Higher-Order Structure and Protein Aggregate Characterization of Protein Therapeutics: Perspectives from Good Manufacturing Practices and Regulatory Guidance

2013 ◽  
pp. 261-281
Author(s):  
Evi B. Struble ◽  
John F. Cipollo ◽  
Chava Kimchi-Sarfaty ◽  
Zuben E. Sauna ◽  
Jack A. Ragheb ◽  
...  
Keyword(s):  
Higher Order ◽  
Protein Therapeutics ◽  
Order Structure ◽  
Protein Aggregate ◽  
Regulatory Guidance ◽  
Good Manufacturing Practices

scholarly journals Chemometric outlier classification of 2D-NMR spectra to enable higher order structure characterization of protein therapeutics

2020 ◽  
Vol 199 ◽  
pp. 103973 ◽  
Author(s):  
David A. Sheen ◽  
Vincent K. Shen ◽  
Robert G. Brinson ◽  
Luke W. Arbogast ◽  
John P. Marino ◽  
...  
Keyword(s):  
Nmr Spectra ◽  
2D Nmr ◽  
Higher Order ◽  
Protein Therapeutics ◽  
Structure Characterization ◽  
Order Structure ◽  
2D Nmr Spectra

Characterization of Protein Higher Order Structure Using Vibrational Circular Dichroism Spectroscopy

2013 ◽  
Vol 14 (2) ◽  
pp. 199-208 ◽  
Author(s):  
Radhika P. Nagarkar ◽  
Brian M. Murphy ◽  
Xiaotong Yu ◽  
Mark Cornell Manning ◽  
Wasfi A. Al-Azzam
Keyword(s):  
Circular Dichroism ◽  
Higher Order ◽  
Circular Dichroism Spectroscopy ◽  
Vibrational Circular Dichroism ◽  
Order Structure ◽  
Circular Dichroïsm

Characterization of nucleic acid higher order structure by gas-phase H/D exchange in a quadrupole-FT-ICR mass spectrometer

Biopolymers ◽  
2009 ◽  
Vol 91 (4) ◽  
pp. 256-264 ◽  
Author(s):  
Jingjie Mo ◽  
Gabrielle C. Todd ◽  
Kristina Håkansson
Keyword(s):  
Nucleic Acid ◽  
Gas Phase ◽  
Mass Spectrometer ◽  
Higher Order ◽  
Order Structure ◽  
Ft Icr

scholarly journals CENP-N promotes the compaction of centromeric chromatin

2021 ◽  
Author(s):  
Keda Zhou ◽  
Magdalena Gebala ◽  
Dustin C Woods ◽  
Kousik Sundararajan ◽  
Garrett Edwards ◽  
...  
Keyword(s):  
Chromatin Structure ◽  
Higher Order ◽  
Histone Variant ◽  
Order Structure ◽  
Interacting Protein ◽  
Biophysical Characterization ◽  
Centromeric Chromatin

The histone variant CENP-A is the epigenetic determinant for the centromere, where it is interspersed with canonical H3 to form a specialized chromatin structure that nucleates the kinetochore. The arrangement of nucleosomes at the centromere into higher order structure is unknown. Here we demonstrate that the CENP-A interacting protein CENP-N promotes the stacking of CENP-A containing mono-nucleosomes and nucleosomal arrays through a previously undefined interaction between the α6 helix of CENP-N with the DNA of a neighboring nucleosome. We describe the cryoEM structures and biophysical characterization of such CENP-N mediated nucleosome stacks and nucleosomal arrays and demonstrate that this interaction is responsible for the formation of densely packed chromatin at the centromere in the cell. Our results provide first evidence that CENP-A, together with CENP-N, promotes specific chromatin higher order structure at the centromere.

Sign in / Sign up

Export Citation Format

Share Document