Engineering Small-Scale and Scaffold-Based Bone Organs via Endochondral Ossification Using Adult Progenitor Cells

2016 ◽  
pp. 413-424 ◽  
Author(s):  
Celeste Scotti ◽  
Beatrice Tonnarelli ◽  
Adam Papadimitropoulos ◽  
Elia Piccinini ◽  
Atanas Todorov ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Endochondral Ossification ◽  
Small Scale

Loss of ephrinB1 in osteogenic progenitor cells impedes endochondral ossification and compromises bone strength integrity during skeletal development

Bone ◽  
2016 ◽  
Vol 93 ◽  
pp. 12-21 ◽  
Author(s):  
Thao M. Nguyen ◽  
Agnieszka Arthur ◽  
Sharon Paton ◽  
Sarah Hemming ◽  
Romana Panagopoulos ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Bone Strength ◽  
Endochondral Ossification ◽  
Skeletal Development

Quality evaluation of umbilical cord blood progenitor cells cryopreserved with a small-scale automated liquid nitrogen system

Cryobiology ◽  
2008 ◽  
Vol 57 (2) ◽  
pp. 178-181 ◽  
Author(s):  
Junko Miura ◽  
Masayoshi Minegishi ◽  
Tsuneo Itoh ◽  
Tamie Kitaura ◽  
Narumi Fukawa ◽  
...  
Keyword(s):  
Cord Blood ◽  
Progenitor Cells ◽  
Liquid Nitrogen ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Quality Evaluation ◽  
Small Scale

Small-Scale Mass Spectrometry-Based Phosphoproteomic Analysis of Primary Hematopoietic Stem and Progenitor Cells to Identify Critical Regulators of HSPC Mobilization and Leukemogenesis

Blood ◽  
2014 ◽  
Vol 124 (21) ◽  
pp. 4336-4336
Author(s):  
Leo D. Wang ◽  
Phi Nguyen ◽  
Scott B Ficarro ◽  
John Hutchinson ◽  
Oliver Hofmann ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Stem Cells ◽  
Progenitor Cells ◽  
Conflicts Of Interest ◽  
Small Scale ◽  
Cellular Functions ◽  
Hematopoietic Stem ◽  
Primary Mouse ◽  
Stem And Progenitor Cells ◽  
Blood Stem Cells

Abstract Cellular functions are largely effected by proteins, but protein-level analysis of hematopoietic stem and progenitor cell (HSPC) functions has historically been challenged by the difficulty of performing comprehensive and robust proteomic studies of rare cell populations. To confront this challenge, we developed a novel nanoscale multidimensional mass spectrometry-based phosphoproteomic platform that allows, for the first time, comprehensive and unbiased analysis of the activated protein circuits in blood stem cells, as assessed by protein phosphorylation status. We used this platform to interrogate the proteomic features responsible for the growth and maintenance of hematopoietic progenitors. Our analysis pipeline is capable of returning 12,000 unique phosphopeptide sequences (corresponding to several thousand proteins) from an input of 400,000 FACS-sorted primary mouse HSPCs. Among these phosphorylated proteins, the novel Rac-GAP Arhgap25 emerged as an important regulator of mobilization in HSPCs. Arhgap25 is phosphorylated upon treatment of HSPCs with a standard cyclophosphamide-GCSF mobilization protocol. Germline deletion of Arhgap25 in mice impairs HSPC egress from the bone marrow, both at rest and after mobilizing stimuli. These findings validate the use of this platform in the discovery of new therapeutic targets in hematopoiesis, and present a clear pathway for identifying novel targets in other rare subsets of human progenitor cells, including leukemia stem cells. Disclosures No relevant conflicts of interest to declare.

scholarly journals Periosteum-derived Osteocrin regulates bone growth through both endochondral ossification and intramembranous ossification

2019 ◽  
Author(s):  
Haruko Watanabe-Takano ◽  
Hiroki Ochi ◽  
Ayano Chiba ◽  
Ayaka Matsuo ◽  
Yugo Kanai ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Natriuretic Peptide ◽  
Endochondral Ossification ◽  
Bone Growth ◽  
Long Bone ◽  
Short Axis ◽  
Intramembranous Ossification ◽  
Peptide Receptor ◽  
Multipotent Progenitor Cells ◽  
Cortical Regions

ABSTRACTDuring development of long bones, two mechanistically distinct processes contribute to long- and short-axis growth. Endochondral ossification in the growth plate leads to the long-axis growth, while intramembranous ossification including apposition in the periosteum regulates the short axis growth. Here, we show that periosteal osteoblast-derived secretory peptide, Osteocrin (OSTN), promotes both types of long bone growth through potentiation of signaling by C-type natriuretic peptide (CNP), because OSTN inhibits the clearance of CNP by binding to natriuretic peptide receptor 3 (NPR3). The mice lacking OSTN showed less bone mass in trabecular and cortical regions than the control mice, suggesting the dual functions of OSTN in long bone growth. We found that OSTN regulated trabecular bone formation by inducing proliferation and maturation of chondrocytes possibly through enhancing CNP-dependent signaling. Besides the contribution of OSTN to long axis growth, we demonstrated that OSTN together with CNP induced osteoblast differentiation of periosteum-derived multipotent progenitor cells expressing NPR3. These data suggest that OSTN induces long bone growth through endochondral ossification and osteoblast specification of multipotent progenitor cells in the periosteum.

Reasons to strike first

2019 ◽  
Vol 42 ◽  
Author(s):  
William Buckner ◽  
Luke Glowacki
Keyword(s):  
Intergroup Conflict ◽  
Small Scale

Abstract De Dreu and Gross predict that attackers will have more difficulty winning conflicts than defenders. As their analysis is presumed to capture the dynamics of decentralized conflict, we consider how their framework compares with ethnographic evidence from small-scale societies, as well as chimpanzee patterns of intergroup conflict. In these contexts, attackers have significantly more success in conflict than predicted by De Dreu and Gross's model. We discuss the possible reasons for this disparity.

Sign in / Sign up

Export Citation Format

Share Document