Trichinella species co-exist (T. spiralis and T. britovi). Increased awareness of the possible overlap among sylvatic and domestic Trichinella cycles indicates the need for continuous monitoring of Trichinella species circulation and strongly points to the need that all isolates of Trichinella found in meat for human consumption should be subject to a determination of the Trichinella species (due to the risk of transmission of infection with T. britovi to domestic pigs and humans). This is why we examined using PCR the Trichinella larvae found in pig meat that caused a human outbreak (Trichinella infection) in Grocka (Belgrade district) during February 2011. The isolated larvae belonged to T. spiralis.

Determination of Specific Class E Immunoglobulins to Bet v 1 Birch Allergen by the Immuno-PCR Method

2018 ◽  
Vol 44 (2) ◽  
pp. 217-224 ◽  
Author(s):  
M. A. Simonova ◽  
V. D. Pivovarov ◽  
D. Yu. Ryazantsev ◽  
M. A. Kostromina ◽  
T. I. Muravieva ◽  
...  
Keyword(s):  
Specific Class ◽  
Bet V 1 ◽  
Pcr Method ◽  
Class E

Gross Morphological and Sex Wise Morphometrical Studies on Mandible of Adult Blue Bull (Boselaphus tragocamelus)

2021 ◽  
Author(s):  
S. Sathapathy ◽  
B.S. Dhote ◽  
S.K. Bharti ◽  
I. Singh
Keyword(s):  
Statistical Analysis ◽  
Average Length ◽  
T Test ◽  
Average Thickness ◽  
Legal Cases ◽  
Recorded Data ◽  
Mandibular Notch ◽  
Horizontal Ramus ◽  
Wild Life

Background: The Blue bull (Boselaphus tragocamelus) is one of the biggest antelopes in Asia and is widely distributed in both the forests and adjoining villages with enough green grass.Methods: The present study was carried out on the mandible of six specimens of adult Blue bull (Boselaphus tragocamelus) of either sex. The biometrical parameters were measured by scale, graduated tape and digital Vernier’s caliper. The statistical analysis of the recorded data was done by independent samples t-Test with Systat Software Inc, USA and SPSS 16.0 version software.Result: The mandible of Blue bull consisted of two rami, i.e. horizontal and vertical rami. The two halves of this bone fused incompletely at the mandibular symphysis, situated at the midline. The average length of horizontal ramus of mandible was found to be 24.7±1.02 cm in female, which was significantly less (P less than 0.05) than that of males, where it was recorded as 35.4±1.97 cm. Similarly, the average thickness of vertical ramus at the base was found to be 0.53±0.001 cm in female, which was significantly less (P less than 0.05) than that of males, where it was recorded as 0.80±0.002 cm. The average cranio-caudal length of mandibular notch was found to be 1.32±0.01 cm in female, which was significantly less (P less than 0.05) than that of males, where it was recorded as 1.44±0.02 cm. Most of the biometrical observations on different parameters of mandible of Blue bull were having significantly (P less than 0.05) more values in males than females. Conclusion: Most of the biometrical observations on different parameters of mandible of Blue bull were having significantly (p less than 0.05) more values in males than females. The present gross and biometrical studies would be useful to the wild life professionals for determination of sex of this animal and solving vetero-legal cases related with this species.

scholarly journals Effect of hormonal growth promotants on palatability and carcass traits of various muscles from steer and heifer carcasses from a Bos indicus - Bos taurus composite cross

2008 ◽  
Vol 48 (11) ◽  
pp. 1415 ◽  
Author(s):  
R. Watson ◽  
R. Polkinghorne ◽  
A. Gee ◽  
M. Porter ◽  
J. M. Thompson ◽  
...  
Keyword(s):  
Bos Taurus ◽  
Vastus Lateralis ◽  
Carcass Traits ◽  
Bos Indicus ◽  
Rectus Femoris ◽  
Biceps Femoris ◽  
Cooking Methods ◽  
Triceps Brachii ◽  
The Mean ◽  
Psoas Major

The effect of several different hormonal growth promotant (HGP) implant strategies on the palatability and carcass traits of different muscles in beef carcasses was investigated using samples from heifer and steer carcasses from a Bos indicus composite breed. In experiment 1, there were seven different implant strategies evaluated in heifers that were given different combinations of up to three implants (implanted at weaning, during backgrounding and at feedlot entry). A total of 112 heifers were slaughtered and 11 muscles or portions were collected from both sides [Mm. adductor femoris, gracilus, semimembranosus, longissimus dorsi lumborum, triceps brachii caput longum, semispinalis capitis, serratus ventralis cervicis, spinalis dorsi, biceps femoris (syn. gluteobiceps), tensor fasciae latae, gluteus medius (both the ‘D’ and the ‘eye’ portions) rectus femoris, vastus intermedius, vastus lateralis and vastus medialis]. These muscles were used to prepare a total of 1030 sensory samples which were aged for either 7 or 21 days and frozen. Thawed samples were cooked using different cooking methods (grill, roast and stir frying) before being evaluated by a consumer taste panel that scored samples for tenderness, juiciness, like flavour and overall liking. Experiment 2 used the steer portion from the same calving, which were treated to a similar array of HGP strategies, except that they were given up to four implants between weaning and slaughter at ~3 years of age. In experiment 2, there was a total of 12 different HGP implant strategies tested. At boning, three muscles (Mm. psoas major, longisimuss dorsi thoracis and lumborum portions) were collected from each of 79 carcasses with a total of 237 steak samples that consumers tested as grilled steaks. For both experiments, the mean of the HGP implant strategies resulted in increased ossification scores (P < 0.05) and decreased marbling scores (P < 0.05) compared with the controls, with the effect on ossification being much larger in the older steer groups. In both experiments, the different HGP strategies decreased (P < 0.05) all sensory scores compared with the controls, for all cooking method and muscle combinations. In experiment 1, there was no interaction between the mean HGP effect and muscle (P > 0.05), and aging rates differed among the muscles (P < 0.05). In experiment 2, there was a significant (P < 0.05) muscle × HGP treatment interaction, with a decrease in tenderness score due to HGP implant strategies in the M. longisimuss thoracis and lumborum portions, compared with no significant effect in the M. psoas major. For both experiments, there were no significant differences among the different implantation strategies on sensory scores (P > 0.05).

Correlation analysis of fundus autofluorescence, spectral domain optical coherence tomography, and visual function in patients with diabetic macular oedema treated with intravitreal ziv-aflibercept

2019 ◽  
Vol 29 (3) ◽  
pp. 271-277 ◽  
Author(s):  
Sergio E Hernandez-Da Mota ◽  
Enrique A Roig Melo-Granados ◽  
Jans Fromow-Guerra ◽  
Francisco Bejar-Cornejo ◽  
Roberto Gallego-Pinazo ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Regression Analysis ◽  
Correlation Analysis ◽  
Visual Function ◽  
Macular Oedema ◽  
Fundus Autofluorescence ◽  
Diabetic Macular Oedema ◽  
Optical Coherence ◽  
Average Thickness

Purpose: The aim of this study was to evaluate the correlations between fundus autofluorescence and morphologic parameters as well as visual function in patients with diabetic macular oedema treated with intravitreal ziv-aflibercept. Methods: A total of 34 eyes of 20 patients with untreated diabetic macular oedema received an intravitreal injection of ziv-aflibercept at baseline, and 1 and 2 months later. The baseline, 1-month, and two-month best corrected visual acuity determination, contrast sensitivity, spectral domain optical coherence tomography, mean central macular thickness, mean macular cube volume, mean macular cube average thickness, and fundus autofluorescence (decreased, normal, or increased; and single or multiple spots) were measured. Correlation analysis with a determination of Spearman’s rank correlation coefficient, regression analysis, agreement between investigators, and Friedman’s test were used for statistical analyses. Results: A direct correlation was observed between baseline fundus autofluorescence and macular cube average thickness at 1 month (r = 0.51, p = 0.020) and between fundus autofluorescence at 1 month and baseline macular cube average thickness (r = 0.50, p = 0.021). Regression analysis showed a coefficient of determination of 0.29 (p = 0.016) between baseline fundus autofluorescence and macular cube average thickness at 1 month. Conclusion: In patients with diabetic macular oedema, the pretreatment baseline degree of foveal fundus autofluorescence might be helpful in predicting macular cube average thickness in patients undergoing treatment with intravitreal ziv-aflibercept in the short term.

scholarly journals Determination of β3-Adrenoceptor Mediated Lipolysis in Human Fat Cells

1995 ◽  
Vol 3 (5) ◽  
pp. 447-457 ◽  
Author(s):  
Johan Hoffstedt ◽  
Manabu Shimizu ◽  
Svante Sjöstedt ◽  
Fredrik Lönnqvist
Keyword(s):  
Fat Cells

scholarly journals A new method for rapid detection of Listeria monocytogenes in food

2000 ◽  
Vol 18 (No. 3) ◽  
pp. 103-109 ◽  
Author(s):  
K. Zdeňková ◽  
K. Demnerová ◽  
G. Jeníková ◽  
J. Pazlarová
Keyword(s):  
Listeria Monocytogenes ◽  
Pcr Primers ◽  
Immunomagnetic Separation ◽  
Food Samples ◽  
Special Importance ◽  
Ground Meat ◽  
Specific Detection ◽  
Gene Encoding ◽  
Pcr Method

Listeria monocytogenes represents serious danger for human health. Thus detection of this pathogen in food, which represents its main means of entry into the organism, is a topic of special importance. The original classic methods for the determination of Listeria monocytogenes are in general laborious and time-consuming procedures. In order to address this issue we developed a new rapid method for specific detection of Listeria monocytogenes in food samples. The method consists of three steps: i) enrichment of food microflora (16 h), ii) selective isolation of Listeria sp. exploiting immunomagnetic separation (2–3 h) followed by iii) precise identification of Listeria sp. and Listeria monocytogenes using duplex PCR. PCR primers specific to part of 16S rRNA were used in order to identify the members of Listeria genus. The specific identification of Listeria monocytogenes was accomplished exploiting a pair of primers specific to gene encoding invasion-associated protein – iap (4–5 h). Amplification products, 1003 bp and 593 bp respectively, were separated by electrophoresis and visualized by UV detection. The optimized IMS-PCR method was used to test the presence of Listeria sp. and Listeria monocytogenes in food samples (ground meat, low-fat milk and cheese [olomoucké tvarůžky]). A comparison of the efficiency of the bacteria enrichment step by IMS and centrifugation was also performed. The analysis time including enrichment is less than 24 h. The detection limit for Listeria monocytogenes was found between 101–102 cfu per 25 g of food sample.

Sign in / Sign up

Export Citation Format

Share Document