Droplet-Free Digital Enzyme-Linked Immunosorbent Assay Based on a Tyramide Signal Amplification System

2016 ◽  
Vol 88 (14) ◽  
pp. 7123-7129 ◽  
Author(s):  
Kenji Akama ◽  
Kentaro Shirai ◽  
Seigo Suzuki
Keyword(s):  
Immunosorbent Assay ◽  
Signal Amplification ◽  
Enzyme Linked Immunosorbent Assay ◽  
Tyramide Signal Amplification ◽  
Amplification System ◽  
Signal Amplification System

scholarly journals Tau in cerebrospinal fluid: A sensitive sandwich enzyme-linked immunosorbent assay using tyramide signal amplification

2007 ◽  
Vol 418 (2) ◽  
pp. 186-189 ◽  
Author(s):  
Hidenaga Yamamori ◽  
Sabiha Khatoon ◽  
Inge Grundke-Iqbal ◽  
Kaj Blennow ◽  
Michael Ewers ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Immunosorbent Assay ◽  
Signal Amplification ◽  
Enzyme Linked Immunosorbent Assay ◽  
Tyramide Signal Amplification

A dual-round signal amplification strategy for colorimetric/photoacoustic/fluorescence triple read-out detection of prostate specific antigen

2020 ◽  
Vol 56 (36) ◽  
pp. 4942-4945 ◽  
Author(s):  
Chao Jiang ◽  
Yan Huang ◽  
Ting He ◽  
Peng Huang ◽  
Jing Lin
Keyword(s):  
Prostate Specific Antigen ◽  
Immunosorbent Assay ◽  
Signal Amplification ◽  
Specific Antigen ◽  
Enzyme Linked Immunosorbent Assay ◽  
Core Shell ◽  
Elisa System ◽  
System P ◽  
Amplification Strategy

A colorimetric/fluorescence/photoacoustic triple read-out detection of prostate specific antigen (PSA) was developed by using a silica coated Au@Ag core–shell nanorod (denoted Au@Ag@SiO2) based enzyme-linked immunosorbent assay (ELISA) system.

An enzymatic signal amplification system for calorimetric studies of cellobiohydrolases

2010 ◽  
Vol 404 (2) ◽  
pp. 140-148 ◽  
Author(s):  
Leigh Murphy ◽  
Martin J. Baumann ◽  
Kim Borch ◽  
Matt Sweeney ◽  
Peter Westh
Keyword(s):  
Signal Amplification ◽  
Amplification System ◽  
Calorimetric Studies ◽  
Enzymatic Signal Amplification ◽  
Signal Amplification System

scholarly journals CRISPR/Cas13a signal amplification linked immunosorbent assay (CLISA)

2019 ◽  
Author(s):  
Qian Chen ◽  
Tian Tian ◽  
Erhu Xiong ◽  
Po Wang ◽  
Xiaoming Zhou
Keyword(s):  
Immunosorbent Assay ◽  
Signal Amplification ◽  
Limit Of Detection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Inflammatory Factor ◽  
Early Diagnosis Of Cancer ◽  
Low Concentrations ◽  
Conventional Elisa ◽  
Amplification Strategy ◽  
Endothelial Growth Factor

ABSTRACTThe enzyme-linked immunosorbent assay (ELISA) is a basic technique used in analytical and clinical investigations. However, conventional ELISA is still not sensitive enough to detect ultra-low concentrations of biomarkers for the early diagnosis of cancer, cardiovascular risk, neurological disorders, and infectious diseases. Herein we show a mechanism utilizing the CRISPR/Cas13a-based signal export amplification strategy, which double-amplifies the output signal by T7 RNA polymerase transcription and CRISPR/Cas13a collateral cleavage activity. This process is termed the CRISPR/Cas13a signal amplification linked immunosorbent assay (CLISA). The proposed method was validated by detecting an inflammatory factor, human interleukin-6 (human IL-6), and a tumor marker, human vascular endothelial growth factor (human VEGF), which achieved limit of detection (LOD) values of 45.81 fg/mL (2.29 fM) and 32.27 fg/m (0.81 fM), respectively, demonstrating that CLISA is at least 102-fold more sensitive than conventional ELISA.

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