Transcription and mRNA splicing of the human lactoferrin gene controlled by the regulatory region of the bovine ? S1 casein gene in the mammary gland of transgenic mice and in mouse embryonic stem cells

2005 ◽  
Vol 41 (3) ◽  
pp. 227-232 ◽  
Author(s):  
E. S. Zakharova ◽  
M. V. Pryzhkova ◽  
A. V. Kibardin ◽  
T. G. Ermolkevich ◽  
S. G. Kadulin ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mammary Gland ◽  
Embryonic Stem Cells ◽  
Transgenic Mice ◽  
Regulatory Region ◽  
Embryonic Stem ◽  
Mrna Splicing ◽  
Human Lactoferrin ◽  
Casein Gene ◽  
Lactoferrin Gene

The Use of Genetically Modified Embryonic Stem Cells to Generate Transgenic Mice

2004 ◽  
Vol 40 (3) ◽  
pp. 235-238 ◽  
Author(s):  
M. V. Pryzhkova ◽  
I. R. Zakeeva ◽  
A. V. Kibardin ◽  
G. P. Georgiev ◽  
S. L. Kiselev
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Transgenic Mice ◽  
Genetically Modified ◽  
Embryonic Stem

124 Functional analysis of porcine OCT4 transcriptional regulatory region-based reporter system

2019 ◽  
Vol 31 (1) ◽  
pp. 187
Author(s):  
S.-H. Kim ◽  
K.-H. Choi ◽  
D.-K. Lee ◽  
M. Lee ◽  
M.-H. Cho ◽  
...  
Keyword(s):  
Stem Cells ◽  
Functional Analysis ◽  
Embryonic Stem Cells ◽  
Promoter Region ◽  
Fluorescent Protein ◽  
Regulatory Region ◽  
Embryonic Stem ◽  
Upstream Region ◽  
Reporter Assay ◽  
Reporter System

Gene OCT4 plays pivotal roles in maintaining pluripotency of early mammalian embryonic development and embryonic stem cells. It is essential to establish a reporter system based on the OCT4 promoter region for the study of pluripotency. However, there is still a lack of sufficient information about the porcine OCT4 upstream reporter system. To improve our understanding of the porcine OCT4 regulatory region, first, we conducted an investigation to find conserved regions in the porcine OCT4 promoter upstream region by sequence-based comparative analysis using various mammalian genome sequences. A similarity of nucleotide sequences of the 5′ upstream region was low among mammalian species. However, the OCT4 promoter and 4 regulatory regions including distal and proximal enhancer elements have a high similarity. Next, a functional analysis of the porcine OCT4 promoter region was conducted. Luciferase reporter assay indicated that the porcine OCT4 distal enhancer and proximal enhancer are highly activated in mouse embryonic stem cells and embryonic carcinoma cells, respectively (n=3). Comparison analysis of naïve (Tbx3, Nr0b1, Rex1, Esrrb, Nanog, Klf2) or primed (Gata6, Mixl1, Fgf5, Otx2) state marker gene expression in a dual-reporter assay using pOCT4-DE-eGFP and pOCT4-PE-DsRed2 showed that expression of naïve and primed markers were up-regulated in cells with high green fluorescent protein and red fluorescent protein expression, respectively (n=3). Porcine OCT4-upstream region-based reporter constructs showed exclusive expression patterns depending on the state of pluripotency. This work could provide basic information for the porcine OCT4 upstream region and the various porcine OCT4-fluorescence reporter constructs, which can be applied to study species-specific pluripotency in early embryo development and for the establishment of embryonic stem cells in pigs. This work was supported by the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET) through the Development of High Value-Added Food Technology Program, funded by the Ministry of Agriculture, Food and Rural Affairs (MAFRA, 118042-03-1-HD020).

scholarly journals Targeting of De Novo DNA Methylation Throughout the Oct-4 Gene Regulatory Region in Differentiating Embryonic Stem Cells

PLoS ONE ◽  
2010 ◽  
Vol 5 (4) ◽  
pp. e9937 ◽  
Author(s):  
Rodoniki Athanasiadou ◽  
Dina de Sousa ◽  
Kevin Myant ◽  
Cara Merusi ◽  
Irina Stancheva ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
De Novo ◽  
Regulatory Region ◽  
Embryonic Stem ◽  
Gene Regulatory

Androgenetic haploid embryonic stem cells produce live transgenic mice

Nature ◽  
2012 ◽  
Vol 490 (7420) ◽  
pp. 407-411 ◽  
Author(s):  
Wei Li ◽  
Ling Shuai ◽  
Haifeng Wan ◽  
Mingzhu Dong ◽  
Meng Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Transgenic Mice ◽  
Embryonic Stem ◽  
Androgenetic Haploid

scholarly journals  -Synuclein Alters Notch-1 Expression and Neurogenesis in Mouse Embryonic Stem Cells and in the Hippocampus of Transgenic Mice

2008 ◽  
Vol 28 (16) ◽  
pp. 4250-4260 ◽  
Author(s):  
L. Crews ◽  
H. Mizuno ◽  
P. Desplats ◽  
E. Rockenstein ◽  
A. Adame ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Transgenic Mice ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Notch 1

Rapid selection of XO embryonic stem cells using Y chromosome-linked GFP transgenic mice

2014 ◽  
Vol 23 (5) ◽  
pp. 757-765 ◽  
Author(s):  
Satoshi Yamamoto ◽  
Yasumitsu Nagao ◽  
Kenji Kuroiwa ◽  
Yoji Hakamata ◽  
Masaru Ichida ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Transgenic Mice ◽  
Y Chromosome ◽  
Embryonic Stem ◽  
Selection Of

scholarly journals Identification and Characterization of the OCT4 Upstream Regulatory Region in Sus scrofa

2019 ◽  
Vol 2019 ◽  
pp. 1-11
Author(s):  
Seung-Hun Kim ◽  
Kwang-Hwan Choi ◽  
Dong-Kyung Lee ◽  
Mingyun Lee ◽  
Jae Yeon Hwang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Promoter Region ◽  
Regulatory Region ◽  
Embryonic Stem ◽  
Luciferase Reporter ◽  
Upstream Region ◽  
Fluorescence Signal ◽  
Reporter Assay ◽  
Reporter System

OCT4 plays pivotal roles in maintaining pluripotency during early mammalian embryonic development and in embryonic stem cells. It is essential to establish a reporter system based on the OCT4 promoter region to study pluripotency. However, there is still a lack of information about the porcine OCT4 upstream reporter system. To improve our understanding of the porcine OCT4 regulatory region, we identified conserved regions in the porcine OCT4 promoter upstream region by sequence-based comparative analysis using various mammalian genome sequences. The similarity of nucleotide sequences in the 5′ upstream region was low among mammalian species. However, the OCT4 promoter and four regulatory regions, including distal and proximal enhancer elements, had high similarity. Next, a functional analysis of the porcine OCT4 promoter region was conducted. Luciferase reporter assay results indicated that the porcine OCT4 distal enhancer and proximal enhancer were highly activated in mouse embryonic stem cells and embryonic carcinoma cells, respectively. A comparison analysis of naïve and primed state marker gene expression in a dual-reporter assay showed that the expression levels of naïve and primed markers differed in fluorescence signal between high-expressing cells and low-expressing cells. Similar to OCT4 upstream-based reporter systems derived from other species, the porcine OCT4 upstream region-based reporter constructs showed exclusive expression patterns depending on the state of pluripotency. This work provides basic information about the porcine OCT4 upstream region and various porcine OCT4 fluorescence reporter constructs, which can be applied to study species-specific pluripotency in early embryo development and the establishment of embryonic stem cells in pigs.

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