Fast Application of Agonists to Isolated Membrane Patches

This study aims to determine the classification of mobile banking services that improve customer satisfaction based on the dimensions of E-Servqual using the Kano Model method. The classification is needed so that the banks were able to make improvements and innovations in the performance of the quality of their mobile banking services. From the results of data processing using Kano, it is known that the service attributes that need to be improved are the m-banking facility, which features a cash withdrawal service via ATM. This will have a major impact on customer satisfaction because these attributes have the highest satisfaction coefficient value of 0.67 in one-dimensional classification. Furthermore, service attributes that need to be improved and innovated are m-banking has a fast application reactivation service with a satisfaction coefficient of 0.61 in the attractive classification

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N-methyl-D-aspartate increases cytosolic Ca2+ via G proteins in cultured hippocampal neurons

AJP Cell Physiology ◽

10.1152/ajpcell.1992.262.4.c870 ◽

1992 ◽

Vol 262 (4) ◽

pp. C870-C875 ◽

Cited By ~ 13

Author(s):

K. Harada ◽

T. Yoshimura ◽

K. Nakajima ◽

H. Ito ◽

Y. Ebina ◽

...

Keyword(s):

G Proteins ◽

Hippocampal Neurons ◽

Drug Application ◽

Initial Increase ◽

Second Phase ◽

Free Solution ◽

Rat Embryos ◽

Fast Application ◽

Initial Entry ◽

Cultured Hippocampal Neurons

The changes of the cytosolic Ca2+ concentrations ([Ca2+]i) induced by N-methyl-D-aspartate (NMDA) in fura-2-loaded cultured hippocampal neurons from rat embryos were investigated by the fast application method, using a fine pipe under extracellular Mg(2+)-free conditions. In the presence of Ca2+, NMDA, at concentrations in excess of 3 microM, induced a biphasic increase of [Ca2+]i, which consisted of an initial increase with a second rise that occurred after cessation of drug application. Under Ca(2+)-free conditions, NMDA (greater than 100 microM) in the absence of glycine or NMDA (greater than 50 microM) in the presence of glycine (greater than 10 microM) induced intracellular Ca2+ mobilization, which was blocked by 30 microM 2-amino-5-phosphonovaleric acid (APV) and reduced by islet-activating protein. When the neurons were superfused with Ca(2+)-free solution, the application of 3-10 microM NMDA, which had been dissolved in Ca(2+)-containing solution, induced the second phase [Ca2+]i increase, whereas application of kainate, quisqualate, or stimulation by 50 mM K+ did not. Islet-activating protein, 1-(5-isoquinolinesulfonyl)-2-methylpiperazine dihydrochloride (H-7), and D-sphingosine reduced the second phase [Ca2+]i increase. These results suggest that NMDA-induced intracellular Ca2+ mobilization is potentiated by the initial entry of Ca2+ into the cells and is regulated in an islet-activating protein-sensitive manner.

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Research on Fast Application Layer Tree Multicast Algorithm Based on End-to-End Measurement

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.159.46 ◽

2010 ◽

Vol 159 ◽

pp. 46-50

Author(s):

Xin Hai Wang

Keyword(s):

Network Measurement ◽

Measurement Technology ◽

Application Layer ◽

Random Measurement ◽

Measurement Algorithm ◽

Covering Tree ◽

Path Measurement ◽

Simulation Results ◽

End To End ◽

Fast Application

Application Layer Multicast (ALM) is more flexible than that in IP layer and easy to optimize for specific applications, so the research on it has become a hotspot. Aiming at the problem of most ALM protocol ignoring bandwidth of covering tree, the paper presented a new heuristic algorithm Max-Delta, which inferred the underlying link topology using end-to-end measurement technology. On the basis of this, a kind of Fast Application layer Tree (FAT) algorithm to construct covering tree was proposed to meet the requirements of bandwidth. In addition, the algorithm's time complexity was also analyzed. Simulation results show that Max-Delta algorithm can obtain network topology accurately with less network measurement times comparing with random measurement algorithm and longest path measurement algorithm.

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