scholarly journals Characterization of Recombinant Human Lactoferrin Secreted in Milk of Transgenic Mice

1997 ◽  
Vol 272 (13) ◽  
pp. 8802-8807 ◽  
Author(s):  
Jan H. Nuijens ◽  
Patrick H. C. van Berkel ◽  
Marlieke E. J. Geerts ◽  
Peter Paul Hartevelt ◽  
Herman A. de Boer ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Human Lactoferrin ◽  
Recombinant Human Lactoferrin

scholarly journals Characterization of recombinant human lactoferrin N-glycans expressed in the milk of transgenic cows

PLoS ONE ◽  
2017 ◽  
Vol 12 (2) ◽  
pp. e0171477 ◽  
Author(s):  
Annabelle Le Parc ◽  
Sercan Karav ◽  
Camille Rouquié ◽  
Elizabeth A. Maga ◽  
Apichaya Bunyatratchata ◽  
...  
Keyword(s):  
Human Lactoferrin ◽  
Recombinant Human Lactoferrin ◽  
Transgenic Cows

Purification and antibacterial activity of recombinant human lactoferrin in milk of transgenic mice

2009 ◽  
Vol 6 (3) ◽  
pp. 253-256
Author(s):  
Miao Ming-Xing ◽  
Yuan Yu-Guo ◽  
An Li-You ◽  
Zhao Jun-Hui ◽  
Bai Ya-Jun ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Transgenic Mice ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Gel Filtration ◽  
Human Lactoferrin ◽  
Diffusion Method ◽  
Enzyme Linked Immunosorbent Assay ◽  
Recombinant Human Lactoferrin ◽  
Agar Disc

AbstractTo verify its antibacterial activity, recombinant human lactoferrin (rhLF) was extracted from the milk of transgenic mice (Mus musculus) (PCL25and AP) by gel filtration chromatography and analysed by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE), Western blot and enzyme-linked immunosorbent assay (ELISA). In addition, its bacteriostatic properties were tested using the agar disc diffusion method. ELISA analysis showed that the concentration of rhLF in the milk of transgenic mice ranged from 7 to 8 mg/ml, and the recombinant protein expressed in the milk had the same molecular weight as the native protein (~78 kDa), indicating that the rhLFs had a strong antibacterial activity onEscherichia coliandSalmonella.

The state of health and the reproductive potential of transgenic mice secreting recombinant human lactoferrin in milk

2009 ◽  
Vol 427 (1) ◽  
pp. 195-198 ◽  
Author(s):  
A. V. Deykin ◽  
T. G. Ermolkevich ◽  
Ya. G. Gursky ◽  
A. N. Krasnov ◽  
S. G. Georgieva ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Reproductive Potential ◽  
The State ◽  
Human Lactoferrin ◽  
State Of Health ◽  
Recombinant Human Lactoferrin

A study of recombinant human lactoferrin secreted in milk of transgenic mice

2006 ◽  
Vol 411 (1) ◽  
pp. 336-338 ◽  
Author(s):  
A. V. Sokolov ◽  
M. O. Pulina ◽  
A. V. Kristiyan ◽  
E. T. Zakharova ◽  
O. L. Runova ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Human Lactoferrin ◽  
Recombinant Human Lactoferrin

Production and Characterization of Recombinant Human Lactoferrin in Transgenic Javanica Rice

2005 ◽  
Vol 55 (2) ◽  
pp. 213-222 ◽  
Author(s):  
Diah Rachmawati ◽  
Takuya Mori ◽  
Takehiko Hosaka ◽  
Fumio Takaiwa ◽  
Eiichi Inoue ◽  
...  
Keyword(s):  
Human Lactoferrin ◽  
Recombinant Human Lactoferrin

scholarly journals Comprehensive characterization of the site-specific N-glycosylation of wild-type and recombinant human lactoferrin expressed in the milk of transgenic cloned cattle

Glycobiology ◽  
2010 ◽  
Vol 21 (2) ◽  
pp. 206-224 ◽  
Author(s):  
Tian Yu ◽  
Chengdong Guo ◽  
Jianwu Wang ◽  
Piliang Hao ◽  
Shunchao Sui ◽  
...  
Keyword(s):  
Human Lactoferrin ◽  
Wild Type ◽  
Site Specific ◽  
Recombinant Human Lactoferrin ◽  
Comprehensive Characterization

Expression and characterization of recombinant human lactoferrin in edible alga Chlamydomonas reinhardtii

2019 ◽  
Vol 83 (5) ◽  
pp. 851-859 ◽  
Author(s):  
Xiaonan Pang ◽  
Yuxi Tong ◽  
Wenzhi Xue ◽  
Yi-feng Yang ◽  
Xiwen Chen ◽  
...  
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Human Lactoferrin ◽  
Recombinant Human Lactoferrin
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