scholarly journals Serum Neutralization Assay Can Efficiently Replace Plaque Reduction Neutralization Test for Detection and Quantitation of West Nile Virus Antibodies in Human and Animal Serum Samples

2014 ◽  
Vol 21 (10) ◽  
pp. 1460-1462 ◽  
Author(s):  
Annapia Di Gennaro ◽  
Alessio Lorusso ◽  
Claudia Casaccia ◽  
Annamaria Conte ◽  
Federica Monaco ◽  
...  
Keyword(s):  
West Nile Virus ◽  
Neutralization Test ◽  
Neutralization Assay ◽  
Serum Samples ◽  
West Nile ◽  
Plaque Reduction Neutralization Test ◽  
Serum Neutralization ◽  
Human Sera

ABSTRACTA serum neutralization assay (SN) was compared with the official plaque reduction neutralization test for the quantitation of West Nile virus antibodies. A total of 1,348 samples from equid sera and 38 from human sera were tested by these two methods. Statistically significant differences were not observed, thus supporting the use of SN for routine purposes.

scholarly journals Persistence of Antibodies to West Nile Virus in Naturally Infected Rock Pigeons (Columba livia)

2005 ◽  
Vol 12 (5) ◽  
pp. 665-667 ◽  
Author(s):  
Samantha E. J. Gibbs ◽  
Douglas M. Hoffman ◽  
Lillian M. Stark ◽  
Nicole L. Marlenee ◽  
Bradley J. Blitvich ◽  
...  
Keyword(s):  
West Nile Virus ◽  
Immunosorbent Assay ◽  
Neutralization Test ◽  
Columba Livia ◽  
Maternal Antibodies ◽  
Enzyme Linked Immunosorbent Assay ◽  
West Nile ◽  
Entire Study ◽  
Plaque Reduction Neutralization Test ◽  
Antibody Persistence

ABSTRACT Wild caught rock pigeons (Columba livia) with antibodies to West Nile virus were monitored for 15 months to determine antibody persistence and compare results of three serologic techniques. Antibodies persisted for the entire study as detected by epitope-blocking enzyme-linked immunosorbent assay and plaque reduction neutralization test. Maternal antibodies in squabs derived from seropositive birds persisted for an average of 27 days.

scholarly journals First serological evidence of West Nile virus among equines and birds in Kosovo, 2018-2019

2020 ◽  
Author(s):  
Agim Rexhepi ◽  
Kurtesh Sherifi ◽  
Kristaq Berxholi ◽  
Betim Xhekaj ◽  
Nesade Muja-Bajraktari ◽  
...  
Keyword(s):  
West Nile Virus ◽  
Neutralization Test ◽  
Virus Detection ◽  
Control Program ◽  
Serum Samples ◽  
West Nile ◽  
Health Authorities ◽  
Domestic Bird ◽  
Domestic Birds ◽  
Public Health Authorities

Abstract Background: This study was conducted to survey the presence of the West Nile virus (WNV) in Kosovo by serological testing of the healthy autochthonous equine population and virus detection in birds and mosquitoes. Between January 2018 and June 2019, 260 equine serum samples, 626 mosquitoes (60 pools), 50 domestic birds and 51 wild birds were collected from different regions of Kosovo. Equine and domestic bird serum samples were tested by IgG ELISA while mosquitoes and bird viscera were tested for WNV RNA by RT-PCR. Positive ELISA samples were confirmed by Plaque Reduction Neutralization Test (PRNT) and eight by Virus Neutralization Test (VNT). Results: This is the first report providing evidence of WNV antibodies among animals in Kosovo. WNV antibodies were present in 27 out of 260 equine sera (10.38%) and one out of 50 samples in domestic birds by ELISA and PRNT. Eight of 27 positive equine serum samples were confirmed by VNT. No WNV RNA was detected in birds or mosquitoes.Conclusions: The occurrence of WNV antibodies in autochthonous equines from all regions of Kosovo indicates that the virus is circulating within the country. Public health authorities should therefore plan a risk assessment and disease control program.

Serological investigations on West Nile virus in birds and horses in Shanghai, China

2012 ◽  
Vol 141 (3) ◽  
pp. 596-600 ◽  
Author(s):  
D. L. LAN ◽  
C. S. WANG ◽  
B. DENG ◽  
J. P. ZHOU ◽  
L. CUI ◽  
...  
Keyword(s):  
Public Health ◽  
West Nile Virus ◽  
Neutralization Test ◽  
Horse Serum ◽  
Cross Reactivity ◽  
Competitive Elisa ◽  
False Positives ◽  
Global Public Health ◽  
Serum Samples ◽  
West Nile

SUMMARYWest Nile virus (WNV) infection is an emerging zoonosis that threatens global public health. In this study, a total of 95 bird serum samples from 14 species and 341 horse serum samples were collected from 2008 to 2010 in Shanghai, China. All serum samples were screened initially for WNV-reactive antibodies using a competitive ELISA. The positive samples detected by ELISA were further confirmed using a plaque-reduction neutralization test (PRNT) for WNV and its most closely related flaviviruses in the area to avoid false positives due to cross-reactivity. Five (5·3%) of the bird serum samples and none (0·0%) of the horse serum samples tested positive for WNV antibodies. The findings strongly suggest that some of the birds, specifically the resident birds in China, had been exposed to WNV.

The continuous spread of West Nile virus (WNV): seroprevalence in asymptomatic horses

2009 ◽  
Vol 137 (8) ◽  
pp. 1163-1168 ◽  
Author(s):  
J. ALONSO-PADILLA ◽  
E. LOZA-RUBIO ◽  
E. ESCRIBANO-ROMERO ◽  
L. CÓRDOBA ◽  
S. CUEVAS ◽  
...  
Keyword(s):  
West Nile Virus ◽  
Gulf Of Mexico ◽  
Neutralization Test ◽  
Haemagglutination Inhibition ◽  
Igg Antibodies ◽  
West Nile ◽  
Plaque Reduction Neutralization Test ◽  
Northern Mexico ◽  
Independent Events ◽  
The Usa

SUMMARYWest Nile virus (WNV) was probably introduced in southern and northern Mexico from the USA in two independent events. Since then, WNV activity has been reported in several Mexican states bordering the USA and the Gulf of Mexico, but disease manifestations seen there in humans and equids are quite different to those observed in the USA. We have analysed WNV seroprevalence in asymptomatic, unvaccinated equids from two Mexican states where no data had been previously recorded. WNV IgG antibodies were detected in 31·6% (91/288) of equine sera from Chiapas and Puebla states (53·3% and 8·0%, respectively). Analysis by plaque reduction neutralization test (PRNT) showed good specificity (99·4%) and sensitivity (84·9%) with the ELISA results. Further analyses to detect antibodies against three different flaviviruses (WNV, St Louis encephalitis virus, Ilheus virus) by haemagglutination inhibition (HI) tests on a subset of 138 samples showed that 53% of the 83 HI-positive samples showed specific reaction to WNV. These data suggest continuous expansion of WNV through Mexico.

scholarly journals First serological evidence of West Nile virus among equines and birds in Kosovo, 2018-2019

2020 ◽  
Author(s):  
Agim Rexhepi ◽  
Kurtesh Sherifi ◽  
Kristaq Berxholi ◽  
Betim Xhekaj ◽  
Nesade Muja-Bajraktari ◽  
...  
Keyword(s):  
West Nile Virus ◽  
Neutralization Test ◽  
High Titer ◽  
Control Program ◽  
Serum Samples ◽  
West Nile ◽  
Health Authorities ◽  
Domestic Bird ◽  
Domestic Birds ◽  
Public Health Authorities

Abstract Background: This study was conducted to survey the presence of West Nile virus (WNV) in Kosovo by serological testing of apparently healthy local horses and attempts to detect viral nucleic acid in birds and mosquitoes. Methods: Between January 2018 and June 2019, 260 equine serum samples were collected, additionally 580 adult mosquitoes (53 pools) grouped in for genera, including Culex spp. (226 individuals; 26 pools), Aedes spp. (136 individuals; 16 pools), Anopheles spp . (184 individuals; 7 pools), Culiseta spp . (34 individuals; 4 pools). 50 domestic birds and 51 wild birds were collected from different regions of Kosovo. Equine and domestic bird serum samples were tested by flavivirus IgG ELISA while mosquitoes and bird viscera were tested for WNV RNA by RT-qPCR. All ELISA-positive results were confirmed by plaque reduction neutralization test (PRNT) and by virus neutralization test (VNT). Results: This is the first report providing evidence of WNV antibodies among animals in Kosovo. WNV antibodies were present in 27 out of 260 equine sera (10.38%) and one out of 50 samples in domestic birds by ELISA and PRNT. Eight of 27 positive equine serum samples with high titer neutralized WNV, but not USUV. No WNV RNA was detected in birds or mosquitoes. Conclusions: The occurrence of WNV antibodies in local equines from all regions of Kosovo indicates that the virus is circulating within the country. Public health authorities should therefore plan a risk assessment and disease control program. Keywords: West Nile virus, Kosovo, Equines, Birds, Mosquitoes.

scholarly journals Comparison of JEV neutralization assay using pseudotyped JEV with the conventional plaque-reduction neutralization test

2014 ◽  
Vol 52 (5) ◽  
pp. 435-440 ◽  
Author(s):  
Hee-Jung Lee ◽  
Kyung-Il Min ◽  
Ki Hoon Park ◽  
Hyo Jung Choi ◽  
Min-Kyoung Kim ◽  
...  
Keyword(s):  
Neutralization Test ◽  
Neutralization Assay ◽  
Plaque Reduction Neutralization Test

scholarly journals Is West Nile virus a potential cause of central nervous system infection in Brazil?

2010 ◽  
Vol 68 (5) ◽  
pp. 761-763 ◽  
Author(s):  
Cristiane N Soares ◽  
Mauro J. Cabral Castro ◽  
José M Peralta ◽  
Marcos R.G. de Freitas ◽  
Marzia Puccioni-Sohler
Keyword(s):  
West Nile Virus ◽  
Rio De Janeiro ◽  
Viral Encephalitis ◽  
Neutralization Test ◽  
Dengue Infection ◽  
Central Nervous System Infection ◽  
Viral Meningitis ◽  
Unknown Etiology ◽  
West Nile ◽  
The City

Meningitis and encephalitis are complications of West Nile virus (WNV) infection. Although WNV is endemic in North America, the virus has recently been reported in Colombia and Argentina. Investigation of WNV in Brazil is important since this virus has never been studied previously in this country. OBJECTIVE: To investigate the presence of WNV infection in viral encephalitis/meningitis cases of unknown etiology in the city of Rio de Janeiro, Brazil. METHOD: Thirty-seven adults with viral meningitis/encephalitis had their serum and CSF tested for WNV antibodies using the ELISA method. RESULTS: Only one case was WNV-positive, but this case was also positive for dengue. The plaque reduction neutralization test distinguished infections, and was negative for WNV. CONCLUSION: WNV can be confused with dengue infection. Their symptoms and neurological picture are similar. We did not find WNV in any patients with encephalitis and meningitis in the city of Rio de Janeiro. Up to now, it has not been detected in Brazil

Isolation from Human Sera in Egypt of a Virus Apparently Identical to West Nile Virus.

1951 ◽  
Vol 77 (4) ◽  
pp. 661-665 ◽  
Author(s):  
J. L. Melnick ◽  
J. R. Paul ◽  
J. T. Riordan ◽  
V. H. Barnett ◽  
N. Goldblum ◽  
...  
Keyword(s):  
West Nile Virus ◽  
West Nile ◽  
Human Sera

scholarly journals Varicella-zoster plaque assay and plaque reduction neutralization test by the immunoperoxidase technique

1976 ◽  
Vol 4 (5) ◽  
pp. 437-442
Author(s):  
G Gerna ◽  
R W Chambers
Keyword(s):  
Neutralization Test ◽  
Plaque Assay ◽  
Neutralizing Antibody ◽  
Immunoperoxidase Technique ◽  
Serum Samples ◽  
Plaque Reduction Neutralization Test ◽  
Varicella Zoster ◽  
Convalescent Phase ◽  
Antibody Titers ◽  
Indirect Immunoperoxidase

A new plaque assay for the quantitation of varicella-zoster virus and a plaque reduction neutralization test for the determination of neutralizing antibody titer have been developed using the indirect immunoperoxidase technique. As compared with the classical plaque assay using a solid overlay, the test gives earlier results since plaque counting can be performed on day 3 after the inoculation of cell cultures. In six patients with zoster infection, neutralizing antibody titers ranged from 1:20 to 1:40 before the onset of infection and reached high levels (1:320 to 1:5,120) during the convalescent phase of the disease. Complement-fixing (CF) titers were all negative (less than 1:8) in prezoster serum samples from the same patients and ranged from 1:128 to 1:2,048 in the convalescent-phase sera. In the two cases in which late serum samples were available, neutralizing antibody titers matched the preillness levels, whereas CF titers dropped to undetectable levels. Neither neutralizing nor CF antibody was detected in two sera from individuals with no history of varicella-zoster infection. No differences in virus titers or neutralizing antibody titers were observed between the immunoperoxidase and the classical plaque assays. The appropriate characterization of reagent specificity is required before routine application of the test.

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