Neutralization Assay for Chikungunya Virus Infection: Plaque Reduction Neutralization Test

2016 ◽  
pp. 273-282 ◽  
Author(s):  
Nor Azila Muhammad Azami ◽  
Meng Ling Moi ◽  
Tomohiko Takasaki
Keyword(s):  
Virus Infection ◽  
Chikungunya Virus ◽  
Neutralization Test ◽  
Neutralization Assay ◽  
Plaque Reduction Neutralization Test

scholarly journals Comparison of JEV neutralization assay using pseudotyped JEV with the conventional plaque-reduction neutralization test

2014 ◽  
Vol 52 (5) ◽  
pp. 435-440 ◽  
Author(s):  
Hee-Jung Lee ◽  
Kyung-Il Min ◽  
Ki Hoon Park ◽  
Hyo Jung Choi ◽  
Min-Kyoung Kim ◽  
...  
Keyword(s):  
Neutralization Test ◽  
Neutralization Assay ◽  
Plaque Reduction Neutralization Test

scholarly journals Serum Neutralization Assay Can Efficiently Replace Plaque Reduction Neutralization Test for Detection and Quantitation of West Nile Virus Antibodies in Human and Animal Serum Samples

2014 ◽  
Vol 21 (10) ◽  
pp. 1460-1462 ◽  
Author(s):  
Annapia Di Gennaro ◽  
Alessio Lorusso ◽  
Claudia Casaccia ◽  
Annamaria Conte ◽  
Federica Monaco ◽  
...  
Keyword(s):  
West Nile Virus ◽  
Neutralization Test ◽  
Neutralization Assay ◽  
Serum Samples ◽  
West Nile ◽  
Plaque Reduction Neutralization Test ◽  
Serum Neutralization ◽  
Human Sera

ABSTRACTA serum neutralization assay (SN) was compared with the official plaque reduction neutralization test for the quantitation of West Nile virus antibodies. A total of 1,348 samples from equid sera and 38 from human sera were tested by these two methods. Statistically significant differences were not observed, thus supporting the use of SN for routine purposes.

scholarly journals Development of a Pseudotyped-Lentiviral-Vector-Based Neutralization Assay for Chikungunya Virus Infection

2013 ◽  
Vol 51 (5) ◽  
pp. 1389-1395 ◽  
Author(s):  
N. Kishishita ◽  
N. Takeda ◽  
A. Anuegoonpipat ◽  
S. Anantapreecha
Keyword(s):  
Virus Infection ◽  
Chikungunya Virus ◽  
Lentiviral Vector ◽  
Neutralization Assay

scholarly journals Chikungunya Virus Infection in Traveler to Australia

2007 ◽  
Vol 13 (3) ◽  
pp. 509-510 ◽  
Author(s):  
Julian D. Druce ◽  
Douglas F. Johnson ◽  
Thomas Tran ◽  
Michael J. Richards ◽  
Christopher J. Birch
Keyword(s):  
Virus Infection ◽  
Chikungunya Virus

scholarly journals Temperature modulates innate immunity in Aedes aegypti during chikungunya virus infection

2020 ◽  
Vol 101 ◽  
pp. 489
Author(s):  
B.R. Wimalasiri-Yapa ◽  
F. Frentiu ◽  
L. Stassen ◽  
R. Gumiel
Keyword(s):  
Innate Immunity ◽  
Virus Infection ◽  
Aedes Aegypti ◽  
Chikungunya Virus

scholarly journals A trend of dropping anti‐SARS‐CoV‐2 plaque reduction neutralization test titers over time in Canadian convalescent plasma donors

Transfusion ◽  
2021 ◽  
Author(s):  
Steven J. Drews ◽  
Dana V. Devine ◽  
Janet McManus ◽  
Emelissa Mendoza ◽  
Kathy Manguiat ◽  
...  
Keyword(s):  
Neutralization Test ◽  
Plaque Reduction Neutralization Test ◽  
Over Time

scholarly journals Kinetic Analysis of Mouse Brain Proteome Alterations Following Chikungunya Virus Infection before and after Appearance of Clinical Symptoms

PLoS ONE ◽  
2014 ◽  
Vol 9 (3) ◽  
pp. e91397 ◽  
Author(s):  
Christophe Fraisier ◽  
Penelope Koraka ◽  
Maya Belghazi ◽  
Mahfoud Bakli ◽  
Samuel Granjeaud ◽  
...  
Keyword(s):  
Virus Infection ◽  
Kinetic Analysis ◽  
Mouse Brain ◽  
Chikungunya Virus ◽  
Clinical Symptoms ◽  
Before And After ◽  
Brain Proteome

scholarly journals Chikungunya Virus Infection: First Detection of Imported and Autochthonous Cases in Panama

2015 ◽  
Vol 92 (3) ◽  
pp. 482-485 ◽  
Author(s):  
Yamilka Díaz ◽  
Ana Belén Araúz ◽  
Ana Margarita Botello ◽  
Sandra López-Vergès ◽  
Lizbeth Cerezo ◽  
...  
Keyword(s):  
Virus Infection ◽  
Chikungunya Virus

scholarly journals Evaluation of six commercial SARS-CoV-2 serology assays detecting different antibodies for clinical testing and serosurveillance

2021 ◽  
Author(s):  
Suellen Nicholson ◽  
Theo Karapanagiotidis ◽  
Arseniy Khvorov ◽  
Celia Douros ◽  
Francesca Mordant ◽  
...  
Keyword(s):  
Cell Culture ◽  
Neutralization Test ◽  
Serological Test ◽  
Neutralizing Antibody ◽  
Neutralization Assay ◽  
Virus Neutralization Test ◽  
Virus Neutralization ◽  
Binding Assays ◽  
Humoral Immune ◽  
A Cell

Abstract Background Serological testing for SARS-CoV-2 complements nucleic acid tests for patient diagnosis and enables monitoring of population susceptibility to inform the COVID-19 pandemic response. It is important to understand the reliability of assays with different antigen or antibody targets to detect humoral immunity after SARS-CoV-2 infection and to understand how antibody (Ab) binding assays compare to those detecting neutralizing antibody (nAb), particularly as we move into the era of vaccines. Methods We evaluated the performance of six commercially available Enzyme-linked Immunosorbent Assays (ELISAs), including a surrogate virus neutralization test (sVNT), for detection of SARS-CoV-2 immunoglobulins (IgA, IgM, IgG), total or nAb. A result subset was compared to a cell culture-based microneutralisation (MN) assay. We tested sera from patients with prior RT-PCR confirmed SARS-CoV-2 infection, pre-pandemic sera and potential cross-reactive sera from patients with other non-COVID-19 acute infections. Results For sera collected > 14 days post-symptom onset, the assay achieving the highest sensitivity was the Wantai total Ab at 100% (95% confidence interval: 94.6-100) followed by 93.1% for Euroimmun NCP-IgG, 93.1% for GenScript sVNT, 90.3% for Euroimmun S1-IgG, 88.9% for Euroimmun S1-IgA and 83.3% for Wantai IgM. Specificity for the best performing assay was 99.5% for the Wantai total Ab and for the lowest performing assay was 97.1% for sVNT (as per IFU). The Wantai Total Ab had the best agreement with MN at 98% followed by Euroimmun S1-IgA, Euro NCP-IgG and sVNT (as per IFU) with (97%, 97% and 95% respectively) and Wantai IgM having the poorest agreement at 93%. Conclusion Performance characteristics of the SARS-CoV-2 serology assays detecting different antibody types are consistent with those found in previously published reports. Evaluation of the surrogate virus neutralization test in comparison to the Ab binding assays and a cell culture-based neutralization assay showed good result correlation between all assays. However correlation between the cell-based neutralization test and some assays detecting Ab’s not specifically involved in neutralization was higher than with the sVNT. This study demonstrates the reliability of different assays to detect the humoral immune response following SARS-CoV-2 infection, which can be used to optimise serological test algorithms for assessing antibody responses post SARS-CoV-2 infection or vaccination.

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