Human Lactoferrin in the Milk of Transgenic Mice Increases Intestinal Growth in Ten-Day-Old Suckling Neonates

2001 ◽  
pp. 107-113 ◽  
Author(s):  
P. Zhang ◽  
V. Sawicki ◽  
A. Lewis ◽  
L. Hanson ◽  
J. H. Nuijens ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Human Lactoferrin ◽  
Intestinal Growth

Does Human Lactoferrin in the Milk of Transgenic Mice Deliver Iron to Suckling Neonates?

2001 ◽  
pp. 233-239 ◽  
Author(s):  
Linda H. Hanson ◽  
Valerie Sawicki ◽  
Andrew Lewis ◽  
Jan H. Nuijens ◽  
Margaret C. Neville ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Human Lactoferrin

A mWAP–hLF hybrid gene locus gave extremely high level expression of human lactoferrin in the milk of transgenic mice

2009 ◽  
Vol 18 (4) ◽  
pp. 573-582 ◽  
Author(s):  
Gengshou Shi ◽  
Hongxing Chen ◽  
Xiaojie Wu ◽  
Yanrong Zhou ◽  
Zhuguo Liu ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Gene Locus ◽  
Human Lactoferrin ◽  
High Level Expression ◽  
Hybrid Gene ◽  
High Level

High-Level Expression of Human Lactoferrin in Milk of Transgenic Mice Using Genomic Lactoferrin Sequence

1999 ◽  
Vol 126 (2) ◽  
pp. 320-325 ◽  
Author(s):  
S. J. Kim ◽  
B. H. Sohn ◽  
S. Jeong ◽  
K.-W. Pak ◽  
J.-S. Park ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Human Lactoferrin ◽  
High Level Expression ◽  
High Level

Purification and antibacterial activity of recombinant human lactoferrin in milk of transgenic mice

2009 ◽  
Vol 6 (3) ◽  
pp. 253-256
Author(s):  
Miao Ming-Xing ◽  
Yuan Yu-Guo ◽  
An Li-You ◽  
Zhao Jun-Hui ◽  
Bai Ya-Jun ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Transgenic Mice ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Gel Filtration ◽  
Human Lactoferrin ◽  
Diffusion Method ◽  
Enzyme Linked Immunosorbent Assay ◽  
Recombinant Human Lactoferrin ◽  
Agar Disc

AbstractTo verify its antibacterial activity, recombinant human lactoferrin (rhLF) was extracted from the milk of transgenic mice (Mus musculus) (PCL25and AP) by gel filtration chromatography and analysed by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE), Western blot and enzyme-linked immunosorbent assay (ELISA). In addition, its bacteriostatic properties were tested using the agar disc diffusion method. ELISA analysis showed that the concentration of rhLF in the milk of transgenic mice ranged from 7 to 8 mg/ml, and the recombinant protein expressed in the milk had the same molecular weight as the native protein (~78 kDa), indicating that the rhLFs had a strong antibacterial activity onEscherichia coliandSalmonella.

The state of health and the reproductive potential of transgenic mice secreting recombinant human lactoferrin in milk

2009 ◽  
Vol 427 (1) ◽  
pp. 195-198 ◽  
Author(s):  
A. V. Deykin ◽  
T. G. Ermolkevich ◽  
Ya. G. Gursky ◽  
A. N. Krasnov ◽  
S. G. Georgieva ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Reproductive Potential ◽  
The State ◽  
Human Lactoferrin ◽  
State Of Health ◽  
Recombinant Human Lactoferrin

Expression of human lactoferrin in milk of transgenic mice

1994 ◽  
Vol 3 (2) ◽  
pp. 99-108 ◽  
Author(s):  
Gerard J. Platenburg ◽  
Erika P. A. Kootwijk ◽  
Patricia M. Kooiman ◽  
Shelley L. Woloshuk ◽  
Jan H. Nuijens ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Human Lactoferrin

A study of recombinant human lactoferrin secreted in milk of transgenic mice

2006 ◽  
Vol 411 (1) ◽  
pp. 336-338 ◽  
Author(s):  
A. V. Sokolov ◽  
M. O. Pulina ◽  
A. V. Kristiyan ◽  
E. T. Zakharova ◽  
O. L. Runova ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Human Lactoferrin ◽  
Recombinant Human Lactoferrin

scholarly journals Characterization of Recombinant Human Lactoferrin Secreted in Milk of Transgenic Mice

1997 ◽  
Vol 272 (13) ◽  
pp. 8802-8807 ◽  
Author(s):  
Jan H. Nuijens ◽  
Patrick H. C. van Berkel ◽  
Marlieke E. J. Geerts ◽  
Peter Paul Hartevelt ◽  
Herman A. de Boer ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Human Lactoferrin ◽  
Recombinant Human Lactoferrin

scholarly journals How to Obtain a Mega-Intestine with Normal Morphology: In Silico Modelling of Postnatal Intestinal Growth in a Cd97-Transgenic Mouse

2021 ◽  
Vol 22 (14) ◽  
pp. 7345
Author(s):  
Felix Hofmann ◽  
Torsten Thalheim ◽  
Karen Rother ◽  
Marianne Quaas ◽  
Christiane Kerner ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Intestinal Epithelium ◽  
Tissue Growth ◽  
Intestinal Morphology ◽  
Intestinal Cell ◽  
Epithelial Tissue ◽  
Intestinal Growth ◽  
Crypt Fission ◽  
Growth Activation ◽  
Microscopic Morphology

Intestinal cylindrical growth peaks in mice a few weeks after birth, simultaneously with crypt fission activity. It nearly stops after weaning and cannot be reactivated later. Transgenic mice expressing Cd97/Adgre5 in the intestinal epithelium develop a mega-intestine with normal microscopic morphology in adult mice. Here, we demonstrate premature intestinal differentiation in Cd97/Adgre5 transgenic mice at both the cellular and molecular levels until postnatal day 14. Subsequently, the growth of the intestinal epithelium becomes activated and its maturation suppressed. These changes are paralleled by postnatal regulation of growth factors and by an increased expression of secretory cell markers, suggesting growth activation of non-epithelial tissue layers as the origin of enforced tissue growth. To understand postnatal intestinal growth mechanistically, we study epithelial fate decisions during this period with the use of a 3D individual cell-based computer model. In the model, the expansion of the intestinal stem cell (SC) population, a prerequisite for crypt fission, is largely independent of the tissue growth rate and is therefore not spontaneously adaptive. Accordingly, the model suggests that, besides the growth activation of non-epithelial tissue layers, the formation of a mega-intestine requires a released growth control in the epithelium, enabling accelerated SC expansion. The similar intestinal morphology in Cd97/Adgre5 transgenic and wild type mice indicates a synchronization of tissue growth and SC expansion, likely by a crypt density-controlled contact inhibition of growth of intestinal SC proliferation. The formation of a mega-intestine with normal microscopic morphology turns out to originate in changes of autonomous and conditional specification of the intestinal cell fate induced by the activation of Cd97/Adgre5.

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