Bacillus stearothermophilus for Thermophilic Production of l-Lactic Acid

The need for safe food products amid increasingly perceived widespread use of various foodadditives which are sometimes hazardous to health. Preservative is one of food additives intendedto extend the shelf life of food products. The preservative produced by lactic acid bacteria isbacteriocin. Nisin, one of bacteriocin produced by LAB Lactococcus lactis,is considered safeand allowed its use in many countries, including Indonesia. This preservative is widely used forfood products due to its ability in inhibiting bacteria, especially Gram-positive bacteria such asClostridium botulinum, Staphylococcus aureus, Streptococcus hemolyticus, Listeriamonocytogenes, Bacillus stearothermophilus, and Bacillus subtilis.Nisin works actively at lowpH and can be used singly or in combination with other preservation treatments. Based on itsphysico chemical properties and bioactivity, the use of nisin in fishery products is more suitable infermentation process as well as fish canned in acidic medium such as tomato sauce.

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Demonstration of Retinal Glycogen with Silver Methenamine

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100066875 ◽

1971 ◽

Vol 29 ◽

pp. 564-565

Author(s):

A. W. Sedar ◽

G. H. Bresnick

Keyword(s):

Lactic Acid ◽

Chromic Acid ◽

Periodic Acid ◽

Müller Cell ◽

External Limiting Membrane ◽

Electron Microscope Level ◽

Reactive Process ◽

Inner Limiting Membrane ◽

Muller Cell ◽

And Migration

After experimetnal damage to the retina with a variety of procedures Müller cell hypertrophy and migration occurs. According to Kuwabara and others the reactive process in these injuries is evidenced by a marked increase in amount of glycogen in the Müller cells. These cells were considered originally supporting elements with fiber processes extending throughout the retina from inner limiting membrane to external limiting membrane, but are known now to have high lactic acid dehydrogenase activity and the ability to synthesize glycogen. Since the periodic acid-chromic acid-silver methenamine technique was shown to demonstrate glycogen at the electron microscope level, it was selected to react with glycogen in the fine processes of the Müller cell that ramify among the neural elements in various layers of the retina and demarcate these cells cytologically. The Rhesus monkey was chosen as an example of a well vascularized retina and the rabbit as an example of a avascular retina to explore the possibilities of the technique.

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