The Lentivirus Regulatory Proteins REV and REX are Site Specific RNA Binding Proteins

1991 ◽  
pp. 15-22 ◽  
Author(s):  
G. King Farrington ◽  
Paul Lynch ◽  
Amy Jensen ◽  
Ernst Böhnlein ◽  
Reed Doten ◽  
...  
Keyword(s):  
Binding Proteins ◽  
Rna Binding ◽  
Rna Binding Proteins ◽  
Regulatory Proteins ◽  
Site Specific

scholarly journals Can we observe changes in mRNA “state”? Overview of methods to study mRNA interactions with regulatory proteins relevant in cancer related processes

The Analyst ◽  
2016 ◽  
Vol 141 (2) ◽  
pp. 548-562 ◽  
Author(s):  
C. Zurla ◽  
J. Jung ◽  
P. J. Santangelo
Keyword(s):  
Binding Proteins ◽  
Rna Binding ◽  
Rna Binding Proteins ◽  
Regulatory Proteins ◽  
Ribonucleic Acids

RNA binding proteins (RBP) regulate the editing, localization, stabilization, translation, and degradation of ribonucleic acids (RNA) through their interactions with specificcis-acting elements within target RNAs.

scholarly journals Unbiased identification of trans regulators of ADAR and A-to-I RNA editing

2019 ◽  
Author(s):  
Emily C. Freund ◽  
Anne L. Sapiro ◽  
Qin Li ◽  
Sandra Linder ◽  
James J. Moresco ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Rna Editing ◽  
Binding Proteins ◽  
Rna Binding ◽  
Rna Binding Proteins ◽  
Neuroblastoma Cells ◽  
Negative Regulator ◽  
Interacting Proteins ◽  
The Novel ◽  
Site Specific

AbstractAdenosine-to-Inosine RNA editing is catalyzed by ADAR enzymes that deaminate adenosine to inosine. While many RNA editing sites are known, few trans regulators have been identified. We perform BioID followed by mass-spectrometry to identify trans regulators of ADAR1 and ADAR2 in HeLa and M17 neuroblastoma cells. We identify known and novel ADAR-interacting proteins. Using ENCODE data we validate and characterize a subset of the novel interactors as global or site-specific RNA editing regulators. Our set of novel trans regulators includes all four members of the DZF-domain-containing family of proteins: ILF3, ILF2, STRBP, and ZFR. We show that these proteins interact with each ADAR and modulate RNA editing levels. We find ILF3 is a global negative regulator of editing. This work demonstrates the broad roles RNA binding proteins play in regulating editing levels and establishes DZF-domain containing proteins as a group of highly influential RNA editing regulators.

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