Steroid hormone regulation of cultured breast cancer cells

1988 ◽  
pp. 307-341 ◽  
Author(s):  
Philippa D. Darbre ◽  
Roger J. B. King
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Steroid Hormone ◽  
Hormone Regulation

scholarly journals Estrogen Regulation of the Glucuronidation Enzyme UGT2B15 in Estrogen Receptor-Positive Breast Cancer Cells

Endocrinology ◽  
2006 ◽  
Vol 147 (8) ◽  
pp. 3843-3850 ◽  
Author(s):  
William R. Harrington ◽  
Surojeet Sengupta ◽  
Benita S. Katzenellenbogen
Keyword(s):  
Breast Cancer ◽  
Estrogen Receptor ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Steroid Hormone ◽  
Transcriptional Response ◽  
Mrna Levels ◽  
Dependent Manner ◽  
Estrogen Regulation ◽  
Estradiol Stimulation

Estrogens and androgens influence many properties of breast cancer cells; hence, regulation of local estrogen and androgen levels by enzymes involved in steroid hormone biosynthesis and metabolism would impact signaling by these hormones in breast cancer cells. In this study, we show that the UDP-glucuronosyltransferase (UGT) enzyme UGT2B15, a member of the UGT family of phase II enzymes involved in the glucuronidation of steroids and xenobiotics, is a novel, estrogen-regulated gene in estrogen receptor (ER)-positive human breast cancer cells (MCF-7, BT474, T47D, and ZR-75). UGT2B15 is the only UGT2B enzyme up-regulated by estrogen, and marked estradiol stimulation of UGT2B15 mRNA levels is observed, in a time- and dose-dependent manner. UGT2B15 stimulation by estradiol is blocked by the antiestrogen ICI182,780, but not by the translational inhibitor cycloheximide, indicating that UGT2B15 is likely a primary transcriptional response mediated through the ER. UGT2B15 up-regulation is also evoked by other estrogens (propylpyrazoletriol, genistein) and by the androgen 5α-dihydrotestosterone working through the ER, but not by other steroid hormone receptor ligands. Western blot and immunocytochemical analyses with several UGT2B-specific antibodies we have designed and steroid glucuronidation assays indicate a large increase in both cellular UGT2B15 protein and enzyme activity after estrogen treatment. Due to the important role of UGT enzymes in forming conjugates between steroids and glucuronic acid, thereby inactivating them and targeting them for removal, the estrogen-induced up-regulation of UGT2B15 might have a significant moderating effect on estrogen and androgen concentrations, thereby reducing their signaling in breast cancer cells.

scholarly journals Steroid hormone receptors and coregulators in endocrine-resistant and estrogen-independent breast cancer cells

2005 ◽  
Vol 118 (4) ◽  
pp. 832-840 ◽  
Author(s):  
Nanna Sarvilinna ◽  
Heli Eronen ◽  
Susanna Miettinen ◽  
Annika Vienonen ◽  
Timo Ylikomi
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Hormone Receptors ◽  
Steroid Hormone ◽  
Steroid Hormone Receptors

The proliferation of breast cancer cells are inhibited by the human intrabody targeting cyclinD1

2010 ◽  
Vol 34 (8) ◽  
pp. S49-S49
Author(s):  
Lei Wang ◽  
Xun Zhou ◽  
Lihong Zhou ◽  
Yong Chen ◽  
Xun Zhu ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Breast Cancer Cells
Sign in / Sign up

Export Citation Format

Share Document