Skeletal Muscle Culture Under Spaceflight Conditions

2016 ◽  
pp. 151-174
Author(s):  
Mark S. F. Clarke
Keyword(s):  
Skeletal Muscle ◽  
Muscle Culture

Susceptibility of human skeletal muscle culture to influenza virus infection

1978 ◽  
Vol 35 (1) ◽  
pp. 43-57 ◽  
Author(s):  
C.L. Armstrong ◽  
A.F. Miranda ◽  
K.C. Hsu ◽  
E.T. Gamboa
Keyword(s):  
Skeletal Muscle ◽  
Influenza Virus ◽  
Virus Infection ◽  
Human Skeletal Muscle ◽  
Influenza Virus Infection ◽  
Muscle Culture

Regulation of insulin action by an extract of Artemisia dracunculus L. in primary human skeletal muscle culture: A proteomics approach

2010 ◽  
Vol 24 (9) ◽  
pp. 1278-1284 ◽  
Author(s):  
Indu Kheterpal ◽  
Liana Coleman ◽  
Ginger Ku ◽  
Zhong Q. Wang ◽  
David Ribnicky ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Insulin Action ◽  
Human Skeletal Muscle ◽  
Artemisia Dracunculus ◽  
Muscle Culture ◽  
Proteomics Approach

scholarly journals Bioactives of Artemisia dracunculus L enhance cellular insulin signaling in primary human skeletal muscle culture

Metabolism ◽  
2008 ◽  
Vol 57 ◽  
pp. S58-S64 ◽  
Author(s):  
Zhong Q. Wang ◽  
David Ribnicky ◽  
Xian H. Zhang ◽  
Ilya Raskin ◽  
Yongmei Yu ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Insulin Signaling ◽  
Human Skeletal Muscle ◽  
Artemisia Dracunculus ◽  
Muscle Culture

LOCATION OF SUCCINIC DEHYDROGENASE IN SKELETAL MUSCLE CULTURE OF CHICK EMBRYOS

1966 ◽  
Vol 64 (1-3) ◽  
pp. 282-294 ◽  
Author(s):  
N.I. Germino ◽  
J.P. Wahrmann ◽  
H. D’albora
Keyword(s):  
Skeletal Muscle ◽  
Succinic Dehydrogenase ◽  
Chick Embryos ◽  
Muscle Culture

scholarly journals Artemisia dracunculusL. extract ameliorates insulin sensitivity by attenuating inflammatory signalling in human skeletal muscle culture

2014 ◽  
Vol 16 (8) ◽  
pp. 728-738 ◽  
Author(s):  
B. Vandanmagsar ◽  
K. R. Haynie ◽  
S. E. Wicks ◽  
E. M. Bermudez ◽  
T. M. Mendoza ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Insulin Sensitivity ◽  
Human Skeletal Muscle ◽  
Muscle Culture ◽  
Inflammatory Signalling

scholarly journals Adult fast myosin pattern and Ca2+-induced slow myosin pattern in primary skeletal muscle culture

1997 ◽  
Vol 94 (8) ◽  
pp. 4205-4210 ◽  
Author(s):  
H.-P. Kubis ◽  
E.-A. Haller ◽  
P. Wetzel ◽  
G. Gros
Keyword(s):  
Skeletal Muscle ◽  
Slow Myosin ◽  
Fast Myosin ◽  
Muscle Culture

scholarly journals PO-112 The exploration of constitutively expressed myokines utilizing tissue-engineered skeletal muscle

2018 ◽  
Vol 1 (4) ◽  
Author(s):  
Tomohiro Nakamura ◽  
Tatsuki Shibahara ◽  
Aya Takamori ◽  
Aki Nunomiya ◽  
Makoto Miyata ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Skeletal Muscle ◽  
Molecular Weight ◽  
Microarray Analysis ◽  
Culture Media ◽  
C2c12 Cells ◽  
Low Molecular Weight ◽  
Rt Pcr ◽  
Culture Cells ◽  
Muscle Culture

Objective Recent evidence has identified skeletal muscle as a secretory organ. Many myokines, which are bioactive substances secreted from skeletal muscle, have been identified in plane muscle culture cells. Compared to the plane muscle culture cells, the tissue-engineered muscle is an excellent model as culture system mimicked native skeletal muscle. However, constitutively expressed genes and secreted compounds from tissue-engineered muscle have not been analyzed sufficiently. The purposes of this study were 1) to clarify kinetics of constitutively secreted compounds, and 2) to explore constitutively expressed genes in the tissue-engineered muscle. Methods C2C12 cells embedded within collagen gel solution were placed between two tendons made up of elastase-treated acelluar porcine blood vessel. The constructs were cultured in growth media for 2 days and cultured in differentiation media for 6 days. To compare with plane culture cells, C2C12 cells were cultured in plane under the same condition as the construct. The culture media were obtained, and analyzed by MALDI-TOF Mass Spectrometry. Furthermore, constitutively up-regulated genes in tissue-engineered skeletal muscle were explored based on microarray analysis and confirmed by RT-PCR. Results MALDI-TOF Mass Spectrometry revealed that the number of detected peaks in tissue-engineered muscle was abundant compared to that of plane muscle culture cells, especially at range of low molecular weight. Furthermore, the detected peaks were substantially different among these culture media and specific peaks were identified in tissue-engineered muscle. Based on microarray analysis, the transcription of cholecystokinin identified, and confirmed the up-regulation in tissue-engineered skeletal muscle by RT-PCR. Conclusions These results suggested that the tissue-engineered muscle constitutively secreted many compounds compared to plane culture cells, especially at range of low molecular weight. Furthermore, the transcription of cholecystokinin was up-regulated in tissue-engineered skeletal muscle. Besides of the plane muscle culture cells, it is possible to expect to obtain novel myokines utilizing tissue-engineered muscle.

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