Light Scattering Analysis of Single Cells

Cell Analysis ◽  
1982 ◽  
pp. 111-143 ◽  
Author(s):  
Gary C. Salzman
Keyword(s):  
Light Scattering ◽  
Single Cells

scholarly journals 2D light scattering patterns of mitochondria in single cells

2007 ◽  
Vol 15 (17) ◽  
pp. 10562 ◽  
Author(s):  
Xuan-Tao Su ◽  
Clarence Capjack ◽  
Wojciech Rozmus ◽  
Christopher Backhouse
Keyword(s):  
Light Scattering ◽  
Single Cells

scholarly journals Elastic Light Scattering from Single Cells: Orientational Dynamics in Optical Trap

2004 ◽  
Vol 87 (2) ◽  
pp. 1298-1306 ◽  
Author(s):  
Dakota Watson ◽  
Norbert Hagen ◽  
Jonathan Diver ◽  
Philippe Marchand ◽  
Mirianas Chachisvilis
Keyword(s):  
Light Scattering ◽  
Single Cells ◽  
Optical Trap ◽  
Elastic Light Scattering ◽  
Orientational Dynamics

A Twelve-Channel Automatic Device for Continuous Recording of Cell Aggregation by Measurement of Small-Angle Light-Scattering

1980 ◽  
Vol 41 (1) ◽  
pp. 1-18
Author(s):  
WILLIAM A. THOMAS ◽  
MALCOLM S. STEINBERG
Keyword(s):  
Light Scattering ◽  
Single Cells ◽  
Cell Aggregation ◽  
Automatic Device ◽  
Great Sensitivity ◽  
Photomultiplier Tube ◽  
Steel Blank ◽  
Silicone Grease ◽  
Beam Stop ◽  
A Cell

We describe here a 12-channel aggregometer, evolved from the instrument described by Beug & Gerisch in 1972, which records the course of aggregation or agglutination of cells or other particles by following the changes in light-scattering of the aggregating suspension. The instrument incorporates a simple memory system for condensing the data, introduces an improved cuvette design, and can be adjusted in its responsiveness to light-scattering by particles of different sizes. In our aggregometer, a vertical wheel in an opaque thermostatted chamber accommodates up to 12 cuvettes each containing a cell suspension and an air bubble. The constant rising of the bubble as the wheel rotates causes the stirring action which promotes aggregation. At a certain point in its rotational path, each cuvette is penetrated by a beam of light focused upon an absorbing beam stop centred in front of a photomultiplier tube. Particles suspended in the cuvette scatter a portion of the light beyond the beam stop into the photomultiplier tube to produce an electrical pulse proportional to the photon input. Collection of these pulses with the wheel in continuous motion avoids any disturbance of the course of aggregation. The pulses are routed to memory circuits for summation and eventual recording by a 12-channel printing potentiometer which automatically colour-codes and numbers each curve. The cuvettes consist of 2 glass microscope coverslips affixed with silicone grease over a hole in a thin, stainless steel blank. They are very durable and are easily dismantled and reassembled for cleaning. The coverslips are replaced after each use. The discoid chamber of our cuvette permits uniform circulation of the bubble, which in turn causes gentle stirring of the aggregating suspension at a rate that is a direct and continuous function of rev/min. Measurement of light-scattering at small angles (3-5.5°) provides great sensitivity to the disappearance of single cells and progressively less sensitivity to the coalescence of aggregates of progressively larger size. The signal generated by the photomultiplier tube decreases as aggregation proceeds. At 32 rev/min, one data point per channel, representing the sum (average) of 24 successive measurements, is printed every 458. In the course of a 60-min assay under standard conditions, up to 23040 individual readings can be automatically taken, summed and plotted. In a comparison of 8 identical samples run simultaneously, the printed values after 60 min agreed with a standard deviation of ±2%.

Oxygen Nanobubble Tracking by Light Scattering in Single Cells and Tissues

ACS Nano ◽  
2017 ◽  
Vol 11 (3) ◽  
pp. 2682-2688 ◽  
Author(s):  
Pushpak Bhandari ◽  
Xiaolei Wang ◽  
Joseph Irudayaraj
Keyword(s):  
Light Scattering ◽  
Single Cells

3D-printing-based microfluidic device for fast light scattering imaging of single cells

2021 ◽  
Author(s):  
Ting Liu ◽  
Xiaohang Zhou ◽  
Zejie Lin ◽  
Jiale Chen ◽  
Meiai Lin
Keyword(s):  
Light Scattering ◽  
3D Printing ◽  
Microfluidic Device ◽  
Single Cells ◽  
Fast Light

scholarly journals Degranulation, membrane addition, and shape change during chemotactic factor-induced aggregation of human neutrophils.

1982 ◽  
Vol 95 (1) ◽  
pp. 234-241 ◽  
Author(s):  
S T Hoffstein ◽  
R S Friedman ◽  
G Weissmann
Keyword(s):  
Light Scattering ◽  
Cell Surface ◽  
Cell Shape ◽  
Shape Change ◽  
Single Cells ◽  
Chemotactic Factor ◽  
Human Neutrophils ◽  
Transient Change ◽  
Membrane Addition ◽  
Induced Aggregation

Neutrophils stimulated by the chemotactic factor formyl-methionyl-leucyl-phenyl-alanine (FMLP) undergo a transient change in surface properties that permits the cells to adhere more readily to surfaces and to each other. This transient change can be monitored by light scattering as stimulated neutrophils form aggregates while stirred in a platelet aggregometer. Maximum change in light scattering occurs within 1 min and correlates with an increase in the percentage of cells that are in aggregates of four or more cells and a decrease in the percentage of single cells. With time (3-5 min), small aggregates disappear and single cells reappear. The transient change in adhesiveness is accompanied by a persistent change in cell shape; the cells become polarized and protrude ruffles from one sector of the cell surface. During aggregation the cells adhere to one another with smooth sides together and ruffles pointed outward. During disaggregation the cells dissociate laterally with the simultaneous internalization of membrane in the region opposite the ruffles. Particle bound to the surface by charge (thorotrast, cationized ferritin) are concentrated and internalized in this region. The change in cell shape from round to ruffled occurs within seconds, suggesting that membrane is added to the cell surface from an intracellular store. We therefore quantified surface membrane by electron microscopy morphometry and measured a 25% increase within 10 s of adding FMLP. The source of new membrane appeared to be the specific granule membrane since the kinetics of granule discharge (between 30% and 50% of all release occurs in the first 10 s) correlate with the appearance of new membrane. Furthermore, the amount of membrane that appears at the cell surface at 10 s correlates with that lost from intracellular granules in that time. Chemotaxin-induced aggregation thus begins with granule discharge and membrane addition followed by protrusion of ruffles. Adherence is maximal at 60 s and the gradual loss of adhesiveness that follows is associated with uropod formation and enhanced endocytic activity.

Elastic light scattering studies from single cells in optical trap

2006 ◽  
Author(s):  
Dakota Watson ◽  
Norbert Hagen ◽  
Jonathan Diver ◽  
Philippe Marchand ◽  
Mirianas Chachisvilis
Keyword(s):  
Light Scattering ◽  
Single Cells ◽  
Optical Trap ◽  
Elastic Light Scattering

scholarly journals Optoacoustic flow-cytometry with light scattering referencing

2020 ◽  
Author(s):  
Markus Seeger ◽  
Andre C. Stiel ◽  
Vasilis Ntziachristos
Keyword(s):  
Light Scattering ◽  
Fluorescent Proteins ◽  
Single Cells ◽  
Optoacoustic Imaging ◽  
E Coli ◽  
Specificity And Sensitivity ◽  
Ultrasound Backscatter ◽  
Molecular Specificity ◽  
And Function

AbstractIn analogy to the development of fluorescent proteins, innovative tools for screening optoacoustic cell labels could lead to tailored protein labels for OA, imparting novel ways to visualize biological structure and function. Optoacoustic imaging emerges towards a highly promising modality for life sciences and medical practise with advantageous capabilities such as great accessible depth, and 3D studying of living tissue. The development of novel labels with molecular specificity could significantly enhance the optoacoustic contrast, specificity, and sensitivity and allow optoacoustic to interrogate tissues not amenable to the fluorescence method. We report on an optoacoustic flow cytometer (OAFC) prototype, developed for screening optoacoustic reporter genes. The cytometer concurrently records light scattering for referencing purposes. Since recording light scattering is completely independent from OA, we believe it to be a more reliable referencing method than e.g. fluorescence or ultrasound-backscatter. Precise characterization of our OAFC prototype showcases its ability to optoacoustically characterize objects in-flow that are in the size range of single cells. We apply the OAFC to distinguish individual E. coli cells based on optoacoustic properties of their expressed chromoproteins read in-flow using microfluidic arrangements and achieved precisions over 90%. We discuss how the light scattering referenced OAFC method offers a critical step towards routine measurement of optoacoustic properties of single-cells and could pave the way for identifying genetically encoded optoacoustic reporters, by transferring working concepts of the fluorescence field.

scholarly journals Phase-sensitive, angle-resolved light-scattering microscopy of single cells

2020 ◽  
Vol 45 (24) ◽  
pp. 6775
Author(s):  
Robert L. Draham ◽  
Kaitlin J. Dunn ◽  
Andrew J. Berger
Keyword(s):  
Light Scattering ◽  
Single Cells ◽  
Phase Sensitive
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