Tyramide Signal Amplification for Immunoelectron Microscopy

2021 ◽  
pp. 213-222
Author(s):  
María José Ulloa-Navas ◽  
Patricia García-Tárraga ◽  
Susana González-Granero ◽  
Pedro Pérez-Borreda ◽  
Vicente Herranz-Pérez ◽  
...  
Keyword(s):  
Immunoelectron Microscopy ◽  
Signal Amplification ◽  
Tyramide Signal Amplification

Enhancement of immunohistochemical detection of HIV-1 p24 antigen in brain by tyramide signal amplification

1997 ◽  
Vol 67 (1) ◽  
pp. 103-112 ◽  
Author(s):  
Pádraig M Strappe ◽  
Ting Huei Wang ◽  
Chris Anne McKenzie ◽  
Suzanne Lowrie ◽  
Peter Simmonds ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Immunohistochemical Detection ◽  
Tyramide Signal Amplification ◽  
P24 Antigen ◽  
Hiv 1

scholarly journals Introduction of Tyramide Signal Amplification (TSA) to Pre-embedding Nanogold-Silver Staining at the Electron Microscopic Level

2005 ◽  
Vol 53 (2) ◽  
pp. 249-252 ◽  
Author(s):  
Seung-won Lee ◽  
Song Eun Lee ◽  
Seong Hyuk Ko ◽  
Eun Kyoung Hong ◽  
Kwang Il Nam ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Matrix Protein ◽  
Light And Electron Microscopy ◽  
Microscopic Level ◽  
Electron Microscopic Level ◽  
Tyramide Signal Amplification ◽  
Electron Microscopic ◽  
Simple Protocol ◽  
Tissue Antigens ◽  
A Cell

The tyramide signal amplification (TSA) technique has been shown to detect scarce tissue antigens in light and electron microscopy. In this study we applied the TSA technique at the electron microscopic level to pre-embedding immunocytochemistry. This protocol was compared to the non-amplified protocol. With the TSA protocol, the labeling of GM130, a cis-Golgi matrix protein, was tested in a cell line and found to be highly sensitive and more enhanced than that with the simple protocol. Moreover, the gold particles were well localized to the cis-side of the Golgi apparatus in both the TSA and the simple protocol.

Tyramide signal amplification of biotinylated probe in dot-blot hybridization assay for the detection of parvovirus B19 DNA in serum samples

2000 ◽  
Vol 302 (1-2) ◽  
pp. 79-87 ◽  
Author(s):  
Marialuisa Zerbini ◽  
Monica Cricca ◽  
Giovanna Gentilomi ◽  
Simona Venturoli ◽  
Giorgio Gallinella ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Parvovirus B19 ◽  
Blot Hybridization ◽  
Hybridization Assay ◽  
Tyramide Signal Amplification ◽  
Dot Blot ◽  
Serum Samples ◽  
Dot Blot Hybridization

Dual-cardiac marker capillary waveguide fluoroimmunosensor based on tyramide signal amplification

2009 ◽  
Vol 396 (3) ◽  
pp. 1187-1196 ◽  
Author(s):  
Aristeidis E. Niotis ◽  
Christos Mastichiadis ◽  
Panagiota S. Petrou ◽  
Ion Christofidis ◽  
Sotirios E. Kakabakos ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Tyramide Signal Amplification ◽  
Cardiac Marker
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