Real-Time PCR-Based Quantitation Method for the Genetically Modified Soybean Line GTS 40-3-2

2016 ◽  
pp. 249-257
Author(s):  
Kazumi Kitta ◽  
Reona Takabatake ◽  
Junichi Mano
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Genetically Modified ◽  
Soybean Line ◽  
Genetically Modified Soybean ◽  
Quantitation Method

scholarly journals Effect of Feeding Cows Genetically Modified Maize on the Bacterial Community in the Bovine Rumen

2007 ◽  
Vol 73 (24) ◽  
pp. 8012-8017 ◽  
Author(s):  
S. Wiedemann ◽  
P. Gürtler ◽  
C. Albrecht
Keyword(s):  
Bacterial Community ◽  
Real Time ◽  
Real Time Pcr ◽  
Genetically Modified ◽  
Genetically Modified Maize ◽  
Bacterial Strains ◽  
Maize Silage ◽  
Bovine Rumen ◽  
Gm Maize ◽  
Effect Of Feeding

ABSTRACT Rumen-cannulated cows (n = 4) were fed successively silage made from either conventional or genetically modified (GM) maize. Results revealed no effects of GM maize on the dynamics of six ruminal bacterial strains (investigated by real-time PCR) compared to the conventional maize silage.

scholarly journals Quantification of Genetically Modified Soybeans Using a Combination of a Capillary-Type Real-Time PCR System and a Plasmid Reference Standard

2006 ◽  
Vol 70 (4) ◽  
pp. 821-827 ◽  
Author(s):  
Akie TOYOTA ◽  
Hiroshi AKIYAMA ◽  
Mitsunori SUGIMURA ◽  
Takahiro WATANABE ◽  
Hiroyuki KIKUCHI ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Genetically Modified ◽  
Reference Standard ◽  
Capillary Type ◽  
Genetically Modified Soybeans

Detection by real-time PCR and pyrosequencing of the cry1Ab and cry1Ac genes introduced in genetically modified (GM) constructs

2017 ◽  
Vol 34 (8) ◽  
pp. 1398-1409 ◽  
Author(s):  
Frederic Debode ◽  
Eric Janssen ◽  
Claude Bragard ◽  
Gilbert Berben
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Genetically Modified

scholarly journals Tendency for Interlaboratory Precision in the GMO Analysis Method Based on Real-Time PCR

2010 ◽  
Vol 93 (2) ◽  
pp. 734-749 ◽  
Author(s):  
Takashi Kodama ◽  
Yasunori Kurosawa ◽  
Kazumi Kitta ◽  
Shigehiro Naito
Keyword(s):  
Standard Deviation ◽  
Real Time ◽  
Dna Extraction ◽  
Real Time Pcr ◽  
Quantitative Methods ◽  
Genetically Modified ◽  
Performance Criterion ◽  
Gm Crops ◽  
Evaluation Procedure ◽  
Method Performance

Abstract The Horwitz curve estimates interlaboratory precision as a function only of concentration, and is frequently used as a method performance criterion in food analysis with chemical methods. The quantitative biochemical methods based on real-time PCR require an analogous criterion to progressively promote method validation. We analyzed the tendency of precision using a simplex real-time PCR technique in 53 collaborative studies of seven genetically modified (GM) crops. Reproducibility standard deviation (SR) and repeatability standard deviation (Sr) of the genetically modified organism (GMO) amount () was more or less independent of GM crops (i.e., maize, soybean, cotton, oilseed rape, potato, sugar beet, and rice) and evaluation procedure steps. Some studies evaluated whole steps consisting of DNA extraction and PCR quantitation, whereas others focused only on the PCR quantitation step by using DNA extraction solutions. Therefore, SR and Sr for GMO amount () are functions only of concentration similar to the Horwitz curve. We proposed SR 0.1971C0.8685 and Sr 0.1478C0.8424, where C is the GMO amount (). We also proposed a method performance index in GMO quantitative methods that is analogous to the Horwitz Ratio.

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