Structure of the Escherichia Coli ATP Synthase from Electron Microscopy Studies

1989 ◽  
pp. 107-114
Author(s):  
Uwe Lucken ◽  
Edward P. Gogol ◽  
Roderick A. Capaldi
Keyword(s):  
Electron Microscopy ◽  
Escherichia Coli ◽  
Atp Synthase

Cross-linking and electron microscopy studies of the structure and functioning of the Escherichia coli ATP synthase

2000 ◽  
Vol 203 (1) ◽  
pp. 29-33 ◽  
Author(s):  
R.A. Capaldi ◽  
B. Schulenberg ◽  
J. Murray ◽  
R. Aggeler
Keyword(s):  
Electron Microscopy ◽  
Escherichia Coli ◽  
Oxidative Phosphorylation ◽  
Atp Synthase ◽  
Proton Gradient ◽  
Cross Linking ◽  
Combination Of Methods ◽  
Chemical Cross Linking

ATP synthase, also called F(1)F(o)-ATPase, catalyzes the synthesis of ATP during oxidative phosphorylation. The enzyme is reversible and is able to use ATP to drive a proton gradient for transport purposes. Our work has focused on the enzyme from Escherichia coli (ECF(1)F(o)). We have used a combination of methods to study this enzyme, including electron microscopy and chemical cross-linking. The utility of these two approaches in particular, and the important insights they give into the structure and mechanism of the ATP synthase, are reviewed.

Structural organization of escherichia coli ribosomes as determined by immuno electron microscopy

1978 ◽  
Vol 36 (2) ◽  
pp. 166-167 ◽  
Author(s):  
G. Stöffler ◽  
R.W. Bald ◽  
J. Dieckhoff ◽  
H. Eckhard ◽  
R. Lührmann ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Escherichia Coli ◽  
Ribosomal Proteins ◽  
Structural Organization ◽  
Three Dimensional ◽  
Ribosomal Subunit ◽  
Spatial Arrangement ◽  
Small Subunit ◽  
Antibody Binding ◽  
Immuno Electron Microscopy

A central step towards an understanding of the structure and function of the Escherichia coli ribosome, a large multicomponent assembly, is the elucidation of the spatial arrangement of its 54 proteins and its three rRNA molecules. The structural organization of ribosomal components has been investigated by a number of experimental approaches. Specific antibodies directed against each of the 54 ribosomal proteins of Escherichia coli have been performed to examine antibody-subunit complexes by electron microscopy. The position of the bound antibody, specific for a particular protein, can be determined; it indicates the location of the corresponding protein on the ribosomal surface.The three-dimensional distribution of each of the 21 small subunit proteins on the ribosomal surface has been determined by immuno electron microscopy: the 21 proteins have been found exposed with altogether 43 antibody binding sites. Each one of 12 proteins showed antibody binding at remote positions on the subunit surface, indicating highly extended conformations of the proteins concerned within the 30S ribosomal subunit; the remaining proteins are, however, not necessarily globular in shape (Fig. 1).

The nucleotide binding affinities of two critical conformations of Escherichia coli ATP synthase

2021 ◽  
pp. 108899
Author(s):  
Yunxiang Li ◽  
Neydy A. Valdez ◽  
Nelli Mnatsakanyan ◽  
Joachim Weber
Keyword(s):  
Escherichia Coli ◽  
Atp Synthase ◽  
Nucleotide Binding ◽  
Binding Affinities

scholarly journals Mutagenesis of subunit delta from Escherichia coli F1F0-ATP synthase.

1994 ◽  
Vol 269 (1) ◽  
pp. 418-426
Author(s):  
A.L. Hazard ◽  
A.E. Senior
Keyword(s):  
Escherichia Coli ◽  
Atp Synthase ◽  
F1f0 Atp Synthase

Escherichia coli 70 S ribosome at 15 Å resolution by cryo-electron microscopy: localization of fmet-tRNAfMet and fitting of L1 protein

1998 ◽  
Vol 280 (1) ◽  
pp. 103-116 ◽  
Author(s):  
Arun Malhotra ◽  
Pawel Penczek ◽  
Rajendra K Agrawal ◽  
Irene S Gabashvili ◽  
Robert A Grassucci ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Escherichia Coli ◽  
Cryo Electron Microscopy ◽  
L1 Protein

scholarly journals The Regulatory C-Terminal Domain of Subunit   of FoF1 ATP Synthase Is Dispensable for Growth and Survival of Escherichia coli

2011 ◽  
Vol 193 (8) ◽  
pp. 2046-2052 ◽  
Author(s):  
N. Taniguchi ◽  
T. Suzuki ◽  
M. Berney ◽  
M. Yoshida ◽  
G. M. Cook
Keyword(s):  
Escherichia Coli ◽  
Atp Synthase ◽  
Growth And Survival ◽  
Terminal Domain ◽  
Fof1 Atp Synthase
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