Solid-State Nanopores: Methods of Fabrication and Integration, and Feasibility Issues in DNA Sequencing

Nanopores ◽  
2011 ◽  
pp. 177-201 ◽  
Author(s):  
Xinsheng Sean Ling
Keyword(s):  
Solid State ◽  
Dna Sequencing

An all solid‐state near‐infrared time‐correlated single photon counting instrument for dynamic lifetime measurements in DNA sequencing applications

1996 ◽  
Vol 67 (11) ◽  
pp. 3984-3989 ◽  
Author(s):  
Benjamin L. Legendre ◽  
Daryl C. Williams ◽  
Steven A. Soper ◽  
Rainer Erdmann ◽  
Uwe Ortmann ◽  
...  
Keyword(s):  
Solid State ◽  
Dna Sequencing ◽  
Near Infrared ◽  
Single Photon ◽  
Photon Counting ◽  
Lifetime Measurements ◽  
Single Photon Counting

scholarly journals Fabrication and characterization of solid-state nanopore arrays for high-throughput DNA sequencing

2012 ◽  
Vol 23 (38) ◽  
pp. 385308 ◽  
Author(s):  
Ruby dela Torre ◽  
Joseph Larkin ◽  
Alon Singer ◽  
Amit Meller
Keyword(s):  
Solid State ◽  
Dna Sequencing ◽  
High Throughput ◽  
Nanopore Arrays ◽  
High Throughput Dna Sequencing ◽  
Fabrication And Characterization

scholarly journals Extended topological line defects in graphene for individual identification of DNA nucleobases

2020 ◽  
Vol 1 (8) ◽  
pp. 2908-2916 ◽  
Author(s):  
Rameshwar L. Kumawat ◽  
Biswarup Pathak
Keyword(s):  
Solid State ◽  
Dna Sequencing ◽  
Single Molecule ◽  
Individual Identification ◽  
Single Molecule Level ◽  
Line Defects ◽  
Dna Nucleobases

The TOC features a scheme of solid-state nanochannel-based DNA sequencing techniques. DNA nucleobases can be analyzed at the single-molecule level by adsorption on topologically extended line defects in the graphene-based electrode setup.

scholarly journals Solid-State Nanopore-Based DNA Sequencing Technology

2016 ◽  
Vol 2016 ◽  
pp. 1-13 ◽  
Author(s):  
Zewen Liu ◽  
Yifan Wang ◽  
Tao Deng ◽  
Qi Chen
Keyword(s):  
Solid State ◽  
Dna Sequencing ◽  
Surface Properties ◽  
Dna Molecules ◽  
Sequencing Technology ◽  
Gene Detection ◽  
High Quality ◽  
Translocation Velocity ◽  
And Behavior

The solid-state nanopore-based DNA sequencing technology is becoming more and more attractive for its brand new future in gene detection field. The challenges that need to be addressed are diverse: the effective methods to detect base-specific signatures, the control of the nanopore’s size and surface properties, and the modulation of translocation velocity and behavior of the DNA molecules. Among these challenges, the realization of the high-quality nanopores with the help of modern micro/nanofabrication technologies is a crucial one. In this paper, typical technologies applied in the field of solid-state nanopore-based DNA sequencing have been reviewed.

Solid-State Nanopore System for Label-free DNA Sequencing

2016 ◽  
Author(s):  
Y. Yanagawa ◽  
I. Yanagi ◽  
R. Akahori ◽  
T. Iwasaki ◽  
Y. Goto ◽  
...  
Keyword(s):  
Solid State ◽  
Dna Sequencing ◽  
Label Free ◽  
Free Dna

scholarly journals Progress toward Ultrafast DNA Sequencing Using Solid-State Nanopores

2007 ◽  
Vol 53 (11) ◽  
pp. 1996-2001 ◽  
Author(s):  
Gautam V Soni ◽  
Amit Meller
Keyword(s):  
Solid State ◽  
Dna Sequencing ◽  
Dna Sequence ◽  
Large Scale ◽  
Optical Detection ◽  
Ionic Current ◽  
Molecular Beacons ◽  
Single Nucleotide ◽  
Dual Color ◽  
Target Dna

Abstract Background: Measurements of the ionic current flowing through nanometer-scale pores (nanopores) have been used to analyze single DNA and RNA molecules, with the ultimate goal of achieving ultrafast DNA sequencing. However, attempts at purely electronic measurements have not achieved the signal contrast required for single nucleotide differentiation. In this report we propose a novel method of optical detection of DNA sequence translocating through a nanopore. Methods: Each base of the target DNA sequence is 1st mapped onto a 2-unit code, 2 10-bp nucleotide sequence, by biochemical conversion into Designed DNA Polymers. These 2-unit codes are then hybridized to complementary, fluorescently labeled, and self-quenching molecular beacons. As the molecular beacons are sequentially unzipped during translocation through a <2-nm-wide nanopore, their fluorescent tags are unquenched and are detected by a custom-built dual-color total internal reflection fluorescence (TIRF) microscope. The 2-color optical signal is then correlated to the target DNA sequence. Results: A dual-color TIRFM microscope with single-molecule resolution was constructed, and controlled fabrication of 1-dimensional and 2-dimensional arrays of solid-state nanopores was performed. A nanofluidic cell assembly was constructed for TIRF-based optical detection of voltage-driven DNA translocation through a nanopore. Conclusions: We present a novel nanopore-based DNA sequencing technique that uses an optical readout of DNA translocating unzipping through a nanopore. Our technique offers better single nucleotide differentiation in sequence readout, as well as the possibility of large-scale parallelism using nanopore arrays.

scholarly journals DNA sequencing and bar-coding using solid-state nanopores

2012 ◽  
Vol 33 (23) ◽  
pp. 3437-3447 ◽  
Author(s):  
Evrim Atas ◽  
Alon Singer ◽  
Amit Meller
Keyword(s):  
Solid State ◽  
Dna Sequencing

Solid-state nanopores for biosensing with submolecular resolution

2012 ◽  
Vol 40 (4) ◽  
pp. 624-628 ◽  
Author(s):  
Azadeh Bahrami ◽  
Fatma Doğan ◽  
Deanpen Japrung ◽  
Tim Albrecht
Keyword(s):  
Solid State ◽  
Dna Sequencing ◽  
Single Molecule ◽  
Dna Interactions ◽  
Patch Clamp Technique ◽  
New Class ◽  
Protein Dna Interactions ◽  
Transmembrane Pores ◽  
Recent Developments ◽  
Detection Of Biomolecules

Biological cell membranes contain various types of ion channels and transmembrane pores in the 1–100 nm range, which are vital for cellular function. Individual channels can be probed electrically, as demonstrated by Neher and Sakmann in 1976 using the patch-clamp technique [Neher and Sakmann (1976) Nature 260, 799–802]. Since the 1990s, this work has inspired the use of protein or solid-state nanopores as inexpensive and ultrafast sensors for the detection of biomolecules, including DNA, RNA and proteins, but with particular focus on DNA sequencing. Solid-state nanopores in particular have the advantage that the pore size can be tailored to the analyte in question and that they can be modified using semi-conductor processing technology. This establishes solid-state nanopores as a new class of single-molecule biosensor devices, in some cases with submolecular resolution. In the present review, we discuss a few of the most important recent developments in this field and how they might be applied to studying protein–protein and protein–DNA interactions or in the context of ultra-fast DNA sequencing.

Sign in / Sign up

Export Citation Format

Share Document