High-Content Screening: Understanding and Managing Mechanistic Data to Better Predict Toxicity

2015 ◽  
pp. 317-344
Author(s):  
Paul Walker ◽  
Tim Smith ◽  
Kerry Frost ◽  
Sarah Kelly ◽  
Ignacio Gonzalez Suarez
Keyword(s):  
High Content Screening

High-content screening in infectious diseases

2011 ◽  
Vol 15 (4) ◽  
pp. 534-539 ◽  
Author(s):  
Priscille Brodin ◽  
Thierry Christophe
Keyword(s):  
Infectious Diseases ◽  
High Content Screening

High-content screening of drug-induced cardiotoxicity using quantitative single cell imaging cytometry on microfluidic device

Lab on a Chip ◽  
2011 ◽  
Vol 11 (1) ◽  
pp. 104-114 ◽  
Author(s):  
Min Jung Kim ◽  
Su Chul Lee ◽  
Sukdeb Pal ◽  
Eunyoung Han ◽  
Joon Myong Song
Keyword(s):  
Single Cell ◽  
Microfluidic Device ◽  
Cell Imaging ◽  
High Content Screening ◽  
Drug Induced ◽  
Single Cell Imaging ◽  
Imaging Cytometry

scholarly journals The application of CRISPR technology to high content screening in primary neurons

2017 ◽  
Vol 80 ◽  
pp. 170-179 ◽  
Author(s):  
Ben L. Callif ◽  
Brian Maunze ◽  
Nick L. Krueger ◽  
Matthew T. Simpson ◽  
Murray G. Blackmore
Keyword(s):  
High Content Screening ◽  
Primary Neurons

High content screening: seeing is believing

2010 ◽  
Vol 28 (5) ◽  
pp. 237-245 ◽  
Author(s):  
Fabian Zanella ◽  
James B. Lorens ◽  
Wolfgang Link
Keyword(s):  
High Content Screening

scholarly journals A Novel Cell-Based, High-Content Assay for Phosphorylation of Lats2 by Aurora A

2011 ◽  
Vol 16 (8) ◽  
pp. 925-931 ◽  
Author(s):  
Amy Emery ◽  
David A. Sorrell ◽  
Stacy Lawrence ◽  
Emma Easthope ◽  
Mark Stockdale ◽  
...  
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
Kinase Activity ◽  
High Content Screening ◽  
Aurora A Kinase ◽  
Aurora A ◽  
Assay Performance ◽  
A Cell ◽  
Significant Addition ◽  
Specific Inhibitors

Aurora A kinase is a key regulator of mitosis, which is upregulated in several human cancers, making it a potential target for anticancer therapeutics. Consequently, robust medium- to high-throughput cell-based assays to measure Aurora A kinase activity are critical for the development of small-molecule inhibitors. Here the authors compare measurement of the phosphorylation of two Aurora A substrates previously used in high-content screening Aurora A assays, Aurora A itself and TACC3, with a novel substrate Lats2. Using antibodies directed against phosphorylated forms of Aurora A (pThr288), P-TACC3 (pSer558), and P-Lats2 (pSer83), the authors investigate their suitability in parallel for development of a cell-based assay using several reference Aurora inhibitors: MLN8054, VX680, and AZD1152-HQPA. They validate a combined assay of target-specific phosphorylation of Lats2 at the centrosome and an increase in mitotic index as a measure of Aurora A activity. The assay is both sensitive and robust and has acceptable assay performance for high-throughput screening or potency estimation from concentration–response assays. It has the advantage that it can be carried out using a commercially available monoclonal antibody against phospho-Lats2 and the widely available Cellomics ArrayScan HCS reader and thus represents a significant addition to the tools available for the identification of Aurora A specific inhibitors.

scholarly journals Cell-Based Assay Design for High-Content Screening of Drug Candidates

2016 ◽  
Vol 26 (2) ◽  
pp. 213-225 ◽  
Author(s):  
Gregory Nierode ◽  
Paul S. Kwon ◽  
Jonathan S. Dordick ◽  
Seok-Joon Kwon
Keyword(s):  
High Content Screening ◽  
Drug Candidates ◽  
Assay Design

High Throughput and High Content Screening Using Peptides

2005 ◽  
pp. 347-384
Author(s):  
Paul Hamilton ◽  
Robert Carlson ◽  
Robin Hyde-Deruyscher
Keyword(s):  
High Throughput ◽  
High Content Screening
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