Development of a DNA Transfer System for Pines

1988 ◽  
pp. 231-241 ◽  
Author(s):  
Anne-Marie Stomp ◽  
Carol Loopstra ◽  
Ronald Sederoff ◽  
Scott Chilton ◽  
JoAnne Fillatti ◽  
...  
Keyword(s):  
Dna Transfer ◽  
Transfer System

scholarly journals Functional and Mutational Analysis of Conjugative Transfer Region 1 (Tra1) from the IncHI1 Plasmid R27

2002 ◽  
Vol 184 (8) ◽  
pp. 2173-2180 ◽  
Author(s):  
Trevor D. Lawley ◽  
Matthew W. Gilmour ◽  
James E. Gunton ◽  
Leah J. Standeven ◽  
Diane E. Taylor
Keyword(s):  
Leucine Zipper ◽  
Mutational Analysis ◽  
Dna Transfer ◽  
Genetic Complementation ◽  
Conjugative Transfer ◽  
Transfer System ◽  
Sequence Motifs ◽  
Specific Phage ◽  
Transfer Region ◽  
Coupling Protein

ABSTRACT The conjugative transfer region 1 (Tra1) of the IncHI1 plasmid R27 was subjected to DNA sequence analysis, mutagenesis, genetic complementation, and an H-pilus-specific phage assay. Analysis of the nucleotide sequence indicated that the Tra1 region contains genes coding for mating pair formation (Mpf) and DNA transfer replication (Dtr) and a coupling protein. Insertional disruptions of 9 of the 14 open reading frames (ORFs) in the Tra1 region resulted in a transfer-deficient phenotype. Conjugative transfer was restored for each transfer mutant by genetic complementation. An intergenic region between traH and trhR was cloned and mobilized by R27, indicating the presence of an origin of transfer (oriT). The five ORFs immediately downstream of the oriT region are involved in H-pilus production, as determined by an H-pilus-specific phage assay. Three of these ORFs encode proteins homologous to Mpf proteins from IncF plasmids. Upstream of the oriT region are four ORFs required for plasmid transfer but not H-pilus production. TraI contains sequence motifs that are characteristic of relaxases from the IncP lineage but share no overall homology to known relaxases. TraJ contains both an Arc repressor motif and a leucine zipper motif. A putative coupling protein, TraG, shares a low level of homology to the TraG family of coupling proteins and contains motifs that are important for DNA transfer. This analysis indicates that the Mpf components of R27 share a common lineage with those of the IncF transfer system, whereas the relaxase of R27 is ancestrally related to that of the IncP transfer system.

scholarly journals TraG from RP4 and TraG and VirD4 from Ti Plasmids Confer Relaxosome Specificity to the Conjugal Transfer System of pTiC58

2000 ◽  
Vol 182 (6) ◽  
pp. 1541-1548 ◽  
Author(s):  
Claire M. Hamilton ◽  
Hyewon Lee ◽  
Pei-Li Li ◽  
David M. Cook ◽  
Kevin R. Piper ◽  
...  
Keyword(s):  
Mating Systems ◽  
Pair Formation ◽  
Ti Plasmid ◽  
Dna Transfer ◽  
Conjugal Transfer ◽  
Transfer System ◽  
Essential Factor ◽  
Ti Plasmids ◽  
Coupling Protein ◽  
Formation System

ABSTRACT Plasmid conjugation systems are composed of two components, the DNA transfer and replication system, or Dtr, and the mating pair formation system, or Mpf. During conjugal transfer an essential factor, called the coupling protein, is thought to interface the Dtr, in the form of the relaxosome, with the Mpf, in the form of the mating bridge. These proteins, such as TraG from the IncP1 plasmid RP4 (TraGRP4) and TraG and VirD4 from the conjugal transfer and T-DNA transfer systems of Ti plasmids, are believed to dictate specificity of the interactions that can occur between different Dtr and Mpf components. The Ti plasmids of Agrobacterium tumefaciens do not mobilize vectors containing the oriT of RP4, but these IncP1 plasmid derivatives lack the trans-acting Dtr functions and TraGRP4. A. tumefaciensdonors transferred a chimeric plasmid that contains theoriT and Dtr genes of RP4 and the Mpf genes of pTiC58, indicating that the Ti plasmid mating bridge can interact with the RP4 relaxosome. However, the Ti plasmid did not mobilize transfer from an IncQ relaxosome. The Ti plasmid did mobilize such plasmids if TraGRP4 was expressed in the donors. Mutations intraG RP4 with defined effects on the RP4 transfer system exhibited similar phenotypes for Ti plasmid-mediated mobilization of the IncQ vector. When provided with VirD4, thetra system of pTiC58 mobilized plasmids from the IncQ relaxosome. However, neither TraGRP4 nor VirD4 restored transfer to a traG mutant of the Ti plasmid. VirD4 also failed to complement a traG RP4 mutant for transfer from the RP4 relaxosome or for RP4-mediated mobilization from the IncQ relaxosome. TraGRP4-mediated mobilization of the IncQ plasmid by pTiC58 did not inhibit Ti plasmid transfer, suggesting that the relaxosomes of the two plasmids do not compete for the same mating bridge. We conclude that TraGRP4 and VirD4 couples the IncQ but not the Ti plasmid relaxosome to the Ti plasmid mating bridge. However, VirD4 cannot couple the IncP1 or the IncQ relaxosome to the RP4 mating bridge. These results support a model in which the coupling proteins specify the interactions between Dtr and Mpf components of mating systems.

Evaluation and Optimization of a DNA Transfer System forEdwardsiella ictaluri

2001 ◽  
Vol 13 (2) ◽  
pp. 163-167 ◽  
Author(s):  
Kirk J. Maurer ◽  
Mark L. Lawrence ◽  
Denise H. Fernandez ◽  
Ronald L. Thune
Keyword(s):  
Dna Transfer ◽  
Transfer System

Development of an efficient conjugal DNA transfer system between Escherichia coli and a non-sporulating Streptomyces strain

2018 ◽  
Vol 144 ◽  
pp. 60-66 ◽  
Author(s):  
Diana Rocha ◽  
Beatriz Ruiz-Villafán ◽  
Monserrat Manzo ◽  
Romina Rodríguez-Sanoja ◽  
Sergio Sánchez
Keyword(s):  
Escherichia Coli ◽  
Dna Transfer ◽  
Transfer System ◽  
Streptomyces Strain

scholarly journals Interaction between the co-inherited TraG coupling protein and the TraJ membrane-associated protein of the H-plasmid conjugative DNA transfer system resembles chromosomal DNA translocases

Microbiology ◽  
2007 ◽  
Vol 153 (2) ◽  
pp. 428-441 ◽  
Author(s):  
James E Gunton ◽  
Matthew W Gilmour ◽  
Kelly P Baptista ◽  
Trevor D Lawley ◽  
Diane E Taylor
Keyword(s):  
Dna Transfer ◽  
Transfer System ◽  
Chromosomal Dna ◽  
Dna Translocases ◽  
Coupling Protein

Stability in the informal value transfer systems: norms of trust or institutions of control?

2017 ◽  
pp. 120-130
Author(s):  
A. Lyasko
Keyword(s):  
Social Control ◽  
Institutional Economics ◽  
Theoretic Model ◽  
Transfer System ◽  
Value Transfer ◽  
Partner Interaction ◽  
Game Theoretic ◽  
Game Theoretic Model ◽  
Informal Value Transfer System

Informal financial operations exist in the shadow of official regulation and cannot be protected by the formal legal instruments, therefore raising concerns about the enforcement of obligations taken by their participants. This paper analyzes two alternative types of auxiliary institutions, which can coordinate expectations of the members of informal value transfer systems, namely attitudes of trust and norms of social control. It offers some preliminary approaches to creating a game-theoretic model of partner interaction in the informal value transfer system. It also sheds light on the perspectives of further studies in this area of institutional economics.

What Price Freedom? Implications for Scottish football of the potential abolition of the transfer system

1996 ◽  
Vol 15 (First Serie (1) ◽  
pp. 83-100 ◽  
Author(s):  
Stephen Morrow
Keyword(s):  
Transfer System
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