Does Human Lactoferrin in the Milk of Transgenic Mice Deliver Iron to Suckling Neonates?

2001 ◽  
pp. 233-239 ◽  
Author(s):  
Linda H. Hanson ◽  
Valerie Sawicki ◽  
Andrew Lewis ◽  
Jan H. Nuijens ◽  
Margaret C. Neville ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Human Lactoferrin

A mWAP–hLF hybrid gene locus gave extremely high level expression of human lactoferrin in the milk of transgenic mice

2009 ◽  
Vol 18 (4) ◽  
pp. 573-582 ◽  
Author(s):  
Gengshou Shi ◽  
Hongxing Chen ◽  
Xiaojie Wu ◽  
Yanrong Zhou ◽  
Zhuguo Liu ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Gene Locus ◽  
Human Lactoferrin ◽  
High Level Expression ◽  
Hybrid Gene ◽  
High Level

High-Level Expression of Human Lactoferrin in Milk of Transgenic Mice Using Genomic Lactoferrin Sequence

1999 ◽  
Vol 126 (2) ◽  
pp. 320-325 ◽  
Author(s):  
S. J. Kim ◽  
B. H. Sohn ◽  
S. Jeong ◽  
K.-W. Pak ◽  
J.-S. Park ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Human Lactoferrin ◽  
High Level Expression ◽  
High Level

Purification and antibacterial activity of recombinant human lactoferrin in milk of transgenic mice

2009 ◽  
Vol 6 (3) ◽  
pp. 253-256
Author(s):  
Miao Ming-Xing ◽  
Yuan Yu-Guo ◽  
An Li-You ◽  
Zhao Jun-Hui ◽  
Bai Ya-Jun ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Transgenic Mice ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Gel Filtration ◽  
Human Lactoferrin ◽  
Diffusion Method ◽  
Enzyme Linked Immunosorbent Assay ◽  
Recombinant Human Lactoferrin ◽  
Agar Disc

AbstractTo verify its antibacterial activity, recombinant human lactoferrin (rhLF) was extracted from the milk of transgenic mice (Mus musculus) (PCL25and AP) by gel filtration chromatography and analysed by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE), Western blot and enzyme-linked immunosorbent assay (ELISA). In addition, its bacteriostatic properties were tested using the agar disc diffusion method. ELISA analysis showed that the concentration of rhLF in the milk of transgenic mice ranged from 7 to 8 mg/ml, and the recombinant protein expressed in the milk had the same molecular weight as the native protein (~78 kDa), indicating that the rhLFs had a strong antibacterial activity onEscherichia coliandSalmonella.

The state of health and the reproductive potential of transgenic mice secreting recombinant human lactoferrin in milk

2009 ◽  
Vol 427 (1) ◽  
pp. 195-198 ◽  
Author(s):  
A. V. Deykin ◽  
T. G. Ermolkevich ◽  
Ya. G. Gursky ◽  
A. N. Krasnov ◽  
S. G. Georgieva ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Reproductive Potential ◽  
The State ◽  
Human Lactoferrin ◽  
State Of Health ◽  
Recombinant Human Lactoferrin

Expression of human lactoferrin in milk of transgenic mice

1994 ◽  
Vol 3 (2) ◽  
pp. 99-108 ◽  
Author(s):  
Gerard J. Platenburg ◽  
Erika P. A. Kootwijk ◽  
Patricia M. Kooiman ◽  
Shelley L. Woloshuk ◽  
Jan H. Nuijens ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Human Lactoferrin

A study of recombinant human lactoferrin secreted in milk of transgenic mice

2006 ◽  
Vol 411 (1) ◽  
pp. 336-338 ◽  
Author(s):  
A. V. Sokolov ◽  
M. O. Pulina ◽  
A. V. Kristiyan ◽  
E. T. Zakharova ◽  
O. L. Runova ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Human Lactoferrin ◽  
Recombinant Human Lactoferrin

scholarly journals Characterization of Recombinant Human Lactoferrin Secreted in Milk of Transgenic Mice

1997 ◽  
Vol 272 (13) ◽  
pp. 8802-8807 ◽  
Author(s):  
Jan H. Nuijens ◽  
Patrick H. C. van Berkel ◽  
Marlieke E. J. Geerts ◽  
Peter Paul Hartevelt ◽  
Herman A. de Boer ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Human Lactoferrin ◽  
Recombinant Human Lactoferrin

scholarly journals Expression Analysis of a Bovine .BETA.-Casein/Human Lactoferrin Hybrid Gene in Transgenic Mice.

1997 ◽  
Vol 43 (2) ◽  
pp. 143-149 ◽  
Author(s):  
Sun Jung KIM ◽  
Ko-Woon LEE ◽  
Dae-Yeul YU ◽  
Yong-Mahn HAN ◽  
Chul-Sang LEE ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Expression Analysis ◽  
Human Lactoferrin ◽  
Hybrid Gene ◽  
Beta Casein
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