A Fertility Regulating Vaccine Based on the Carboxyl-Terminal Peptide of the Beta Subunit of Human Chorionic Gonadotrophin

1986 ◽  
pp. 43-59 ◽  
Author(s):  
P. D. Griffin
Keyword(s):  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Beta Subunit ◽  
Carboxyl Terminal

IMPROVED DIAGNOSIS OF PREGNANCY-RELATED GYNAECOLOGICAL EMERGENCIES BY RAPID HUMAN CHORIONIC GONADOTROPHIN BETA-SUBUNIT ASSAY

1981 ◽  
Vol 88 (2) ◽  
pp. 138-140 ◽  
Author(s):  
M. Seppala ◽  
T. Ranta ◽  
E.-M. Rutanen ◽  
U.-H. Stenman ◽  
K. Tontti ◽  
...  
Keyword(s):  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Beta Subunit

scholarly journals High expression of human chorionic gonadotrophin beta-subunit using a synthetic vaccinia virus promoter

2001 ◽  
Vol 26 (3) ◽  
pp. 281-287 ◽  
Author(s):  
A Gupta ◽  
S Chandrasekhar ◽  
R Pal ◽  
S Ahlawat ◽  
O Singh
Keyword(s):  
Vaccinia Virus ◽  
Recombinant Vaccinia Virus ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Beta Subunit ◽  
Synthetic Promoter ◽  
Contraceptive Vaccine ◽  
Virus Rna ◽  
Early Late ◽  
Virus Promoter

We have constructed a recombinant vaccinia virus to express the beta-subunit of human chorionic gonadotrophin (betahCG), a secretory glycoprotein that is used as an antigen for a contraceptive vaccine. The cDNA encoding the subunit was cloned under the control of a synthetic promoter that could be recognised by a vaccinia virus RNA polymerase to direct transcription. The peak expression level of betahCG directed by a late synthetic promoter (Psyn) was 11.5 microg/ml, a level that was at least sixfold higher than that directed by the p7.5 early/late promoter. The expressed protein was correctly processed post-translationally such that it attained a conformation with correctly folded discontinuous epitope(s) similar to that seen in native betahCG.

The molecular basis for epitopes on the free β-subunit of human chorionic gonadotrophin (hCG), its carboxyl-terminal peptide and the hCGβ-core fragment

1994 ◽  
Vol 141 (1) ◽  
pp. 153-162 ◽  
Author(s):  
S Dirnhofer ◽  
S Madersbacher ◽  
J-M Bidart ◽  
P B W Ten Kortenaar ◽  
G Spöttl ◽  
...  
Keyword(s):  
Amino Acid ◽  
Molecular Basis ◽  
Tertiary Structure ◽  
Solid Phase ◽  
Critical Role ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Antigenic Determinants ◽  
Β Subunit ◽  
Carboxyl Terminal

Abstract The molecular basis for antigenic determinants on the free β-subunit of human chorionic gonadotrophin (hCGβ), its carboxyl-terminal peptide (hCGβCTP) and the hCGβcore fragment (hCGβcf) was elucidated by means of monoclonal antibodies (MCAs). The objective of the present study was to resolve the antigenic topography of these three molecules in terms of epitope identification at different levels of structural organization as well as analysis of their spatial arrangement. An hCGβcf preparation, a synthetic peptide corresponding to the hCGβCTP (β109–145), overlapping synthetic peptides spanning the entire amino acid sequence of hCGβ, and a reduced and alkylated hCGβ preparation were assayed in a solid-phase one-site enzyme-linked immunoassay and in a solublephase direct-binding radioimmunoassay (RIA) or competitive RIA. The antigenic topography was mapped by incorporating the MCAs into two-site binding assays. On the surface of free hCGβ, nine different epitopes (β1–β9), arranged in three spatially distinct domains, could be distinguished. Epitopes β1–β7 were located in a single large domain on both hCGβ and the hCGβcf whereas hCGβCTP contained two topographically distant determinants, designated β8 and β9 respectively. All but the two epitopes located on hCGβCTP (β8 and β9) were destroyed by reducing and alkylating hCGβ, suggesting that most antigenic determinants are predominantly non-contiguous and require an intact tertiary structure whereas the molecular structure of hCGβCTP is linear. At a molecular level, amino acid residues spanning hCGβ 45–52, hCGβ 137–144 and hCGβ 113–116 contributed to the formation of epitopes β5, β8 and β9 respectively. We have also shown that the hCGβcf represents the immunodominant part of the free β-subunit of hCG, containing seven mainly conformationally determined epitopes, one of which has a share of the sequence β45–52. The hCGβCTP does not play a critical role in the immunologically important tertiary structure of hCGβ and was itself found to be a predominantly continuous sequence also within the native hormone, expressing two spatially distant antigenic determinants located within residues β113–116 and β137–144 respectively. Journal of Endocrinology (1994) 141, 153–162

scholarly journals Predictive value of plasma human chorionic gonadotrophin beta subunit in diagnosing ectopic pregnancy after in vitro fertilisation and embryo transfer.

BMJ ◽  
1987 ◽  
Vol 294 (6573) ◽  
pp. 667-670 ◽  
Author(s):  
S H Okamoto ◽  
D L Healy ◽  
L M Morrow ◽  
P A Rogers ◽  
A O Trounson ◽  
...  
Keyword(s):  
Ectopic Pregnancy ◽  
Embryo Transfer ◽  
Predictive Value ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Beta Subunit ◽  
In Vitro Fertilisation
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