Storage and Translation of Ferritin Messenger RNA

High performance Nanogold™-in situ hybridzation and its use in the detection of hybridized and PCR-amplified microscopical preparations

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100166944 ◽

1996 ◽

Vol 54 ◽

pp. 896-897

Author(s):

G. W. Hacker ◽

I. Zehbe ◽

J. Hainfeld ◽

A.-H. Graf ◽

C. Hauser-Kronberger ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Messenger Rna ◽

High Performance ◽

Detection System ◽

Direct Methods ◽

Genetic Alterations ◽

Chain Reaction ◽

Protein Encoding ◽

Polymerase Chain

In situ hybridization (ISH) with biotin-labeled probes is increasingly used in histology, histopathology and molecular biology, to detect genetic nucleic acid sequences of interest, such as viruses, genetic alterations and peptide-/protein-encoding messenger RNA (mRNA). In situ polymerase chain reaction (PCR) (PCR in situ hybridization = PISH) and the new in situ self-sustained sequence replication-based amplification (3SR) method even allow the detection of single copies of DNA or RNA in cytological and histological material. However, there is a number of considerable problems with the in situ PCR methods available today: False positives due to mis-priming of DNA breakdown products contained in several types of cells causing non-specific incorporation of label in direct methods, and re-diffusion artefacts of amplicons into previously negative cells have been observed. To avoid these problems, super-sensitive ISH procedures can be used, and it is well known that the sensitivity and outcome of these methods partially depend on the detection system used.

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Changes in protein synthesis and translatable messenger RNA populations associated with ABA induced cold hardiness in potato (Solanum commersonii)

Physiologia Plantarum ◽

10.1034/j.1399-3054.1991.810310.x ◽

1991 ◽

Vol 81 (3) ◽

pp. 349-358 ◽

Cited By ~ 1

Author(s):

M. J. Tseng ◽

P. H. Li

Keyword(s):

Protein Synthesis ◽

Messenger Rna ◽

Cold Hardiness ◽

Solanum Commersonii

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Humane Papillomvirus Messenger RNA aus Lymphknoten zur Sentineldiagnostik bei Patientinnen mit Zervixkarzinom

10.1055/s-0038-1671048 ◽

2018 ◽

Author(s):

M Jentschke ◽

E Bau ◽

R Hass ◽

H Hertel ◽

J Kampers ◽

...

Keyword(s):

Messenger Rna

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DEGRADATION OF MESSENGER RNA IN MAMMALIAN CELLS

Acta Endocrinologica ◽

10.1530/acta.0.071s369 ◽

1972 ◽

Vol 71 (2_Suppla) ◽

pp. S369-S380 ◽

Cited By ~ 7

Author(s):

Francis T. Kenney ◽

Kai-Lin Lee ◽

Charles D. Stiles

Keyword(s):

Messenger Rna ◽

Mammalian Cells ◽

Messenger Rnas ◽

Tyrosine Transaminase

ABSTRACT Analyses of the response of hydrocortisone-induced tyrosine transaminase in cultured H-35 cells to inhibitors of translation (cycloheximide, puromycin) suggest: (1) that bound ribosomes stabilize messenger RNA in vivo; (2) that messenger is degraded at a rate determined by the rate of translation. Since specific messenger RNAs of mammalian cells are degraded at quite different rates, there may be extensive heterogeneity either in the rate at which ribosomes traverse different messengers or in the number of ribosomes which translate specific messenger RNAs.

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