Vasopressin Gene Expression in Rat Choroid Plexus

1998 ◽  
pp. 59-65 ◽  
Author(s):  
Adam Chodobski ◽  
Brian E. Wojcik ◽  
Y. Peng Loh ◽  
Kimberly A. Dodd ◽  
Joanna Szmydynger-Chodobska ◽  
...  
Keyword(s):  
Gene Expression ◽  
Choroid Plexus ◽  
Vasopressin Gene

scholarly journals Assessment of common housekeeping genes as reference for gene expression studies using RT-qPCR in mouse choroid plexus

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Kim Hoa Ho ◽  
Annarita Patrizi
Keyword(s):  
Gene Expression ◽  
Choroid Plexus ◽  
Sensory Deprivation ◽  
Housekeeping Genes ◽  
Housekeeping Gene ◽  
Experimental Conditions ◽  
Brain Repair ◽  
Expression Studies ◽  
Testing Algorithms ◽  
Gene Expression Studies

AbstractChoroid plexus (ChP), a vascularized secretory epithelium located in all brain ventricles, plays critical roles in development, homeostasis and brain repair. Reverse transcription quantitative real-time PCR (RT-qPCR) is a popular and useful technique for measuring gene expression changes and also widely used in ChP studies. However, the reliability of RT-qPCR data is strongly dependent on the choice of reference genes, which are supposed to be stable across all samples. In this study, we validated the expression of 12 well established housekeeping genes in ChP in 2 independent experimental paradigms by using popular stability testing algorithms: BestKeeper, DeltaCq, geNorm and NormFinder. Rer1 and Rpl13a were identified as the most stable genes throughout mouse ChP development, while Hprt1 and Rpl27 were the most stable genes across conditions in a mouse sensory deprivation experiment. In addition, Rpl13a, Rpl27 and Tbp were mutually among the top five most stable genes in both experiments. Normalisation of Ttr and Otx2 expression levels using different housekeeping gene combinations demonstrated the profound effect of reference gene choice on target gene expression. Our study emphasized the importance of validating and selecting stable housekeeping genes under specific experimental conditions.

Oxytocin and Vasopressin Gene Expression in the Hypothalamo-Neurohypophyseal System of the Rat During the Estrous Cycle, Pregnancy, and Lactation

Endocrinology ◽  
1988 ◽  
Vol 122 (3) ◽  
pp. 945-951 ◽  
Author(s):  
HUBERT H. M. VAN TOL ◽  
ERIC L. M. BOLWERK ◽  
BIN LlU ◽  
J. PETER H. BURBACH
Keyword(s):  
Gene Expression ◽  
Estrous Cycle ◽  
Pregnancy And Lactation ◽  
Vasopressin Gene

The hem of the embryonic cerebral cortex is defined by the expression of multiple Wnt genes and is compromised in Gli3-deficient mice

Development ◽  
1998 ◽  
Vol 125 (12) ◽  
pp. 2315-2325 ◽  
Author(s):  
E.A. Grove ◽  
S. Tole ◽  
J. Limon ◽  
L. Yip ◽  
C.W. Ragsdale
Keyword(s):  
Gene Expression ◽  
Cerebral Cortex ◽  
Choroid Plexus ◽  
Gene Families ◽  
Cubitus Interruptus ◽  
Embryonic Mouse ◽  
Developmental Relationship ◽  
Cortical Hem ◽  
Deficient Mice

In the developing vertebrate CNS, members of the Wnt gene family are characteristically expressed at signaling centers that pattern adjacent parts of the neural tube. To identify candidate signaling centers in the telencephalon, we isolated Wnt gene fragments from cDNA derived from embryonic mouse telencephalon. In situ hybridization experiments demonstrate that one of the isolated Wnt genes, Wnt7a, is broadly expressed in the embryonic telencephalon. By contrast, three others, Wnt3a, 5a and a novel mouse Wnt gene, Wnt2b, are expressed only at the medial edge of the telencephalon, defining the hem of the cerebral cortex. The Wnt-rich cortical hem is a transient, neuron-containing, neuroepithelial structure that forms a boundary between the hippocampus and the telencephalic choroid plexus epithelium (CPe) throughout their embryonic development. Indicating a close developmental relationship between the cortical hem and the CPe, Wnt gene expression is upregulated in the cortical hem both before and just as the CPe begins to form, and persists until birth. In addition, although the cortical hem does not show features of differentiated CPe, such as expression of transthyretin mRNA, the CPe and cortical hem are linked by shared expression of members of the Bmp and Msx gene families. In the extra-toesJ (XtJ) mouse mutant, telencephalic CPe fails to develop. We show that Wnt gene expression is deficient at the cortical hem in XtJ/XtJ mice, but that the expression of other telencephalic developmental control genes, including Wnt7a, is maintained. The XtJ mutant carries a deletion in Gli3, a vertebrate homolog of the Drosophila gene cubitus interruptus (ci), which encodes a transcriptional regulator of the Drosophila Wnt gene, wingless. Our observations indicate that Gli3 participates in Wnt gene regulation in the vertebrate telencephalon, and suggest that the loss of telencephalic choroid plexus in XtJ mice is due to defects in the cortical hem that include Wnt gene misregulation.

scholarly journals Regulation of Vasopressin Gene Expression by cAMP and Glucocorticoids in Parvocellular Neurons of the Paraventricular Nucleus in Rat Hypothalamic Organotypic Cultures

2003 ◽  
Vol 23 (32) ◽  
pp. 10231-10237 ◽  
Author(s):  
Shinobu Kuwahara ◽  
Hiroshi Arima ◽  
Ryouichi Banno ◽  
Ikuko Sato ◽  
Noriko Kondo ◽  
...  
Keyword(s):  
Gene Expression ◽  
Paraventricular Nucleus ◽  
Organotypic Cultures ◽  
Parvocellular Neurons ◽  
Vasopressin Gene

scholarly journals Altered choroid plexus gene expression in major depressive disorder

2014 ◽  
Vol 8 ◽  
Author(s):  
Cortney A. Turner ◽  
Robert C. Thompson ◽  
William E. Bunney ◽  
Alan F. Schatzberg ◽  
Jack D. Barchas ◽  
...  
Keyword(s):  
Gene Expression ◽  
Major Depressive Disorder ◽  
Depressive Disorder ◽  
Choroid Plexus ◽  
Major Depressive

Oxytocin and vasopressin gene expression during gestation and lactation

1988 ◽  
Vol 4 (1) ◽  
pp. 1-6 ◽  
Author(s):  
Hans H. Zingg ◽  
Diana L. Lefebvre
Keyword(s):  
Gene Expression ◽  
Vasopressin Gene

LEPTIN RECEPTOR GENE EXPRESSION IN THE CHOROID PLEXUS IS INFLUENCED BY STAGE OF DEVELOPMENT AND MATERNAL STRESS IN THE GILT

2007 ◽  
Vol 77 (Suppl_1) ◽  
pp. 171-171
Author(s):  
Chad O'Gorman ◽  
Elizabeth Gonzales ◽  
Matthew Eaton ◽  
Paige Williams ◽  
Maribel Reyna ◽  
...  
Keyword(s):  
Gene Expression ◽  
Choroid Plexus ◽  
Leptin Receptor ◽  
Maternal Stress ◽  
Receptor Gene ◽  
Stage Of Development ◽  
Receptor Gene Expression
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