Immunohistochemical analysis of the adaptation of adult guinea-pig cardiomyocytes in long-term cultures and in cocultures with cardiac neurons: A novel model for studies of myocardial function

The purpose of this article is to offer an improved understanding of how parents of children with long-term disabilities are empowered to successfully take up their role as decision-making partners in the design and delivery of the care of their child. The intention is to stimulate dialogue, encourage reflection and provide practical suggestions for health professionals working with children and their families. The reported findings are from a study which was guided by a constructivist grounded theory methodology. This involved an iterative process of repeated cycles of data collection and analysis, which comprised 12 semi-structured, in-depth interviews with 14 parents of children accessing paediatric services within a single National Health Service Trust. A novel model, explaining how the power im/balance and the perceived state of the therapeutic relationship influence how successfully a parent takes up their position in the collaborative partnership, is presented and discussed. It is suggested that by thoughtfully addressing the traditional hierarchy that exists within healthcare, health professionals might facilitate the development of a ‘truly’ therapeutic relationship, which can help promote parental empowerment.

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Primary Intrapulmonary Thymomas: Case Report and Review of the Literature

Journal of International Medical Research ◽

10.1177/147323000903700434 ◽

2009 ◽

Vol 37 (4) ◽

pp. 1252-1257 ◽

Cited By ~ 8

Author(s):

L Gong ◽

Y-H Li ◽

X-L He ◽

Q Wang ◽

L Yao ◽

...

Keyword(s):

Epithelial Membrane Antigen ◽

Immunohistochemical Analysis ◽

Lung Tumour ◽

Review Of The Literature ◽

Malignant Tumours ◽

Pathological Characteristics ◽

Immunological Phenotype ◽

Type A Thymoma

Primary intrapulmonary thymomas (PIT), which are intrapulmonary tumours without an associated mediastinal component, are very rare; only 29 cases of PIT have been described in the literature since 1951. This report presents a case of PIT in a 59-year old Chinese woman with a type A thymoma (including its pathogenesis, clinical pathological characteristics, immunological phenotype, treatment and prognosis), in the context of a review of the current literature. The origin of thymomas in this unusual location remains unknown. In this case, immunohistochemical analysis demonstrated that the epithelial component was strongly positive for cytokeratin and focally reactive for epithelial membrane antigen. It is concluded that PIT should be considered when the histopathological appearance of a lung tumour is not typical of other pulmonary neoplasms. Complete resection appears sufficient in non-malignant tumours, while in cases of partial resection or malignancy, adjuvant radiotherapy should be considered. Long-term regular clinical follow-up is also warranted, due to the risk of late local recurrence.

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THE EFFECT OF LONG TERM TREATMENT WITH A ?-ADRENOCEPTOR BLOCKER, l-PROPRANOLOL, ON THE DISTRIBUTION OF CARDIAC OUTPUT OF THE LATE GESTATIONAL GUINEA-PIG

Clinical and Experimental Pharmacology and Physiology ◽

10.1111/j.1440-1681.1983.tb00173.x ◽

1983 ◽

Vol 10 (1) ◽

pp. 71-75 ◽

Cited By ~ 2

Author(s):

Leif Bjellin ◽

Lena Mårtensson

Keyword(s):

Cardiac Output ◽

Guinea Pig ◽

Term Treatment ◽

Long Term Treatment ◽

Adrenoceptor Blocker ◽

Distribution Of Cardiac Output

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Isolation and long term preservation of pancreatic islets from mouse, rat and guinea pig

Diabetologia ◽

10.1007/bf00428975 ◽

1976 ◽

Vol 12 (2) ◽

pp. 115-121 ◽

Cited By ~ 29

Author(s):

J. Ferguson ◽

R. H. Allsopp ◽

R. M. R. Taylor ◽

I. D. A. Johnston

Keyword(s):

Guinea Pig ◽

Pancreatic Islets ◽

Long Term Preservation

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Formation of long-term potentiation in superior colliculus slices from the guinea pig

Neuroscience Letters ◽

10.1016/0304-3940(89)90251-6 ◽

1989 ◽

Vol 96 (1) ◽

pp. 108-113 ◽

Cited By ~ 101

Author(s):

Yasuhiro Okada ◽

Takaaki Miyamoto

Keyword(s):

Guinea Pig ◽

Superior Colliculus ◽

Long Term Potentiation ◽

Term Potentiation

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High environmental salinity induces memory enhancement and increases levels of brain angiotensin-like peptides in the crab Chasmagnathus granulatus

Journal of Experimental Biology ◽

10.1242/jeb.203.22.3369 ◽

2000 ◽

Vol 203 (22) ◽

pp. 3369-3379 ◽

Cited By ~ 1

Author(s):

A. Delorenzi ◽

B. Dimant ◽

L. Frenkel ◽

V.E. Nahmod ◽

D.R. Nassel ◽

...

Keyword(s):

Angiotensin Ii ◽

Brackish Water ◽

Sea Water ◽

Immunohistochemical Analysis ◽

Facilitatory Effect ◽

Long Term Memory ◽

Stimulus Context ◽

Memory Enhancement ◽

Chasmagnathus Granulatus

Previous work on the brackish-water crab Chasmagnathus granulatus demonstrated that an endogenous peptide similar to angiotensin II plays a significant role in enhancing long-term memory that involves an association between context and an iterative danger stimulus (context-signal memory). The present results show that this memory enhancement could be produced by moving crabs from brackish water to sea water (33.0%) and keeping them there for at least 4 days. The possibility that such a facilitatory effect is due to osmotic stress is ruled out. Coincidentally, the level of angiotensin-II-like peptides in crab brain, measured by radioimmunoassay, increases with the length of exposure to sea water, reaching a significantly different level at the fourth day. The presence of angiotensin-II-like immunoreactive material in neural structures of the supraoesophageal and eyestalk ganglia was confirmed by immunohistochemical analysis. The results are interpreted as supporting the hypothesis that exposure to water of high salinity is an external cue triggering a process mediated by angiotensins that leads to enhanced memory in these crabs.

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Abstract P195: No Functional Nerve Recovery To 6 Months Post Renal Denervation With Rf Energy In A Normotensive Pig Model

Hypertension ◽

10.1161/hyp.78.suppl_1.p195 ◽

2021 ◽

Vol 78 (Suppl_1) ◽

Author(s):

Andrew Sharp ◽

Stefan Tunev ◽

Markus P Schlaich ◽

David P LEE ◽

Aloke Finn ◽

...

Keyword(s):

Animal Studies ◽

Renal Denervation ◽

Blood Pressure Reduction ◽

Immunohistochemical Analysis ◽

Swine Model ◽

Renal Nerve ◽

Tissue Samples ◽

Axon Density ◽

Rf Energy

Background: The safety and efficacy of catheter-based radio frequency (RF) renal denervation (RDN) have been demonstrated in randomized, sham-controlled trials. Long-term durability of blood pressure reduction following RDN has also been demonstrated by all-comer registries, although published pre-clinical reports of functional renal nerve regrowth are not consistent. We quantified the processes that support RDN procedural durability utilizing animal models. Methods: Animal studies were conducted in accordance with published guidelines. RDN was performed (4 lesions in the main renal artery) in normotensive swine using the Symplicity Spyral™ RDN system (Medtronic, Santa Rosa, CA, USA). Two additional groups not undergoing RDN served as control. Serial histological tissue samples were obtained in separate groups at 7 (n=12/group) and 180 (N=16/group) days post-procedure in all animals followed by bioanalytical quantification of cortical norepinephrine (NE) levels and immunohistochemical analysis of renal cortical axon density in matched samples. Results: Renal cortical axon density and NE levels were significantly reduced at 7 days and persisted through 180 days following RDN compared with control ( Figure ). Nerve fibrosis and necrosis were observed in the region of ablation, while nerve body atrophy was apparent distal to ablation location at 180 days. Conclusions: Reductions in both NE and renal cortical axon density were sustained at 7 and 180 days post-RDN procedure using RF renal denervation in a normotensive swine model. These data confirm and extend other pre-clinical and clinical evidence of long-term durability of the RDN procedure using RF energy.

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